PMID- 32250884
OWN - NLM
STAT- MEDLINE
DCOM- 20200506
LR  - 20200506
IS  - 1095-8630 (Electronic)
IS  - 0301-4797 (Linking)
VI  - 264
DP  - 2020 Jun 15
TI  - Effect of simulated acid rain on the stability of calcium carbonate immobilized 
      by microbial carbonate precipitation.
PG  - 110419
LID - S0301-4797(20)30353-4 [pii]
LID - 10.1016/j.jenvman.2020.110419 [doi]
AB  - The stability of carbonate products resulting from microbially induced carbonate 
      precipitation (MICP) under acid rain is under question. The present study 
      investigated the stability of CaCO(3) precipitated by MICP in soil under 
      simulated acid rain (SAR). Soils were treated continuously for two months with 
      four SAR pH levels: 3.5, 4.5, 5.5, and 7.0. During SAR, biostimulation using 
      nutrient broth containing urea and calcium chloride was adopted to ensure CaCO(3) 
      precipitation. At the end of treatments, soil samples from top and bottom layers 
      were analyzed for bacterial diversity by Illumina MiSeq sequencing, Fourier 
      transform infrared (FTIR) spectroscopy for identification of chemical functional 
      groups related to calcite precipitation, and X-ray diffraction (XRD) for 
      identification of the main crystalline phases. The analysis identified several 
      ureolytic bacteria mainly from Arthrobacter and Sporosarcina genera in 
      SAR-treated soils accelerated with biostimulation, and urease quantities of 
      greater than 300 mg NH(4)(+) per kg soil at all pH levels. The precipitation of 
      CaCO(3) was pronounced and its stability was maintained even when the pH was as 
      low as 3.5. The results obtained in this study are helpful to the scientific 
      community to ensure the immobilization of heavy metals with microbial carbonate 
      precipitation in soil under acid rain.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Chen, X
AU  - Chen X
AD  - Environmental Engineering Program, Guangdong Technion - Israel Institute of 
      Technology, Shantou, 515063, China.
FAU - Achal, V
AU  - Achal V
AD  - Environmental Engineering Program, Guangdong Technion - Israel Institute of 
      Technology, Shantou, 515063, China; Technion - Israel Institute of Technology, 
      Haifa, 32000, Israel. Electronic address: varenyam.achal@gtiit.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200330
PL  - England
TA  - J Environ Manage
JT  - Journal of environmental management
JID - 0401664
RN  - 0 (Acid Rain)
RN  - 0 (Carbonates)
RN  - 0 (Soil)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - *Acid Rain
MH  - Calcium Carbonate
MH  - Carbonates
MH  - Chemical Precipitation
MH  - Soil
MH  - *Sporosarcina
OTO - NOTNLM
OT  - Biostimulation
OT  - Calcite stability
OT  - Heavy metals
OT  - Simulated acid rain
OT  - Urease
COIS- Declaration of competing interests The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/04/07 06:00
MHDA- 2020/05/07 06:00
CRDT- 2020/04/07 06:00
PHST- 2019/10/21 00:00 [received]
PHST- 2020/02/24 00:00 [revised]
PHST- 2020/03/07 00:00 [accepted]
PHST- 2020/04/07 06:00 [pubmed]
PHST- 2020/05/07 06:00 [medline]
PHST- 2020/04/07 06:00 [entrez]
AID - S0301-4797(20)30353-4 [pii]
AID - 10.1016/j.jenvman.2020.110419 [doi]
PST - ppublish
SO  - J Environ Manage. 2020 Jun 15;264:110419. doi: 10.1016/j.jenvman.2020.110419. 
      Epub 2020 Mar 30.