PMID- 32253822
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1616-5195 (Electronic)
IS  - 1616-5187 (Linking)
VI  - 20
IP  - 6
DP  - 2020 Jun
TI  - Selective Control of Cell Activity with Hydrophilic Polymer-Covered Cationic 
      Nanoparticles.
PG  - e2000049
LID - 10.1002/mabi.202000049 [doi]
AB  - Cationic polymers exhibit high cytotoxicity via strong interaction with cell 
      membranes. To reduce cell membrane damage, a hydrophilic polymer is introduced to 
      the cationic nanoparticle surface. The hydrophilic polymer coating of cationic 
      nanoparticles resulted in a nearly neutral nanoparticle. These particles are 
      applied to mouse fibroblast (3T3) and human cervical adenocarcinoma (Hela) cells. 
      Interestingly, nanoparticles with a long cationic segment decrease cell activity 
      regardless of cell type, while those with a short segment only affect 3T3 cell 
      activity at lower concentrations less than 500 microg mL(-1) . Most nanoparticles are 
      located inside 3T3 cells but on the cell membrane of Hela cells. The short 
      cationic nanoparticle shows negligible cell membrane damage despite its high 
      accumulation on Hela cell membranes. Cell activity changed by hydrophilic 
      polymer-coated cationic nanoparticles is caused by incorporated nanoparticle 
      accumulation in the cells, not cell membrane damage. To suppress the cytotoxicity 
      from the cationic polymer, cationic nanoparticle needs to completely cover with 
      hydrophilic polymer so as not to exhibit the cationic effect and applies to cell 
      with low concentrations to reduce the nonselective cytotoxicity from the cationic 
      polymer.
CI  - (c) 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Lin, Hsiu-Pen
AU  - Lin HP
AD  - Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter 
      Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
AD  - Department of Applied Chemistry, National Chiao Tung University, 1001 University 
      Road, Hsinchu, 300, Taiwan.
FAU - Akimoto, Jun
AU  - Akimoto J
AUID- ORCID: 0000-0003-1554-6869
AD  - Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter 
      Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
AD  - Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 
      Hirosawa, Wako, Saitama, 351-0198, Japan.
FAU - Li, Yaw-Kuen
AU  - Li YK
AD  - Department of Applied Chemistry, National Chiao Tung University, 1001 University 
      Road, Hsinchu, 300, Taiwan.
AD  - Center for Emergent Functional Matter Science, National Chiao Tung University, 
      Hsinchu, 30010, Taiwan.
FAU - Ito, Yoshihiro
AU  - Ito Y
AD  - Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter 
      Science, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
AD  - Nano Medical Engineering Laboratory, RIKEN Cluster for Pioneering Research, 2-1 
      Hirosawa, Wako, Saitama, 351-0198, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200406
PL  - Germany
TA  - Macromol Biosci
JT  - Macromolecular bioscience
JID - 101135941
RN  - 0 (Coated Materials, Biocompatible)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Membrane/*metabolism
MH  - *Coated Materials, Biocompatible/chemistry/pharmacology
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - Nanoparticles/*chemistry
OTO - NOTNLM
OT  - cationic polymers
OT  - cellular membranes
OT  - cytotoxicity
OT  - hydrophilic polymer coating
OT  - nanoparticles
EDAT- 2020/04/08 06:00
MHDA- 2021/06/03 06:00
CRDT- 2020/04/08 06:00
PHST- 2020/02/18 00:00 [received]
PHST- 2020/03/16 00:00 [revised]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/04/08 06:00 [entrez]
AID - 10.1002/mabi.202000049 [doi]
PST - ppublish
SO  - Macromol Biosci. 2020 Jun;20(6):e2000049. doi: 10.1002/mabi.202000049. Epub 2020 
      Apr 6.