PMID- 32266093
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2156-6976 (Print)
IS  - 2156-6976 (Electronic)
IS  - 2156-6976 (Linking)
VI  - 10
IP  - 3
DP  - 2020
TI  - alpha1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of 
      cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in 
      non-small cell lung cancer (NSCLC).
PG  - 816-837
AB  - Cancer-associated fibroblasts (CAFs) are the main cancer-promoting component in 
      the tumor microenvironment (TME) of non-small cell lung cancer (NSCLC). 
      alpha1,6-Fucosyltransferase (FUT8), the key enzyme catalyzing core alpha1,6-fucosylation 
      (CF), plays a promoting role in multiple malignancies. In the current study, we 
      investigated the function of FUT8 in CAFs and elucidated the mechanism through 
      which FUT8 regulates the cancer-promoting capacity of CAFs in NSCLC. A 
      bioinformatics analysis was performed to reveal the relationship between FUT8 and 
      CAFs. Resected specimens from NSCLC patients were analyzed to assess the 
      expression of FUT8 in CAFs. Primary CAFs and normal lung fibroblasts (NLFs) were 
      extracted from NSCLC patient specimens and were co-cultured with NSCLC cell lines 
      in a novel 3D-printed non-contact co-culture device. An In vivo CAF/NSCLC 
      co-injection tumorigenesis assay was performed using nude mice to study the 
      function of FUT8/CF in TME formation. The current study revealed that 
      FUT8-mediated CF in CAFs plays a positive role in the cancer-promoting capacity 
      of these cells. FUT8 overexpression was observed in CAFs isolated from some lung 
      adenocarcinoma cases. Further investigation showed that FUT8/CF in CAFs promoted 
      the formation of an invasive and malignant TME in vivo and in vitro, and the 
      resulting NSCLC cells exhibited faster proliferation and increased invasiveness. 
      EGFR signaling exerts a catalytic effect on the cancer-promoting capacity of CAFs 
      and is regulated by the CF modification of the EGFR protein.
CI  - AJCR Copyright (c) 2020.
FAU - Li, Fengzhou
AU  - Li F
AD  - The First Affiliated Hospital, Dalian Medical University Dalian, China.
FAU - Zhao, Shilei
AU  - Zhao S
AD  - The First Affiliated Hospital, Dalian Medical University Dalian, China.
FAU - Cui, Yanwei
AU  - Cui Y
AD  - Zhongshan Hospital, Dalian University Dalian, China.
FAU - Guo, Tao
AU  - Guo T
AD  - The First Affiliated Hospital, Dalian Medical University Dalian, China.
FAU - Qiang, Jiaqi
AU  - Qiang J
AD  - Dalian Municipal Central Hospital Affiliated to Dalian Medical University Dalian, 
      China.
FAU - Xie, Qiang
AU  - Xie Q
AD  - The First Affiliated Hospital, Dalian Medical University Dalian, China.
FAU - Yu, Wendan
AU  - Yu W
AD  - Institute of Cancer Stem Cell, Dalian Medical University Dalian, China.
FAU - Guo, Wei
AU  - Guo W
AD  - Institute of Cancer Stem Cell, Dalian Medical University Dalian, China.
FAU - Deng, Wuguo
AU  - Deng W
AD  - Sun Yat-sen University Cancer Center, State Key Lab of Oncology in South China 
      Guangzhou, China.
FAU - Gu, Chundong
AU  - Gu C
AD  - The First Affiliated Hospital, Dalian Medical University Dalian, China.
FAU - Wu, Taihua
AU  - Wu T
AD  - The First Affiliated Hospital, Dalian Medical University Dalian, China.
LA  - eng
PT  - Journal Article
DEP - 20200301
PL  - United States
TA  - Am J Cancer Res
JT  - American journal of cancer research
JID - 101549944
PMC - PMC7136908
OTO - NOTNLM
OT  - Cancer-associated fibroblasts
OT  - epidermal growth factor receptor
OT  - non-small cell lung cancer
OT  - tumor microenvironment
OT  - alpha1,6-fucosyltransferase
COIS- None.
EDAT- 2020/04/09 06:00
MHDA- 2020/04/09 06:01
PMCR- 2020/03/01
CRDT- 2020/04/09 06:00
PHST- 2019/01/26 00:00 [received]
PHST- 2020/02/28 00:00 [accepted]
PHST- 2020/04/09 06:00 [entrez]
PHST- 2020/04/09 06:00 [pubmed]
PHST- 2020/04/09 06:01 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
PST - epublish
SO  - Am J Cancer Res. 2020 Mar 1;10(3):816-837. eCollection 2020.