PMID- 32253738
OWN - NLM
STAT- MEDLINE
DCOM- 20210524
LR  - 20240209
IS  - 1466-1268 (Electronic)
IS  - 1355-8145 (Print)
IS  - 1355-8145 (Linking)
VI  - 25
IP  - 3
DP  - 2020 May
TI  - Involvement of CDKN1A (p21) in cellular senescence in response to heat and 
      irradiation stress during preimplantation development.
PG  - 503-508
LID - 10.1007/s12192-020-01090-4 [doi]
AB  - This study examined the role of cyclin-dependent kinase inhibitor 1a (CDK1A, p21) 
      in response to exogenous stressors during mouse preimplantation embryo 
      development. CDKN1A knockdown (KD) one-cell zygotes were exposed to 39  degrees C heat 
      stress (HS) for 4 days or irradiated by 1 (1-Gy) or 3 (3-Gy) Gy X-rays, and their 
      developmental competence and gene expression were compared with control embryos. 
      CDKN1A KD and HS did not influence early cleavage or subsequent embryonic 
      development; however, HS delayed cavitation and induced elevated Cdkn1a 
      expression in control embryos. Exposure to 1- or 3-Gy had no effect on 
      development to the morula stage; however, a significant number of morulae failed 
      to develop to the blastocyst stage. Interestingly, under the 1-Gy condition, the 
      blastocyst rate of CDKN1A KD embryos (77.7%) was significantly higher than that 
      of the controls (44.4%). In summary, exposure to cellular stressors resulted in 
      the upregulation of Cdkn1a in embryos exposed to HS or X-ray irradiation, 
      particularly in response to heat stress or low-dose X-ray irradiation, and 
      depleting Cdkn1a mRNA alleviated cell cycle arrest. These findings suggest that 
      CDKN1A plays a vital role in cellular senescence during preimplantation embryo 
      development.
FAU - Ock, Sun-A
AU  - Ock SA
AD  - Rural Development Administration, National Institute of Animal Science, 1500 
      Kongjwipatjwi-ro, Isero-myeon, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea.
FAU - Knott, Jason G
AU  - Knott JG
AD  - Developmental Epigenetics Laboratory, Department of Animal Science, Michigan 
      State University, East Lansing, MI, USA. knottj@msu.edu.
AD  - Department of Obstetrics, Gynecology and Reproductive Biology, Michigan State 
      University, East Lansing, MI, USA. knottj@msu.edu.
FAU - Choi, Inchul
AU  - Choi I
AUID- ORCID: 0000-0001-5011-2658
AD  - Division of Animal and Dairy Sciences, College of Agriculture and Life Sciences, 
      Chungnam National University, Daejeon, 34134, Republic of Korea. 
      icchoi@cnu.ac.kr.
LA  - eng
GR  - R01 HD095371/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20200406
PL  - Netherlands
TA  - Cell Stress Chaperones
JT  - Cell stress & chaperones
JID - 9610925
RN  - 0 (Cdkn1a protein, mouse)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Blastocyst/metabolism/radiation effects
MH  - *Cellular Senescence
MH  - Cyclin-Dependent Kinase Inhibitor p21/genetics/*metabolism/physiology
MH  - DNA Damage
MH  - *Embryonic Development/radiation effects
MH  - Gene Expression Regulation, Developmental
MH  - Gene Knockdown Techniques
MH  - Hot Temperature
MH  - Mice
MH  - RNA, Messenger/metabolism
MH  - *Stress, Physiological/genetics
MH  - X-Rays
PMC - PMC7193008
OTO - NOTNLM
OT  - Cdkn1a
OT  - Heat stress
OT  - Irradiation
OT  - Preimplantation
OT  - Senescence
OT  - p21
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/04/08 06:00
MHDA- 2021/05/25 06:00
PMCR- 2020/11/01
CRDT- 2020/04/08 06:00
PHST- 2019/10/01 00:00 [received]
PHST- 2020/03/10 00:00 [accepted]
PHST- 2020/02/15 00:00 [revised]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2021/05/25 06:00 [medline]
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/11/01 00:00 [pmc-release]
AID - S1355-8145(23)01220-8 [pii]
AID - 1090 [pii]
AID - 10.1007/s12192-020-01090-4 [doi]
PST - ppublish
SO  - Cell Stress Chaperones. 2020 May;25(3):503-508. doi: 10.1007/s12192-020-01090-4. 
      Epub 2020 Apr 6.