PMID- 32272974
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 11
IP  - 1
DP  - 2020 Apr 9
TI  - A comparison of the use of adipose-derived and bone marrow-derived stem cells for 
      peripheral nerve regeneration in vitro and in vivo.
PG  - 153
LID - 10.1186/s13287-020-01661-3 [doi]
LID - 153
AB  - BACKGROUND: To date, it has repeatedly been demonstrated that infusing bone 
      marrow-derived stem cells (BMSCs) into acellular nerve scaffolds can promote and 
      support axon regeneration through a peripheral nerve defect. However, harvesting 
      BMSCs is an invasive and painful process fraught with a low cellular yield. 
      METHODS: In pursuit of alternative stem cell sources, we isolated stem cells from 
      the inguinal subcutaneous adipose tissue of adult Sprague-Dawley rats 
      (adipose-derived stem cells, ADSCs). We used a co-culture system that allows 
      isolated adult mesenchymal stem cells (MSCs) and Schwann cells (SCs) to grow in 
      the same culture medium but without direct cellular contact. We verified SC 
      phenotype in vitro by cell marker analysis and used red fluorescent 
      protein-tagged ADSCs to detect their fate after being injected into a chemically 
      extracted acellular nerve allograft (CEANA). To compare the regenerative effects 
      of CEANA containing either BMSCs or ADSCs with an autograft and CEANA only on the 
      sciatic nerve defect in vivo, we performed histological and functional 
      assessments up to 16 weeks after grafting. RESULTS: In vitro, we observed 
      reciprocal beneficial effects of ADSCs and SCs in the ADSC-SC co-culture system. 
      Moreover, ADSCs were able to survive in CEANA for 5 days after in vitro 
      implantation. Sixteen weeks after grafting, all results consistently showed that 
      CEANA infused with BMSCs or ADSCs enhanced injured sciatic nerve repair compared 
      to the acellular CEANA-only treatment. Furthermore, their beneficial effects on 
      sciatic injury regeneration were comparable as histological and functional 
      parameters evaluated showed no statistically significant differences. However, 
      the autograft group was roundly superior to both the BMSC- or ADSC-loaded CEANA 
      groups. CONCLUSION: The results of the present study show that ADSCs are a viable 
      alternative stem cell source for treating sciatic nerve injury in lieu of BMSCs.
FAU - Zhou, Li Na
AU  - Zhou LN
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China. 
      zhoulina@gzucm.edu.cn.
FAU - Wang, Jia Chuan
AU  - Wang JC
AD  - Department of Pathology, Shenzhen Traditional Chinese Medicine Hospital, The 
      Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, 
      Shenzhen, China.
FAU - Zilundu, Prince Last Mudenda
AU  - Zilundu PLM
AD  - Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University, 
      Guangzhou, China.
FAU - Wang, Ya Qiong
AU  - Wang YQ
AD  - Department of Electron Microscope, Zhongshan School of Medicine, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Guo, Wen Ping
AU  - Guo WP
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China.
FAU - Zhang, Sai Xia
AU  - Zhang SX
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China.
FAU - Luo, Hui
AU  - Luo H
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China.
FAU - Zhou, Jian Hong
AU  - Zhou JH
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China.
FAU - Deng, Ru Dong
AU  - Deng RD
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China.
FAU - Chen, Dong Feng
AU  - Chen DF
AD  - Department of Anatomy, School of basic medical sciences, Guangzhou University of 
      Chinese Medicine, 232 Waihuan East Road, Guangzhou, 510006, Guangdong, China. 
      cdf27212@21cn.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200409
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
SB  - IM
MH  - Adipose Tissue
MH  - Animals
MH  - *Axons
MH  - Bone Marrow
MH  - Cells, Cultured
MH  - *Nerve Regeneration
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve
MH  - Stem Cells
PMC - PMC7147018
OTO - NOTNLM
OT  - Adipose-derived stem cells
OT  - Bone marrow-derived stem cells
OT  - Chemically extracted acellular nerve allograft
OT  - Co-culture
OT  - Peripheral nerve repair
OT  - Schwann cells
COIS- The authors declare that they have no competing interests.
EDAT- 2020/04/11 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/04/09
CRDT- 2020/04/11 06:00
PHST- 2019/11/26 00:00 [received]
PHST- 2020/03/23 00:00 [accepted]
PHST- 2020/01/15 00:00 [revised]
PHST- 2020/04/11 06:00 [entrez]
PHST- 2020/04/11 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/04/09 00:00 [pmc-release]
AID - 10.1186/s13287-020-01661-3 [pii]
AID - 1661 [pii]
AID - 10.1186/s13287-020-01661-3 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2020 Apr 9;11(1):153. doi: 10.1186/s13287-020-01661-3.