PMID- 32271805
OWN - NLM
STAT- MEDLINE
DCOM- 20200713
LR  - 20231113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 15
IP  - 4
DP  - 2020
TI  - Small G protein signaling modulator 3 (SGSM3) knockdown attenuates apoptosis and 
      cardiogenic differentiation in rat mesenchymal stem cells exposed to hypoxia.
PG  - e0231272
LID - 10.1371/journal.pone.0231272 [doi]
LID - e0231272
AB  - Connexin 43 (Cx43) may be important in cell death and survival due to 
      cell-to-cell communication-independent mechanisms. In our previous study, we 
      found that small G protein signaling modulator 3 (SGSM3), a partner of Cx43, 
      contributes to myocardial infarction (MI) in rat hearts. Based on these previous 
      results, we hypothesized that SGSM3 could also play a role in bone marrow-derived 
      rat mesenchymal stem cells (MSCs), which differentiate into cardiomyocytes and/or 
      cells with comparable phenotypes under low oxygen conditions. Cx43 and 
      Cx43-related factor expression profiles were compared between normoxic and 
      hypoxic conditions according to exposure time, and Sgsm3 gene knockdown (KD) 
      using siRNA transfection was performed to validate the interaction between SGSM3 
      and Cx43 and to determine the roles of SGSM3 in rat MSCs. We identified that 
      SGSM3 interacts with Cx43 in MSCs under different oxygen conditions and that 
      Sgsm3 knockdown inhibits apoptosis and cardiomyocyte differentiation under 
      hypoxic stress. SGSM3/Sgsm3 probably has an effect on MSC survival and thus 
      therapeutic potential in diseased hearts, but SGSM3 may worsen the development of 
      MSC-based therapeutic approaches in regenerative medicine. This study was 
      performed to help us better understand the mechanisms involved in the therapeutic 
      efficacy of MSCs, as well as provide data that could be used pharmacologically.
FAU - Jung, Seung Eun
AU  - Jung SE
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
FAU - Choi, Jung-Won
AU  - Choi JW
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
FAU - Moon, Hanbyeol
AU  - Moon H
AD  - Department of Integrated Omics for Biomedical Sciences, Graduate School, Yonsei 
      University, Seoul, Republic of Korea.
FAU - Oh, Sena
AU  - Oh S
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
FAU - Lim, Soyeon
AU  - Lim S
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
AD  - International St. Mary's Hospital, Catholic Kwandong University, Incheon 
      Metropolitan City, Republic of Korea.
FAU - Lee, Seahyoung
AU  - Lee S
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
AD  - International St. Mary's Hospital, Catholic Kwandong University, Incheon 
      Metropolitan City, Republic of Korea.
FAU - Kim, Sang Woo
AU  - Kim SW
AUID- ORCID: 0000-0002-3144-4822
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
AD  - International St. Mary's Hospital, Catholic Kwandong University, Incheon 
      Metropolitan City, Republic of Korea.
FAU - Hwang, Ki-Chul
AU  - Hwang KC
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Republic of Korea.
AD  - International St. Mary's Hospital, Catholic Kwandong University, Incheon 
      Metropolitan City, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200409
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biomarkers)
RN  - 0 (Connexin 43)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
RN  - EC 3.6.5.2 (Sgsm3 protein, rat)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cell Hypoxia/drug effects
MH  - Connexin 43/metabolism
MH  - Gap Junctions/drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - *Gene Knockdown Techniques
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Mesenchymal Stem Cells/*cytology/drug effects/*metabolism
MH  - Monomeric GTP-Binding Proteins/*metabolism
MH  - Myocytes, Cardiac/drug effects/*metabolism
MH  - Oxygen/pharmacology
MH  - Rats, Sprague-Dawley
MH  - Stress, Physiological/drug effects
MH  - Time Factors
MH  - Wnt Signaling Pathway/drug effects
PMC - PMC7145021
COIS- The authors have declared that no competing interests exist.
EDAT- 2020/04/10 06:00
MHDA- 2020/07/14 06:00
PMCR- 2020/04/09
CRDT- 2020/04/10 06:00
PHST- 2020/02/04 00:00 [received]
PHST- 2020/03/19 00:00 [accepted]
PHST- 2020/04/10 06:00 [entrez]
PHST- 2020/04/10 06:00 [pubmed]
PHST- 2020/07/14 06:00 [medline]
PHST- 2020/04/09 00:00 [pmc-release]
AID - PONE-D-20-02560 [pii]
AID - 10.1371/journal.pone.0231272 [doi]
PST - epublish
SO  - PLoS One. 2020 Apr 9;15(4):e0231272. doi: 10.1371/journal.pone.0231272. 
      eCollection 2020.