PMID- 32252443
OWN - NLM
STAT- MEDLINE
DCOM- 20210219
LR  - 20210219
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 12
IP  - 4
DP  - 2020 Apr 2
TI  - Differences in Tissue and Species Tropism of Reptarenavirus Species Studied by 
      Vesicular Stomatitis Virus Pseudotypes.
LID - 10.3390/v12040395 [doi]
LID - 395
AB  - Reptarenaviruses cause Boid Inclusion Body Disease (BIBD), and co-infections by 
      several reptarenaviruses are common in affected snakes. Reptarenaviruses have 
      only been found in captive snakes, and their reservoir hosts remain unknown. In 
      affected animals, reptarenaviruses appear to replicate in most cell types, but 
      their complete host range, as well as tissue and cell tropism are unknown. As 
      with other enveloped viruses, the glycoproteins (GPs) present on the virion's 
      surface mediate reptarenavirus cell entry, and therefore, the GPs play a critical 
      role in the virus cell and tissue tropism. Herein, we employed single cycle 
      replication, GP deficient, recombinant vesicular stomatitis virus (VSV) 
      expressing the enhanced green fluorescent protein (scrVSV∆G-eGFP) pseudotyped 
      with different reptarenavirus GPs to study the virus cell tropism. We found that 
      scrVSV∆G-eGFPs pseudotyped with reptarenavirus GPs readily entered mammalian cell 
      lines, and some mammalian cell lines exhibited higher, compared to snake cell 
      lines, susceptibility to reptarenavirus GP-mediated infection. Mammarenavirus GPs 
      used as controls also mediated efficient entry into several snake cell lines. Our 
      results confirm an important role of the virus surface GP in reptarenavirus cell 
      tropism and that mamma-and reptarenaviruses exhibit high cross-species 
      transmission potential.
FAU - Korzyukov, Yegor
AU  - Korzyukov Y
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
FAU - Iheozor-Ejiofor, Rommel
AU  - Iheozor-Ejiofor R
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
FAU - Levanov, Lev
AU  - Levanov L
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
FAU - Smura, Teemu
AU  - Smura T
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
FAU - Hetzel, Udo
AU  - Hetzel U
AD  - Vetsuisse Faculty, Institute of Veterinary Pathology, University of Zurich, 8006 
      Zurich, Switzerland.
AD  - Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University 
      of Helsinki, 00100 Helsinki, Finland.
FAU - Szirovicza, Leonora
AU  - Szirovicza L
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
FAU - de la Torre, Juan Carlos
AU  - de la Torre JC
AD  - Department of Immunology and Microbial Science IMM-6, The Scripps Research 
      Institute, La Jolla, CA 92037, USA.
FAU - Martinez-Sobrido, Luis
AU  - Martinez-Sobrido L
AD  - Texas Biomedical Research Institute, San Antonio, TX 78227, USA.
FAU - Kipar, Anja
AU  - Kipar A
AD  - Vetsuisse Faculty, Institute of Veterinary Pathology, University of Zurich, 8006 
      Zurich, Switzerland.
AD  - Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University 
      of Helsinki, 00100 Helsinki, Finland.
FAU - Vapalahti, Olli
AU  - Vapalahti O
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
AD  - Department of Veterinary Biosciences, Faculty of Veterinary Medicine, University 
      of Helsinki, 00100 Helsinki, Finland.
AD  - Department of Virology, Helsinki University Hospital, University of Helsinki, 
      00100 Helsinki, Finland.
FAU - Hepojoki, Jussi
AU  - Hepojoki J
AD  - Medicum, Department of Virology, University of Helsinki, 00100 Helsinki, Finland.
AD  - Vetsuisse Faculty, Institute of Veterinary Pathology, University of Zurich, 8006 
      Zurich, Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200402
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - A549 Cells
MH  - Animals
MH  - Arenaviridae/genetics/*physiology
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Green Fluorescent Proteins/genetics
MH  - HEK293 Cells
MH  - Humans
MH  - Snakes
MH  - Vero Cells
MH  - Vesiculovirus/genetics/*physiology
MH  - *Viral Envelope Proteins/genetics/metabolism
MH  - *Viral Tropism
PMC - PMC7232232
OTO - NOTNLM
OT  - BIBD
OT  - VSV
OT  - arenavirus
OT  - pseudotype
OT  - reptarenavirus
OT  - tissue tropism
COIS- The authors declare no conflict of interest.
EDAT- 2020/04/08 06:00
MHDA- 2021/02/20 06:00
PMCR- 2020/04/01
CRDT- 2020/04/08 06:00
PHST- 2020/03/04 00:00 [received]
PHST- 2020/03/29 00:00 [revised]
PHST- 2020/03/31 00:00 [accepted]
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - v12040395 [pii]
AID - viruses-12-00395 [pii]
AID - 10.3390/v12040395 [doi]
PST - epublish
SO  - Viruses. 2020 Apr 2;12(4):395. doi: 10.3390/v12040395.