PMID- 32262129
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200408
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 3
IP  - 12
DP  - 2015 Mar 28
TI  - Encapsulation of VEGF(165) into magnetic PLGA nanocapsules for potential local 
      delivery and bioactivity in human brain endothelial cells.
PG  - 2538-2544
LID - 10.1039/c4tb01895h [doi]
AB  - Angiogenesis is an important repairing mechanism in response to ischemia. The 
      administration of pro-angiogenic proteins is an attractive therapeutic strategy 
      to enhance angiogenesis after an ischemic event. Their labile structures and 
      short circulation times in vivo are the main obstacles that reduce the 
      bioactivity and dosage of such proteins at the target site. We report on 
      poly(d,l-lactic-co-glycolic acid) (PLGA) nanocapsules (diameter < 200 nm) 
      containing bioactive vascular endothelial growth factor-165 (VEGF(165)) in the 
      inner core and superparamagnetic iron oxide nanoparticles (SPIONs) embedded in 
      the polymeric shell. The system showed good encapsulation efficiencies for both 
      VEGF(165) and SPIONs and a sustained protein release over 14 days. In vitro 
      studies confirmed protein bioactivity in the form of significantly increased 
      proliferation in human microvascular brain endothelial cell cultures once the 
      protein was released. Through magnetic resonance imaging (MRI) measurements we 
      demonstrated excellent T(2) contrast image properties with r(2) values as high as 
      213 mM(-1) s(-1). In addition, magnetic VEGF(165)-loaded PLGA nanocapsules could 
      be displaced and accumulated under an external magnetic field for guiding and 
      retention purposes. We therefore suggest that using VEGF(165)-loaded magnetic 
      PLGA nanocapsules may become a new targeted protein-delivery strategy in the 
      development of future pro-angiogenic treatments, as for instance those directed 
      to neurorepair after an ischemic event.
FAU - Carenza, E
AU  - Carenza E
AD  - Institut de Ciencia de Materials de Barcelona, Consejo Superior de 
      Investigaciones Cientificas (ICMAB-CSIC), Campus de la UAB, 08193 
      Bellaterra(Barcelona), Catalunya, Spain. roig@icmab.es.
FAU - Jordan, O
AU  - Jordan O
FAU - Martinez-San Segundo, P
AU  - Martinez-San Segundo P
FAU - Jirik, R
AU  - Jirik R
FAU - Starcuk Jr, Z
AU  - Starcuk Jr Z
FAU - Borchard, G
AU  - Borchard G
FAU - Rosell, A
AU  - Rosell A
FAU - Roig, A
AU  - Roig A
LA  - eng
PT  - Journal Article
DEP - 20150223
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
SB  - IM
EDAT- 2015/03/28 00:00
MHDA- 2015/03/28 00:01
CRDT- 2020/04/09 06:00
PHST- 2020/04/09 06:00 [entrez]
PHST- 2015/03/28 00:00 [pubmed]
PHST- 2015/03/28 00:01 [medline]
AID - 10.1039/c4tb01895h [doi]
PST - ppublish
SO  - J Mater Chem B. 2015 Mar 28;3(12):2538-2544. doi: 10.1039/c4tb01895h. Epub 2015 
      Feb 23.