PMID- 32256650
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2020
DP  - 2020
TI  - Immunomodulatory and Antioxidant Activities of a Polysaccharide from Ligustrum 
      vicaryi L. Fruit.
PG  - 5431350
LID - 10.1155/2020/5431350 [doi]
LID - 5431350
AB  - Ligustrum vicaryi L. is a hybrid of Ligustrum ovalifolium Hassk. var. 
      aureo-marginatum and Ligustrum vulgale L., belonging to the Oleaceae family. It 
      is often used as an ornamental shrub due to its golden leaves. However, its 
      medical value is yet to be discovered. Recently, plant polysaccharides have 
      attracted comprehensive attention owing to their biological properties, including 
      immunomodulatory and antioxidant activities. This study aimed to extract, purify, 
      and characterize the polysaccharide from the Ligustrum vicaryi L. fruit and 
      investigate its immunomodulatory and antioxidant activities. The Ligustrum 
      vicaryi L. fruit polysaccharide (LVFP) was obtained by ultrasonic extraction, 
      ethanol precipitation, macroporous resin separation, and dialysis bag 
      purification. The physicochemical properties of the LVFP were elucidated using 
      Fourier-transform infrared spectrometry, high-performance ion chromatography, and 
      high-performance gel filtration chromatography. The results indicated that the 
      LVFP consisted of rhamnose, arabinose, galactose, and glucose in a ratio of 
      1.79 : 7.55 : 4.58 : 1.54, and its molecular weight was 88,949 Da. The 
      immunomodulatory and antioxidant activities of the LVFP were investigated using a 
      cyclophosphamide- (Cy-) induced immunosuppressed mouse model. The results 
      demonstrated that the LVFP significantly increased spleen and thymus indexes, 
      enhanced the phagocytic function of neutrophils, activated B and T lymphocytes, 
      and upregulated serum levels of IL-10 and TNF-alpha. Moreover, we observed that the 
      LVFP relieved Cy-induced liver damage by increasing superoxide dismutase (SOD) 
      and glutathione peroxidase (GSH-px) levels. These results suggested that the LVFP 
      has the immunomodulatory and antioxidant activities, therefore laying a 
      foundation for the application of the LVFP in the pharmaceutical and functional 
      food industries.
CI  - Copyright (c) 2020 Shuling Liu et al.
FAU - Liu, Shuling
AU  - Liu S
AUID- ORCID: 0000-0003-0807-6993
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Wang, Litao
AU  - Wang L
AUID- ORCID: 0000-0002-3734-6676
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Ren, Qiang
AU  - Ren Q
AUID- ORCID: 0000-0002-4728-8676
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Wang, Jianan
AU  - Wang J
AUID- ORCID: 0000-0002-2994-7033
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Li, Yanzhi
AU  - Li Y
AUID- ORCID: 0000-0001-7687-7114
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Wang, Guanghui
AU  - Wang G
AUID- ORCID: 0000-0002-0817-3163
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Gao, Huijie
AU  - Gao H
AUID- ORCID: 0000-0001-5473-8199
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Du, Rui
AU  - Du R
AUID- ORCID: 0000-0003-3397-3645
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
FAU - Qin, Wei
AU  - Qin W
AUID- ORCID: 0000-0003-2161-387X
AD  - School of Pharmacy, Jining Medical University, Rizhao 276800, China.
LA  - eng
PT  - Journal Article
DEP - 20200317
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC7103051
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2020/04/08 06:00
MHDA- 2020/04/08 06:01
PMCR- 2020/03/17
CRDT- 2020/04/08 06:00
PHST- 2019/07/05 00:00 [received]
PHST- 2019/12/09 00:00 [revised]
PHST- 2020/02/20 00:00 [accepted]
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2020/04/08 06:01 [medline]
PHST- 2020/03/17 00:00 [pmc-release]
AID - 10.1155/2020/5431350 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2020 Mar 17;2020:5431350. doi: 
      10.1155/2020/5431350. eCollection 2020.