PMID- 32254333
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200407
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 6
IP  - 19
DP  - 2018 May 21
TI  - Potential application of an injectable hydrogel scaffold loaded with mesenchymal 
      stem cells for treating traumatic brain injury.
PG  - 2982-2992
LID - 10.1039/c7tb03213g [doi]
AB  - In the past few decades, there have been potential applications for stem cell 
      replacement therapy in the treatment of nervous system damage resulting from 
      diseases or traumatic brain injury (TBI). However, the insufficient number of 
      transplanted stem cells and low survival rate caused by a series of negative 
      conditions limit the therapeutic effect. In this contribution, we developed an 
      injectable hydrogel composed of sodium alginate (SA) and hyaluronic acid (HA) as 
      a tissue scaffold to create a more optimal microenvironment for stem cells after 
      implantation. The gelation time of the HA/SA hydrogel exceeded 6 min, which 
      satisfied the requirements for injection performance, and the high ratios of 
      water content and slower degradation speed affirmed that the HA/SA hydrogel is a 
      preferable stem cell scaffold. As a tissue engineering scaffold, the HA/SA 
      hydrogel exhibited appropriately porous structures for stem cell loading and good 
      rheological behavior, which contributed to stem cell differentiation. The in 
      vitro culture experiment proved that the HA/SA scaffold performed well on 
      hUC-MSCs with higher viability ratio and proliferation. Further in vivo tests 
      indicated that the HA/SA scaffold not only protected the injected human umbilical 
      cord mesenchymal stem cells (hUC-MSCs) so that they could maintain a higher 
      survival ratio, but it also contributed to the regeneration of endogenous nerve 
      cells. In summary, this injectable HA/SA hydrogel has the potential to be used 
      for stem cell tissue engineering and support the physiological function recovery 
      of TBI patients.
FAU - Zhang, Kun
AU  - Zhang K
AD  - School of Life Science, Zhengzhou University, 100 Science Road, Zhengzhou 450001, 
      P. R. China. fxguan@126.com.
FAU - Shi, Zhenqing
AU  - Shi Z
FAU - Zhou, Jiankang
AU  - Zhou J
FAU - Xing, Qu
AU  - Xing Q
FAU - Ma, Shanshan
AU  - Ma S
FAU - Li, Qinghua
AU  - Li Q
FAU - Zhang, Yanting
AU  - Zhang Y
FAU - Yao, Minghao
AU  - Yao M
FAU - Wang, Xiaofeng
AU  - Wang X
FAU - Li, Qian
AU  - Li Q
FAU - Li, Jingan
AU  - Li J
FAU - Guan, Fangxia
AU  - Guan F
LA  - eng
PT  - Journal Article
DEP - 20180425
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
SB  - IM
EDAT- 2018/05/21 00:00
MHDA- 2018/05/21 00:01
CRDT- 2020/04/08 06:00
PHST- 2020/04/08 06:00 [entrez]
PHST- 2018/05/21 00:00 [pubmed]
PHST- 2018/05/21 00:01 [medline]
AID - 10.1039/c7tb03213g [doi]
PST - ppublish
SO  - J Mater Chem B. 2018 May 21;6(19):2982-2992. doi: 10.1039/c7tb03213g. Epub 2018 
      Apr 25.