PMID- 32261943
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240102
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 3
IP  - 2
DP  - 2015 Jan 14
TI  - Enhanced DNA release from disulfide-containing layered nanocomplexes by 
      heparin-electrostatic competition.
PG  - 225-237
LID - 10.1039/c4tb01113a [doi]
AB  - Polycationic vectors are often used to deliver DNA for cancer therapies, but 
      their inefficiency in releasing DNA from the polyplexes after endosomal escape 
      limits DNA transcription and their efficient application in vivo. In this study, 
      DNA/PEI polyplexes were cross-linked by a reduction-sensitive disulfide bond and 
      then further complexed with electrostatic competitive heparin (HP) and 
      hyaluronidase (HAase)-sensitive hyaluronate (HA) to obtain DNA/PEIS/HA-HP 
      (DPSHA-HP). DPSHA-HP was stable in an extracellular environment (pH = 7.4) and 
      degraded by HAase after targeted HA receptor CD44-mediated cell endocytosis, 
      causing the outer shielding of the nanocomplex to loosen. The resulting partially 
      exposed disulfide-linked DNA/PEI nanocomplexes efficiently ruptured the endosome, 
      facilitating the cleavage of disulfide bonds and the release of DNA/PEI 
      polyplexes into the cytoplasm, where DNA release from the polyplexes was 
      remarkably enhanced due to strong electrostatic competition of HP with PEI. 
      Consequently, DPSHA-HP exhibited excellent DNA transfection of the target cells, 
      better than disulfide cross-linked DNA/PEI (25 kDa) and DNA/PEIS/HA. Moreover, 
      these novel layered nanocomplexes have high efficiency in down-regulating 
      B-cell-specific Moloney murine leukemia virus insertion site 1 (Bmi-1) and 
      exhibit significant inhibition of tumor formation with minimal toxicity in a 
      mouse tumor model.
FAU - Chen, Zhenzhen
AU  - Chen Z
AD  - State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous 
      Metals Chemistry and Resources Utilization of Gansu Province, College of 
      Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China. 
      lfzhang@lzu.edu.cn.
FAU - He, Yuling
AU  - He Y
FAU - Zhang, Lifen
AU  - Zhang L
FAU - Li, Yanfeng
AU  - Li Y
LA  - eng
PT  - Journal Article
DEP - 20141110
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
SB  - IM
EIN - J Mater Chem B. 2024 Jan 17;12(3):828-829. PMID: 38163996
EDAT- 2015/01/14 00:00
MHDA- 2015/01/14 00:01
CRDT- 2020/04/09 06:00
PHST- 2020/04/09 06:00 [entrez]
PHST- 2015/01/14 00:00 [pubmed]
PHST- 2015/01/14 00:01 [medline]
AID - 10.1039/c4tb01113a [doi]
PST - ppublish
SO  - J Mater Chem B. 2015 Jan 14;3(2):225-237. doi: 10.1039/c4tb01113a. Epub 2014 Nov 
      10.