PMID- 32251565
OWN - NLM
STAT- MEDLINE
DCOM- 20200518
LR  - 20220413
IS  - 1009-3591 (Print)
IS  - 1009-3591 (Linking)
VI  - 25
IP  - 12
DP  - 2019 Dec
TI  - [Shenjing Guben Pills for repairing testicular spermatogenic injury induced by 
      oxidative stress in rats].
PG  - 1118-1125
AB  - OBJECTIVE: To investigate the antioxidative and spermatogenesis-repairing effects 
      of Shenjing Guben Pills (SGP), a Chinese medicine for invigorating the kidney and 
      blood circulation, on the testis, epididymis and sperm in rats with oxidative 
      stress injury (OSI) induced by cadmium chloride. METHODS: Seventy-two male Wistar 
      rats were equally randomized into six groups: normal control, OSI model control, 
      Wuzi Yanzong Pills (WYP) and low-, medium- and high-dose SGP. The OSI model was 
      made in the latter five groups by intraperitoneal injection of cadmium chloride 
      at 1 mg/kg, and 24 hours later, the rats of the normal and model control groups 
      treated intragastrically with 0.9% normal saline, those of the WYP group with WYP 
      at 4.5 g/kg/d, and those of the low-, medium- and high-dose SGP groups with SGP 
      at 2.8, 5.6 and 11.2 g/kg/d, respectively, all for 56 days. Then, all the animals 
      were sacrificed for obtainment of the visceral indexes and histopathological 
      changes of the testis, epididymis and seminal vesicle, measurement of sperm 
      concentration and motility and the percentage of morphologically normal sperm 
      (MNS) in the epididymis, and determination of the levels of glutathione 
      perox-idase (GSH-PX), superoxide dismutase (SOD), malondial-dehyde aldehyde (MDA) 
      and serum testosterone (T). RESULTS: Compared with the OSI model controls, the 
      rats in the high-, medium- and low-dose SGP groups showed significantly higher 
      visceral indexes of the testis (［0.237 +/- 0.098］ vs ［0.403 +/- 0.090］, ［0.357 +/- 
      0.150］ and ［0.348 +/- 0.140］ g/100 g, P < 0.05) and seminal vesicle (［0.241 +/- 
      0.118］ vs ［0.347 +/- 0.115］, ［0.336 +/- 0.090］ and ［0.320 +/- 0.065］ g/100 g, P < 0.05) 
      and those of the high-dose SGP group in the epididymal index (［0.099 +/- 0.088］ vs 
      ［0.156 +/- 0.030］ g/100 g, P < 0.05). In comparison with the OSI model controls, 
      the animals of the high-, medium- and low-dose SGP groups exhibited significant 
      increases in sperm concentration (［10.5 +/- 17.7］ vs ［58.1 +/- 32.2］, ［36.0 +/- 36.2］ 
      and ［31.9 +/- 32.7］ x10(6)/ml, P < 0.05) and serum T (［2.56 +/- 0.75］ vs ［3.62 +/- 0.96］, 
      ［3.48 +/- 1.33］ and ［3.24 +/- 0.83］ nmol/L, P < 0.05 or P < 0.01), and those of the 
      high- and medium-dose SCG groups in total sperm motility (［9.5 +/- 13.0］% vs ［26.5 
      +/- 15.5］% and ［18.9 +/- 8.2］%, P < 0.05) and MNS (［36.2 +/- 40.2］% vs ［85.3 +/- 23.3］% 
      and ［65.8 +/- 28.1］%, P < 0.05) and the levels GSH-PX (［3.62 +/- 2.22］ vs ［5.70 +/- 
      1.73］ and ［5.42 +/- 2.35］ U/mg prot, P < 0.05 ) and SOD (［41.3 +/- 8.8］ vs ［52.7 +/- 
      14.6］ and ［51.3 +/- 14.7］ U/mg prot, P < 0.05). The MDA level, however, was 
      markedly decreased in the high-, medium- and low-dose SGP groups (［0.41 +/- 0.29］, 
      ［0.44 +/- 0.19］ and ［0.47 +/- 0.20］ nmol/mg prot) as compared with that in the OSI 
      model controls (［0.69 +/- 0.28］ nmol/mg prot) (P < 0.05). Histopathological 
      examinations manifested coagulative necrosis, calcification and disappearance of 
      spermatogenic and Sertoli cells in the seminiferous tubules of the OSI model 
      controls, with decreased intraluminal secretions and atrophic epithelial papillae 
      in the seminal vesicles and non-sperm cells in the narrowed lumens of the 
      atrophic epididymis. With the increased dose of SGP, the proportion of normal 
      seminiferous tubules was enlarged, the epithelia of the seminal vesicle became 
      column-shaped again, and the epididymal lumens grew lager with more sperm cells, 
      which indicated a dose-dependent therapeutic efficacy. Medium- and high-dose SGP 
      achieved a significantly better effect than WYP. CONCLUSIONS: Shenjing Guben 
      Pills can antagonize oxidative stress, elevate the levels of testicular 
      antioxidant enzymes and serum T, repair pathological injury of the testis, 
      epididymis and seminal vesicle, and improve semen quality and spermatogenic 
      function.
FAU - Deng, Yu-Fei
AU  - Deng YF
AD  - The First School of Clinical Medicine, Guangzhou University of Chinese Medicine 
      The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510405, China.
FAU - Weng, Zhi-Wei
AU  - Weng ZW
AD  - Department of Reproductive Medicine, The First Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong 510405, China.
FAU - Liang, Ai-Jun
AU  - Liang AJ
AD  - Department of Reproductive Medicine, The First Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong 510405, China.
FAU - Li, Ling
AU  - Li L
AD  - Laboratory Center, The First Affiliated Hospital of Guangzhou University of 
      Chinese Medicine, Guangzhou, Guangdong 510405, China.
FAU - Weng, Hao-Wei
AU  - Weng HW
AD  - The First School of Clinical Medicine, Guangzhou University of Chinese Medicine 
      The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510405, China.
FAU - Liu, Zhi-Dan
AU  - Liu ZD
AD  - The First School of Clinical Medicine, Guangzhou University of Chinese Medicine 
      The First Affiliated Hospital of Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510405, China.
FAU - Zhou, Shao-Hu
AU  - Zhou SH
AD  - Department of Reproductive Medicine, The First Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou, Guangdong 510405, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Nan Ke Xue
JT  - Zhonghua nan ke xue = National journal of andrology
JID - 101093592
RN  - 0 (Drugs, Chinese Herbal)
RN  - J6K4F9V3BA (Cadmium Chloride)
SB  - IM
MH  - Animals
MH  - Cadmium Chloride
MH  - Drugs, Chinese Herbal/*therapeutic use
MH  - Epididymis/*drug effects
MH  - Humans
MH  - Male
MH  - Oxidative Stress/*drug effects
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Semen Analysis
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatogenesis/*drug effects
MH  - Spermatozoa
MH  - Testis/*drug effects
OTO - NOTNLM
OT  - invigorating kidney and blood circulation
OT  - oxidative stress
OT  - rat
OT  - spermatogenic injury
OT  - Shenjing Guben Pills
EDAT- 2020/04/07 06:00
MHDA- 2020/05/19 06:00
CRDT- 2020/04/07 06:00
PHST- 2020/04/07 06:00 [entrez]
PHST- 2020/04/07 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nan Ke Xue. 2019 Dec;25(12):1118-1125.