PMID- 32264356
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200408
IS  - 2050-7518 (Electronic)
IS  - 2050-750X (Linking)
VI  - 5
IP  - 30
DP  - 2017 Aug 14
TI  - A novel biomimetic scaffold with hUCMSCs for lumbar fusion.
PG  - 5996-6007
LID - 10.1039/c6tb02640k [doi]
AB  - Discectomy and lumbar fusion are common clinical approaches to treating 
      intervertebral disc (IVD) degeneration with the aid of autologous bone and/or 
      biomaterials. Biomaterials are considered suitable if they are biodegradable and 
      can guide tissue regeneration. These features are necessary for total IVD 
      removal, when it degenerates, as lumbar fusion treatment, avoiding secondary 
      damage caused by the use of autogenous bone. In this work, a novel biomimetic 
      porous chitosan/poly(l-lactic acid) scaffold with human umbilical cord 
      mesenchymal stem cells (hUCMSCs) was applied in lumbar fusion. Hierarchically 
      porous chitosan scaffolds were prepared using molds, porogens and freeze drying, 
      and then poly(l-lactic acid) networks were distributed throughout the interior 
      scaffold to enhance mechanical strength. We conducted cell culture in vitro and 
      animal experiments in vivo. The scaffolds were incubated in hUCMSCs medium and 
      co-cultured for 8 days, and then implanted into half-destroyed IVDs in New 
      Zealand rabbits. The results show that scaffolds with hUCMSCs had greater ability 
      to guide disc regeneration than the blank control and autologous bone, as 
      determined using X-rays, computed tomography, and histologic analyses. Findings 
      confirmed the role of hUCMSCs stimulation in bone-tissue healing and IVD 
      regeneration. This hMUMSCs-based approach, together with the strategy proposed 
      for incorporating osteoblastic cells into scaffolds that promote endogenous or 
      synthetic repair mechanisms, may then be used to develop strategies for the 
      stem-cell-based healing of other acute injuries and chronic diseases.
FAU - Li, Na
AU  - Li N
AD  - Department of Materials Science and Engineering, Jinan University, Guangzhou, 
      510632, China. tlihuali@jnu.edu.cn tcrz9@jnu.edu.cn.
FAU - Li, Zhiwen
AU  - Li Z
FAU - Li, Riwang
AU  - Li R
FAU - Tian, Jinhuan
AU  - Tian J
FAU - Sun, Guodong
AU  - Sun G
FAU - Li, Lihua
AU  - Li L
FAU - Wu, Di
AU  - Wu D
FAU - Ding, Shan
AU  - Ding S
FAU - Zhou, Changren
AU  - Zhou C
LA  - eng
PT  - Journal Article
DEP - 20170717
PL  - England
TA  - J Mater Chem B
JT  - Journal of materials chemistry. B
JID - 101598493
SB  - IM
EDAT- 2017/08/14 00:00
MHDA- 2017/08/14 00:01
CRDT- 2020/04/09 06:00
PHST- 2020/04/09 06:00 [entrez]
PHST- 2017/08/14 00:00 [pubmed]
PHST- 2017/08/14 00:01 [medline]
AID - 10.1039/c6tb02640k [doi]
PST - ppublish
SO  - J Mater Chem B. 2017 Aug 14;5(30):5996-6007. doi: 10.1039/c6tb02640k. Epub 2017 
      Jul 17.