PMID- 32255830
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20201001
IS  - 0008-5286 (Print)
IS  - 0008-5286 (Linking)
VI  - 61
IP  - 4
DP  - 2020 Apr
TI  - Field application of a commercial porcine reproductive and respiratory syndrome 
      virus (PRRSV) oral fluid antibody enzyme-linked immunosorbent assay (ELISA).
PG  - 420-423
AB  - A commercial porcine reproductive and respiratory syndrome virus (PRRSV) oral 
      fluid antibody enzyme-linked immunosorbent assay (ELISA) was used on 31 
      commercial swine farms in Ontario using oral fluid samples (~6 per herd) 
      collected from cotton ropes. Using the manufacturer's cutoff [sample-to-positive 
      ratio (S/P) >/= 0.4], 2 of 135 oral fluid samples from 23 PRRSV presumed negative 
      herds tested positive (1.5% false positive rate). Three approaches to improving 
      test diagnostic specificity were compared: i) use a cutoff of S/P >/= 0.8 for 
      individual oral fluid samples; ii) use the current cutoff of S/P >/= 0.4 but use a 
      mean S/P based on several oral fluid samples (6 samples were used in this study); 
      and iii) use serial testing to resolve unexpected positive ELISA results, i.e., 
      retest using a reverse transcription-polymerase chain reaction (RT-PCR) to 
      determine whether low positive S/P ratios are the result of early PRRSV infection 
      in a barn.
CI  - Copyright and/or publishing rights held by the Canadian Veterinary Medical 
      Association.
FAU - Croft, Emily
AU  - Croft E
AD  - Department of Animal Biosciences, University of Guelph, Guelph, Ontario (Croft); 
      Ontario Ministry of Agriculture, Food, and Rural Affairs, 6484 Wellington Road 7, 
      Unit 10, Elora, Ontario N0B 1S0 (Blackwell); Department of Veterinary Diagnostic 
      and Production Animal Medicine, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa 50011, USA (Zimmerman).
FAU - Blackwell, Tim
AU  - Blackwell T
AD  - Department of Animal Biosciences, University of Guelph, Guelph, Ontario (Croft); 
      Ontario Ministry of Agriculture, Food, and Rural Affairs, 6484 Wellington Road 7, 
      Unit 10, Elora, Ontario N0B 1S0 (Blackwell); Department of Veterinary Diagnostic 
      and Production Animal Medicine, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa 50011, USA (Zimmerman).
FAU - Zimmerman, Jeff
AU  - Zimmerman J
AD  - Department of Animal Biosciences, University of Guelph, Guelph, Ontario (Croft); 
      Ontario Ministry of Agriculture, Food, and Rural Affairs, 6484 Wellington Road 7, 
      Unit 10, Elora, Ontario N0B 1S0 (Blackwell); Department of Veterinary Diagnostic 
      and Production Animal Medicine, College of Veterinary Medicine, Iowa State 
      University, Ames, Iowa 50011, USA (Zimmerman).
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Can Vet J
JT  - The Canadian veterinary journal = La revue veterinaire canadienne
JID - 0004653
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Ontario
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Saliva
MH  - Swine
MH  - *Swine Diseases
PMC - PMC7074113
COIS- JZ has served as a consultant to IDEXX laboratories, Inc. on areas separate from 
      this research. The terms of the consulting arrangement have been reviewed and 
      approved by Iowa State University in accordance with its conflict of interest 
      policies.
EDAT- 2020/04/08 06:00
MHDA- 2020/07/14 06:00
PMCR- 2020/10/01
CRDT- 2020/04/08 06:00
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2020/10/01 00:00 [pmc-release]
AID - cvj_04_420 [pii]
PST - ppublish
SO  - Can Vet J. 2020 Apr;61(4):420-423.