PMID- 32254605
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200407
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 6
IP  - 34
DP  - 2018 Sep 14
TI  - Real-time tracking of the autophagy process in living cells using plasmonically 
      enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles.
PG  - 5460-5465
LID - 10.1039/c8tb01402g [doi]
AB  - To date, a variety of biological assays such as immunostaining, western blotting, 
      enzyme-linked immunosorbent assay (ELISA), and flow cytometry have been used to 
      analyze and trace important biological events and therapies. In addition to these 
      techniques, the application of microscopic analytical techniques such as 
      matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) mass 
      spectrometry and Raman spectroscopy is increasing, allowing information to be 
      obtained at the molecular level. In this study, we have conducted real-time 
      tracking of autophagy, a cellular process that has recently been attracting 
      significant attention. To achieve this purpose, we performed Raman spectroscopy 
      on human oral squamous carcinoma cells (HSC3) incubated with bioactive 
      molecule-modified plasmonic gold nanoparticles. The bioactive 
      molecule-nanoparticle complexes were synthesized using fucoidan, a biopolymer 
      that induces autophagy. By using this platform, it was possible to trace the 
      entire autophagic process successively from cell introduction to autophagic 
      apoptosis. This fusion of nanocomposites and spectroscopic techniques is expected 
      to enable more complex biological processes to be pursued at the molecular level 
      in the future.
FAU - Jang, Hongje
AU  - Jang H
AD  - Laser Dynamics Laboratory, School of Chemistry and Biochemistry, Georgia 
      Institute of Technology, Atlanta, GA30332, USA. melsayed@gatech.edu.
FAU - Kang, Kyungtae
AU  - Kang K
FAU - El-Sayed, Mostafa A
AU  - El-Sayed MA
LA  - eng
PT  - Journal Article
DEP - 20180820
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
SB  - IM
EDAT- 2018/09/14 00:00
MHDA- 2018/09/14 00:01
CRDT- 2020/04/08 06:00
PHST- 2020/04/08 06:00 [entrez]
PHST- 2018/09/14 00:00 [pubmed]
PHST- 2018/09/14 00:01 [medline]
AID - 10.1039/c8tb01402g [doi]
PST - ppublish
SO  - J Mater Chem B. 2018 Sep 14;6(34):5460-5465. doi: 10.1039/c8tb01402g. Epub 2018 
      Aug 20.