PMID- 32271395
OWN - NLM
STAT- MEDLINE
DCOM- 20210315
LR  - 20210315
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 6
DP  - 2020 Mar
TI  - Protective effect of magnesium sulfate on cranial nerves in preeclampsia rats 
      through NF-kappaB/ICAM-1 pathway.
PG  - 2785-2794
LID - 20639 [pii]
LID - 10.26355/eurrev_202003_20639 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate the protective effect of 
      magnesium sulfate (MgSO4) on the cranial nerves of preeclampsia (PE) rats through 
      the nuclear factor-kappaB (NF-kappaB)/intercellular adhesion molecule-1 (ICAM-1) pathway. 
      MATERIALS AND METHODS: A total of 30 pregnant rats were randomly divided into 
      three groups, including control group, model group, and treatment group, with 10 
      rats in each group. Systolic blood pressure was measured at 13 d, 15 d, and 19 d. 
      The apoptosis level in brain tissues was detected via Western blotting and 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      assay. Protein expression of genes was detected using immunohistochemical 
      staining. Moreover, the messenger ribonucleic acid (mRNA) expressions of NF-kappaB 
      and ICAM-1 in brain tissues were determined through Reverse 
      Transcription-Polymerase Chain Reaction (RT-PCR). RESULTS: Systolic blood 
      pressure exhibited significant differences among the three groups at 15 d and 19 
      d of gestational age (p<0.05). At 15 d of gestational age, systolic blood 
      pressure was significantly higher in model group than that of control group 
      (p<0.05). However, it was slightly lower in treatment group than model group 
      (p<0.05). At 19 d of gestational age, systolic blood pressure was significantly 
      higher in model group than control group (p<0.05). However, it decreased 
      remarkably in treatment group when compared with model group (p<0.05). In 
      treatment group, systolic blood pressure at 19 d was significantly lower than 
      that at 15 d (p<0.05). Subsequent Western blotting revealed that the protein 
      expression of B-cell lymphoma-2 (Bcl-2) in brain tissues decreased evidently, 
      whereas the expression of Bcl-2 associated X protein (Bax) increased 
      significantly in model group compared with control group, showing statistically 
      significant differences (p<0.01). The protein expression of Bcl-2 in brain 
      tissues increased significantly, while the expression of Bax declined remarkably 
      in treatment group compared with model group (p<0.01). The number of apoptotic 
      cells in model group and treatment group increased significantly compared with 
      that in control group, with the largest in model group (p<0.05). However, it 
      remarkably declined in treatment group compared with model group (p<0.05). These 
      results suggested that MgSO4 treatment could significantly reduce neuronal 
      apoptosis in PE rats. According to the results of immunohistochemistry, the 
      protein expressions of NF-kappaB and ICAM-1 in brain tissues were significantly 
      higher in model group and treatment group than those in control group (p<0.05). 
      However, they were significantly lower in treatment group than model group 
      (p<0.05). RT-PCR results manifested that the mRNA expressions of NF-kappaB and ICAM-1 
      in brain tissues exhibited evident differences among the three groups (p<0.05). 
      Model group and treatment group showed significantly up-regulated mRNA 
      expressions of NF-kappaB and ICAM-1 in brain tissues compared with control group 
      (p<0.05). The highest mRNA expression was observed in model group. However, 
      treatment group exhibited remarkably decreased mRNA expressions of NF-kappaB and 
      ICAM-1 in brain tissues compared with model group (p<0.05). CONCLUSIONS: MgSO4 
      exerts a protective effect on cranial nerves of PE rats by inhibiting the 
      NF-kappaB/ICAM-1 signaling pathway.
FAU - Shi, C-X
AU  - Shi CX
AD  - Department of Obstetrics, Zhangqiu Maternal and Child Health Hospital, Zhangqiu, 
      China. 15064127221@163.com.
FAU - Qi, Q-H
AU  - Qi QH
FAU - Xu, J
AU  - Xu J
FAU - Zhao, W-W
AU  - Zhao WW
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (ICAM1 protein, rat)
RN  - 0 (NF-kappa B)
RN  - 0 (Protective Agents)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 7487-88-9 (Magnesium Sulfate)
SB  - IM
MH  - Animals
MH  - Blood Pressure/drug effects
MH  - Cranial Nerves/*drug effects/metabolism
MH  - Disease Models, Animal
MH  - Female
MH  - Intercellular Adhesion Molecule-1/*metabolism
MH  - Magnesium Sulfate/pharmacology
MH  - NF-kappa B/*metabolism
MH  - Pre-Eclampsia
MH  - Pregnancy
MH  - Protective Agents
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2020/04/10 06:00
MHDA- 2021/03/16 06:00
CRDT- 2020/04/10 06:00
PHST- 2020/04/10 06:00 [entrez]
PHST- 2020/04/10 06:00 [pubmed]
PHST- 2021/03/16 06:00 [medline]
AID - 20639 [pii]
AID - 10.26355/eurrev_202003_20639 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Mar;24(6):2785-2794. doi: 
      10.26355/eurrev_202003_20639.