PMID- 32250835
OWN - NLM
STAT- MEDLINE
DCOM- 20200408
LR  - 20200408
IS  - 1095-8630 (Electronic)
IS  - 0301-4797 (Linking)
VI  - 262
DP  - 2020 May 15
TI  - Elucidating sludge characteristic, substrate transformation and microbial 
      evolution in a two-sludge denitrifying phosphorus removal system under the impact 
      of HRT.
PG  - 110391
LID - S0301-4797(20)30326-1 [pii]
LID - 10.1016/j.jenvman.2020.110391 [doi]
AB  - Granule formation has been recognized as a promising biotechnology in 
      denitrifying phosphorus removal (DPR) systems by facilitating phosphorus 
      accumulation organisms (PAOs) especially denitrifying PAOs (DPAOs), and hydraulic 
      selection made this a more difficult task in continuous operation. This study 
      aimed at exploring the microscopic mechanism and putting forward an effective 
      strategy for DPR granulation under the impact of hydraulic retention time (HRT) 
      (12 h, 10 h, 8 h) in a novel Anaerobic Anoxic Oxic - Moving Bed Biofilm Reactor 
      (A(2)/O - MBBR) system. With the reduction of intracellular carbon storage 
      (COD(intra)) efficiency (88.58%-78.53%), nitrogen (N) (85.45%-79.11%) and 
      phosphorus (P) (96.55%-92.47%) removals both dropped, but it exhibited a growth 
      of anoxic phosphorus uptake rate (PUR(A)) (3.79-5.68 mg P/(gMLVSS.h)). The batch 
      tests associating with substrate transformation of poly-beta-hydroxyalkanoates 
      (PHA), glycogen (Gly) agreed well with the corresponding stoichiometry of 
      phosphorus release rate (PRR) (4.83-7.53 mg P/(gMLVSS.h)), PUR(A) 
      (3.55-5.43 mg P/(gMLVSS.h)), oxic phosphorus uptake rate (PUR(O)) 
      (6.08-6.21 mg P/(gMLVSS.h)), and DPAOs/PAOs ratios (57.17%-89.31%), indicating a 
      shift of microbial community. DPR granules gradually stabilized with low sludge 
      volume index (SVI(5)/SVI(30) ratio = 1.1-1.2), dense and compact structure, 
      higher P content (11.63%), more extracted extracellular polymeric substances 
      (EPS) (111.40-160.31 mg/gMLVSS) as proteins/polysaccharides (PN/PS) ratios 
      (1.70-3.47) increased, leading to better sludge settleability and cell 
      hydrophobicity. Fluorescence in situ hybridization (FISH) results showed that 
      PAOs (mainly Cluster I: 20.20%) were the dominant bacteria in the A(2)/O reactor 
      although a small amount of Defluviicoccus (3.18-3.48%) was responsible for 
      nitrite accumulation, while ammonium-oxidizing bacteria (AOB) (mainly 
      Nitrosomonas: 10.75%) and nitrite-oxidizing bacteria (NOB) (mainly Nitrospira: 
      15.06%) were enriched in the MBBR.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhang, Miao
AU  - Zhang M
AD  - College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 
      225127, PR China.
FAU - Zhu, Chenjie
AU  - Zhu C
AD  - College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 
      225127, PR China.
FAU - Pan, Ting
AU  - Pan T
AD  - College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 
      225127, PR China.
FAU - Fan, Yajun
AU  - Fan Y
AD  - Yangzhou Polytechnic Institute, Yangzhou, 225127, PR China.
FAU - Liu, Yizhong
AU  - Liu Y
AD  - Yangzhou Jieyuan Drainage Company Limited, Yangzhou, 225002, PR China.
FAU - He, Chengda
AU  - He C
AD  - College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 
      225127, PR China.
FAU - Gu, Xiaodan
AU  - Gu X
AD  - School of Environmental Science and Engineering, Suzhou University of Science and 
      Technology, Suzhou, 215009, PR China.
FAU - Wu, Jun
AU  - Wu J
AD  - College of Environmental Science and Engineering, Yangzhou University, Yangzhou, 
      225127, PR China. Electronic address: j.wu@yzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200306
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Biofilms
MH  - Bioreactors
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrogen
MH  - *Phosphorus
MH  - *Sewage
MH  - Waste Disposal, Fluid
OTO - NOTNLM
OT  - A(2)/O
OT  - Denitrifying phosphorus removal
OT  - Extracellular polymeric substance
OT  - FISH
OT  - Granular sludge
OT  - HRT
OT  - MBBR
COIS- Declaration of competing interests The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/04/07 06:00
MHDA- 2020/04/09 06:00
CRDT- 2020/04/07 06:00
PHST- 2019/12/18 00:00 [received]
PHST- 2020/02/05 00:00 [revised]
PHST- 2020/03/02 00:00 [accepted]
PHST- 2020/04/07 06:00 [entrez]
PHST- 2020/04/07 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
AID - S0301-4797(20)30326-1 [pii]
AID - 10.1016/j.jenvman.2020.110391 [doi]
PST - ppublish
SO  - J Environ Manage. 2020 May 15;262:110391. doi: 10.1016/j.jenvman.2020.110391. 
      Epub 2020 Mar 6.