PMID- 32263500
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200408
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 4
IP  - 36
DP  - 2016 Sep 28
TI  - Enhanced photoelectrochemical aptasensing platform amplified through the 
      sensitization effect of CdTe@CdS core-shell quantum dots coupled with 
      exonuclease-I assisted target recycling.
PG  - 6117-6124
LID - 10.1039/c6tb01807f [doi]
AB  - A novel, enhanced photoelectrochemical aptasensing platform was developed through 
      integrating the sensitization effect of CdTe@CdS core-shell quantum dots (QDs) 
      coupled with exonuclease-I (Exo-I) assisted target recycling for significant 
      signal amplification. Carcinoembryonic antigen (CEA) was selected as the target 
      analyte to exhibit the analytical performance of this platform. Specifically, 
      nitrogen-doped mesoporous TiO(2) (mTiO(2):N) was firstly synthesized through an 
      evaporation-induced self-assembly (EISA) method. Then, an mTiO(2):N/Au hybrid 
      structure was prepared through depositing Au nanoparticles on the surface of the 
      mTiO(2):N film and this acted as the photoelectrochemical matrix to immobilize 
      the complementary DNA (cDNA) of the CEA aptamer probe (pDNA). CdTe@CdS core-shell 
      QDs as sensitization agents were covalently bound at the front-end of pDNA. After 
      pDNA was hybridized with cDNA, the labels of the CdTe@CdS core-shell QDs were 
      very close to the mTiO(2):N/Au electrode surface, resulting in an evidently 
      enhanced photocurrent intensity due to the generation of the sensitization 
      effect. When the aptasensor was incubated with CEA and Exo-I simultaneously, 
      CdTe@CdS QD labeled pDNA (denoted QD-pDNA) became specifically bound with CEA and 
      meanwhile was separated from the electrode surface, leading to an obviously 
      weakened sensitization effect and a decreased photocurrent intensity. Moreover, 
      as Exo-I could digest the single strand form of pDNA, the previously bound CEA 
      was released and continuously interacted with the rest of the pDNA on the 
      electrode surface, causing further decreased photocurrent intensity. The 
      well-designed photoelectrochemical aptasensor exhibited a low detection limit of 
      0.12 pg mL(-1) and a wide linear range from 0.5 pg mL(-1) to 10 ng mL(-1) for CEA 
      detection, and it also showed good selectivity, reproducibility and stability. 
      The proposed signal amplification strategy provides a promising universal 
      photoelectrochemical platform for sensitively detecting various biomolecules at 
      low levels.
FAU - Cong, Xinxin
AU  - Cong X
AD  - College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal 
      University, Jinan, 250014, P. R. China. wangxl@sdnu.edu.cn.
FAU - Fan, Gao-Chao
AU  - Fan GC
FAU - Wang, Xiaolei
AU  - Wang X
FAU - Abdel-Halim, E S
AU  - Abdel-Halim ES
FAU - Zhu, Jun-Jie
AU  - Zhu JJ
LA  - eng
PT  - Journal Article
DEP - 20160901
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
SB  - IM
EDAT- 2016/09/28 00:00
MHDA- 2016/09/28 00:01
CRDT- 2020/04/09 06:00
PHST- 2020/04/09 06:00 [entrez]
PHST- 2016/09/28 00:00 [pubmed]
PHST- 2016/09/28 00:01 [medline]
AID - 10.1039/c6tb01807f [doi]
PST - ppublish
SO  - J Mater Chem B. 2016 Sep 28;4(36):6117-6124. doi: 10.1039/c6tb01807f. Epub 2016 
      Sep 1.