PMID- 32265644
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 14
DP  - 2020
TI  - Nix Plays a Neuroprotective Role in Early Brain Injury After Experimental 
      Subarachnoid Hemorrhage in Rats.
PG  - 245
LID - 10.3389/fnins.2020.00245 [doi]
LID - 245
AB  - Nix is located in the outer membrane of mitochondria, mediates mitochondrial 
      fission and implicated in many neurological diseases. However, the association 
      between Nix and subarachnoid hemorrhage (SAH) has not previously been reported. 
      Therefore, the present study was designed to evaluate the expression of Nix and 
      its role in early brain injury (EBI) after SAH. Adult male Sprague-Dawley (SD) 
      rats were randomly assigned to various time points for investigation after SAH. A 
      rat model of SAH was induced by injecting 0.3 ml of autologous non-heparinized 
      arterial blood into the prechiasmatic cistern. The expression of Nix was 
      investigated by Western blot and immunohistochemistry. Next, Nix-specific 
      overexpression plasmids and small interfering RNAs (siRNAs) were separately 
      administered. Western blot, neurological scoring, Morris water maze, terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining and 
      fluoro-jade B (FJB) staining were performed to evaluate the role of Nix in EBI 
      following SAH. We found that Nix was expressed in neurons and its expression 
      level in the SAH groups was higher than that in the Sham group, which peaked at 
      24 h after SAH. Overexpression of Nix following SAH significantly decreased the 
      expression of translocase of outer mitochondrial membrane 20 (TOMM20, a marker of 
      mitochondria), ameliorated neurological/cognitive deficits induced by SAH, and 
      reduced the total number of apoptotic/neurodegenerative cells, whereas siRNA 
      knockdown of Nix yielded opposite effects. Taken together, our findings 
      demonstrated that the expression of Nix is increased in neurons after 
      experimental SAH in rats, and may play a neuroprotective role in EBI following 
      SAH.
CI  - Copyright (c) 2020 Zhang, Yuan, Liang, Pan, Li, Li, Shen, Wang and Chen.
FAU - Zhang, Juyi
AU  - Zhang J
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Yuan, Guiqiang
AU  - Yuan G
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Liang, Tianyu
AU  - Liang T
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Pan, Pengjie
AU  - Pan P
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Li, Xiang
AU  - Li X
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Li, Haiying
AU  - Li H
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Shen, Haitao
AU  - Shen H
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Wang, Zhong
AU  - Wang Z
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
FAU - Chen, Gang
AU  - Chen G
AD  - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First 
      Affiliated Hospital of Soochow University, Suzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200324
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC7108665
OTO - NOTNLM
OT  - Nix
OT  - TOMM20
OT  - early brain injury
OT  - neuroprotection
OT  - subarachnoid hemorrhage
EDAT- 2020/04/09 06:00
MHDA- 2020/04/09 06:01
PMCR- 2020/01/01
CRDT- 2020/04/09 06:00
PHST- 2019/11/12 00:00 [received]
PHST- 2020/03/04 00:00 [accepted]
PHST- 2020/04/09 06:00 [entrez]
PHST- 2020/04/09 06:00 [pubmed]
PHST- 2020/04/09 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2020.00245 [doi]
PST - epublish
SO  - Front Neurosci. 2020 Mar 24;14:245. doi: 10.3389/fnins.2020.00245. eCollection 
      2020.