PMID- 32256720
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 19
IP  - 4
DP  - 2020 Apr
TI  - Effects of propofol on LC3II and mTOR/p-mTOR expression during 
      ischemia-reperfusion myocardium injury in rats with type 2 diabetes mellitus.
PG  - 2441-2448
LID - 10.3892/etm.2020.8499 [doi]
AB  - To investigate the effects of propofol on myocardial ischemia reperfusion in rats 
      with type 2 diabetes, male adult rats were divided into five groups: 
      Sham-operation (CC), ischemia-reperfusion (CI), low-dose propofol (LP), 
      moderate-dose propofol (MP) and high-dose propofol (HP). The LP, MP and HP groups 
      were administered with 6, 12 and 24 mg/kg/h propofol, respectively, prior to 
      occlusion. Heart rate (HR), left ventricular systolic pressure (LVSP) and the 
      rate (dp/dt max) of left ventricular pressure rise in early systole (+/-dp/dt max) 
      were recorded. The role of autophagy was also studied by measuring the levels of 
      superoxide dismutase (SOD), malondialdehyde (MDA), autophagy marker protein 
      LC3II, mammalian target of rapamycin (mTOR)/phosphorylate (p)-mTOR and cardiac 
      troponin T (cTnT). The myocardial morphological features were assessed using 
      light and electron microscopy. The present results demonstrated that the HR, 
      LVSP, +dp/dt and -dp/dt levels in the propofol groups (LP, MP and HP) were 
      significantly increased (P<0.05) when compared with the CI group. The myocardial 
      cells in the MP group showed mild edematous changes and partially dissolved 
      mitochondrial cristae and membrane rupture. SOD, cTnT and MDA levels were 
      significantly decreased (P<0.05), mTOR expression decreased significantly 
      (P<0.05) and p-mTOR expression increased significantly in the MP group (P<0.05). 
      The present study demonstrated the protective effects of propofol in T2DM rats 
      exhibiting MIRI, with an optimal protective effect at an infusion rate of 12 
      mg/kg/h. Additionally, the results revealed that propofol led to significant 
      reductions in LC3II and mTOR serum levels and the inhibition of autophagy in 
      myocardial cells.
CI  - Copyright: (c) Wang et al.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Anesthesiology, Affiliated Hospital of Hebei University, Baoding, 
      Hebei 071000, P.R. China.
FAU - Zhang, Kecheng
AU  - Zhang K
AD  - Department of Medicine, Hebei University, Baoding, Hebei 071000, P.R. China.
FAU - Qi, Xiuru
AU  - Qi X
AD  - Department of Medicine, Hebei University, Baoding, Hebei 071000, P.R. China.
FAU - Yang, Guang
AU  - Yang G
AD  - Department of Anesthesiology, Affiliated Hospital of Hebei University, Baoding, 
      Hebei 071000, P.R. China.
FAU - Wang, Hongjie
AU  - Wang H
AD  - Department of Medicine, Hebei University, Baoding, Hebei 071000, P.R. China.
FAU - Zhang, Zhe
AU  - Zhang Z
AD  - Hebei Medical Science and Technology Development Research Center, Shijiazhuang, 
      Hebei 051000, P.R. China.
FAU - Yang, Baofeng
AU  - Yang B
AD  - Health and Family Planning Commission of Hebei, Shijiazhuang, Hebei 050000, P.R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200207
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7098214
OTO - NOTNLM
OT  - LC3II
OT  - autophagy
OT  - cardiac troponin T
OT  - diabetes mellitus
OT  - malondialdehyde
OT  - mechanistic target of rapamycin
OT  - myocardial ischemia reperfusion
OT  - phospho- mechanistic target of rapamycin
OT  - propofol
OT  - superoxide dismutase
EDAT- 2020/04/08 06:00
MHDA- 2020/04/08 06:01
PMCR- 2020/02/07
CRDT- 2020/04/08 06:00
PHST- 2019/02/13 00:00 [received]
PHST- 2019/11/05 00:00 [accepted]
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2020/04/08 06:01 [medline]
PHST- 2020/02/07 00:00 [pmc-release]
AID - ETM-0-0-8499 [pii]
AID - 10.3892/etm.2020.8499 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Apr;19(4):2441-2448. doi: 10.3892/etm.2020.8499. Epub 2020 Feb 
      7.