PMID- 32257539
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2152-5250 (Print)
IS  - 2152-5250 (Electronic)
IS  - 2152-5250 (Linking)
VI  - 11
IP  - 2
DP  - 2020 Apr
TI  - Divergent Effect of Tacalcitol (PRI-2191) on Th17 Cells in 4T1 Tumor Bearing 
      Young and Old Ovariectomized Mice.
PG  - 241-253
LID - 10.14336/AD.2019.0618 [doi]
AB  - Vitamin D and its analogs are known for their role in the development of breast 
      cancer and in immunomodulation. Our previous studies have shown the 
      pro-metastatic effect of calcitriol and tacalcitol (PRI-2191) in young mice 
      bearing 4T1 breast cancer and the anti-metastatic effect in aged ovariectomized 
      (OVX) mice. Therefore, the aim of our work was to characterize Th17 cell 
      population in young and aged OVX mice bearing 4T1 tumors treated with calcitriol 
      and PRI-2191. The expression of genes typical for Th17 cells was examined in 
      splenocytes, as well as splenocytes differentiated with IL-6 and TGF-beta to Th17 
      cells (iTh17). Expression of genes encoding vitamin D receptor (Vdr) and 
      osteopontin (Spp1) as well as the secretion of IL-17A were evaluated in iTh17 
      cells. PRI-2191 treatment increased the expression of Rora and Rorc transcription 
      factors, Il17a, Il17re and Il21 in iTh17 cells from young mice. In aged OVX mice 
      this effect was not observed. Increased expression was observed in the case of 
      Vdr and Spp1 genes in iTh17 cells from young mice treated with PRI-2191. What is 
      more, in young mice treated with PRI-2191 the secretion of IL-17A to the culture 
      media by iTh17 cells was increased, whereas in aged OVX mice a significant 
      decrease was noted. Increased expression of Spp1 in young mice treated with 
      PRI-2191 may enhance the differentiation of Th17 cells.
CI  - Copyright: (c) 2020 Pawlik et al.
FAU - Pawlik, Agata
AU  - Pawlik A
AD  - 1Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Anisiewicz, Artur
AU  - Anisiewicz A
AD  - 1Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Filip-Psurska, Beata
AU  - Filip-Psurska B
AD  - 1Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Klopotowska, Dagmara
AU  - Klopotowska D
AD  - 1Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Maciejewska, Magdalena
AU  - Maciejewska M
AD  - 1Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Mazur, Andrzej
AU  - Mazur A
AD  - 2Universite Clermont Auvergne, INRA, UNH, Unite de Nutrition Humaine, F-63000 
      Clermont-Ferrand, France.
FAU - Wietrzyk, Joanna
AU  - Wietrzyk J
AD  - 1Department of Experimental Oncology, Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20200309
PL  - United States
TA  - Aging Dis
JT  - Aging and disease
JID - 101540533
PMC - PMC7069462
OTO - NOTNLM
OT  - IL-17A
OT  - PRI-2191
OT  - Th17
OT  - breast cancer
OT  - calcitriol
OT  - metastasis
EDAT- 2020/04/08 06:00
MHDA- 2020/04/08 06:01
PMCR- 2020/04/01
CRDT- 2020/04/08 06:00
PHST- 2019/04/06 00:00 [received]
PHST- 2019/06/18 00:00 [accepted]
PHST- 2020/04/08 06:00 [entrez]
PHST- 2020/04/08 06:00 [pubmed]
PHST- 2020/04/08 06:01 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - ad-11-2-241 [pii]
AID - 10.14336/AD.2019.0618 [doi]
PST - epublish
SO  - Aging Dis. 2020 Mar 9;11(2):241-253. doi: 10.14336/AD.2019.0618. eCollection 2020 
      Apr.