PMID- 32256098
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 13
DP  - 2020
TI  - Phocea, Pseudoflavonifractor and Lactobacillus intestinalis: Three Potential 
      Biomarkers of Gut Microbiota That Affect Progression and Complications of 
      Obesity-Induced Type 2 Diabetes Mellitus.
PG  - 835-850
LID - 10.2147/DMSO.S240728 [doi]
AB  - PURPOSE: The purpose of this study was to explore the difference and association 
      between intestinal microbiota and plasma metabolomics between type 2 diabetes 
      mellitus (T2DM) and normal group and to identify potential microbiota biomarkers 
      that contribute the most to the difference in metabolites. METHODS: Six male ZDF 
      model (fa/fa) rats were fed by a Purina #5008 Lab Diet (crude protein 23.5%, 
      crude fat 6.5%) for 3 weeks and their age-matched 6 ZDF control (fa/+) rats were 
      fed by normal rodent diet. Their stool and blood samples were collected at 
      12 weeks. To analyze the microbial populations in these samples, we used a 16S 
      rRNA gene sequencing approach. Liquid chromatography-mass spectrometry (LC-MS) 
      followed by multivariate statistical analysis was applied to the plasma 
      metabolites profiling. Correlation analysis of them was calculated by Pearson 
      statistical method. RESULTS: Twelve potential biomarkers of intestinal microbial 
      flora and 357 differential metabolites were found in ZDF fa/fa rats, among which 
      there are three flora that contributed the most to the perturbation of 
      metabolites, including genus Phocea, Pseudoflavonifractor and species 
      Lactobacillus intestinalis. CONCLUSION: Our study demonstrates the alterations of 
      the abundance and diversity of the intestinal microbiota and the perturbation of 
      metabolites in ZDF rats (fa/fa). We found three potential biomarkers of 
      intestinal microbiota that may lead to perturbation in plasma metabolites. This 
      may prompt new pathogenesis of obesity-related T2DM, but we also need to study 
      further about the causal relationship between intestinal microbe and T2DM, so as 
      to find the target of T2DM treatment or preventive measures.
CI  - (c) 2020 Wang et al.
FAU - Wang, Yuxin
AU  - Wang Y
AUID- ORCID: 0000-0003-2101-413X
AD  - Department of Physical and Chemical Inspection, School of Public Health, Shandong 
      University, Jinan, Shandong 250012, People's Republic of China.
FAU - Ouyang, Meishuo
AU  - Ouyang M
AD  - Department of Public Health, Graduate School of Medicine, Osaka University, 
      Suita, Osaka 565-0871, Japan.
FAU - Gao, Xibao
AU  - Gao X
AD  - Department of Physical and Chemical Inspection, School of Public Health, Shandong 
      University, Jinan, Shandong 250012, People's Republic of China.
FAU - Wang, Shuai
AU  - Wang S
AD  - Institute of Toxicology, School of Public Health, Shandong University, Jinan, 
      Shandong 250012, People's Republic of China.
FAU - Fu, Chunyang
AU  - Fu C
AD  - Department of Physical and Chemical Inspection, School of Public Health, Shandong 
      University, Jinan, Shandong 250012, People's Republic of China.
FAU - Zeng, Jiayi
AU  - Zeng J
AD  - Department of Physical and Chemical Inspection, School of Public Health, Shandong 
      University, Jinan, Shandong 250012, People's Republic of China.
FAU - He, Xiaodong
AU  - He X
AD  - Department of Physical and Chemical Inspection, School of Public Health, Shandong 
      University, Jinan, Shandong 250012, People's Republic of China.
AD  - Shandong Provincial Key Laboratory of Infection and Immunity, School of Basic 
      Medical Sciences, Shandong University, Jinan, Shandong 250012, People's Republic 
      of China.
LA  - eng
PT  - Journal Article
DEP - 20200319
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC7090210
OTO - NOTNLM
OT  - 16S rRNA sequencing
OT  - Zucker diabetic fatty rats
OT  - intestinal microbiota
OT  - plasma metabolomics
OT  - type 2 diabetes mellitus
COIS- Yuxin Wang, Xibao Gao, Chunyang Fu, and Xiaodong He report grants from Ministry 
      of Education of the People's Republic of China and People's Government of 
      Shandong Province during the conduct of the study. The authors report no other 
      conflicts of interest in this work.
EDAT- 2020/04/08 06:00
MHDA- 2020/04/08 06:01
PMCR- 2020/03/19
CRDT- 2020/04/08 06:00
PHST- 2019/12/02 00:00 [received]
PHST- 2020/02/29 00:00 [accepted]
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2020/04/08 06:01 [medline]
PHST- 2020/03/19 00:00 [pmc-release]
AID - 240728 [pii]
AID - 10.2147/DMSO.S240728 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2020 Mar 19;13:835-850. doi: 10.2147/DMSO.S240728. 
      eCollection 2020.