PMID- 32253974
OWN - NLM
STAT- MEDLINE
DCOM- 20210607
LR  - 20210607
IS  - 1746-076X (Electronic)
IS  - 1746-0751 (Linking)
VI  - 15
IP  - 3
DP  - 2020 Mar
TI  - Human umbilical cord-mesenchymal stem cells: a promising strategy for corneal 
      epithelial regeneration.
PG  - 1381-1397
LID - 10.2217/rme-2019-0103 [doi]
AB  - Aim: As a strategy to improve the outcome of ex vivo cultivated corneal 
      epithelial transplantation, the role of human umbilical cord-derived mesenchymal 
      stem cells (hUC-MSCs) is investigated in promoting corneal epithelial growth and 
      functions. Materials & methods: Human telomerase-immortalized corneal epithelial 
      cells were characterized and its functions evaluated by scratch migration assay, 
      cellular senescence, HLA expression and spheres formation with hUC-MSC. Results: 
      Expression of corneal epithelial markers was influenced by the duration and 
      method of co-culture. Indirect co-culture improved cellular migration and delayed 
      senescence when treated after 3 and 5 days. hUC-MSC downregulated expression of 
      HLA Class I and II in IFN-gamma-stimulated human telomerase-immortalized corneal 
      epithelial cells. Conclusion: hUC-MSC promote corneal epithelial growth and 
      functions after treatment with hUC-MSC.
FAU - Azmi, Siti Maisura
AU  - Azmi SM
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
FAU - Salih, Mohamed
AU  - Salih M
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
AD  - Faculty of Medical Laboratory Sciences, National University Sudan, Khartoum, 
      Sudan.
FAU - Abdelrazeg, Samar
AU  - Abdelrazeg S
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
AD  - Faculty of Medical Laboratory Sciences, National University Sudan, Khartoum, 
      Sudan.
FAU - Roslan, Fatin Fazrina
AU  - Roslan FF
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
FAU - Mohamed, Rafeezul
AU  - Mohamed R
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
FAU - Tan, Jun Jie
AU  - Tan JJ
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
FAU - Shaharuddin, Bakiah
AU  - Shaharuddin B
AUID- ORCID: 0000-0001-6191-8964
AD  - Regenerative Medicine Cluster, Advanced Medical and Dental Institute, Universiti 
      Sains Malaysia, SAINS@BERTAM, Kepala Batas, Penang 13200, Malaysia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200407
PL  - England
TA  - Regen Med
JT  - Regenerative medicine
JID - 101278116
SB  - IM
EIN - Regen Med. 2020 Jul;15(7):1935. PMID: 32787681
MH  - Cell Differentiation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Corneal Diseases/metabolism/pathology/*therapy
MH  - Epithelium, Corneal/*cytology/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - *Regeneration
MH  - Umbilical Cord/*cytology/metabolism
OTO - NOTNLM
OT  - corneal characterization
OT  - corneal epithelial cells
OT  - ocular surface regeneration
OT  - umbilical cord derived-mesenchymal stem cells
EDAT- 2020/04/08 06:00
MHDA- 2021/06/08 06:00
CRDT- 2020/04/08 06:00
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2021/06/08 06:00 [medline]
PHST- 2020/04/08 06:00 [entrez]
AID - 10.2217/rme-2019-0103 [doi]
PST - ppublish
SO  - Regen Med. 2020 Mar;15(3):1381-1397. doi: 10.2217/rme-2019-0103. Epub 2020 Apr 7.