PMID- 32275211
OWN - NLM
STAT- MEDLINE
DCOM- 20210127
LR  - 20210127
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 158
DP  - 2020 Jun 15
TI  - A smartphone-based dual detection mode device integrated with two lateral flow 
      immunoassays for multiplex mycotoxins in cereals.
PG  - 112178
LID - S0956-5663(20)30176-7 [pii]
LID - 10.1016/j.bios.2020.112178 [doi]
AB  - In this study, a smartphone-based quantitative dual detection mode device, 
      integrated with gold nanoparticles (GNPs) and time-resolved fluorescence 
      microspheres (TRFMs) lateral flow immunoassays (LFIA) for multiplex mycotoxins in 
      cereals were established. The most frequently used visible light and fluorescence 
      detection modes were integrated in one device. A user-friendly application was 
      self-written to rapidly quantify results. GNPs-LFIA and TRFMs-LFIA were used to 
      detect aflatoxin B1 (AFB1), zearalenone (ZEN), deoxynivalenol (DON), T-2 toxin 
      (T-2), and fumonisin B1 (FB1). The visible limits of detection (vLODs) were 
      10/2.5/1.0/10/0.5, 2.5/0.5/0.5/2.5/0.5 mug/kg for the two methods, respectively. 
      The quantitative limits of detection (qLODs) were 0.59/0.24/0.32/0.9/0.27, 
      0.42/0.10/0.05/0.75/0.04 mug/kg, respectively. The recoveries of both LFIAs ranged 
      from 84.0%-110.0%. A parallel analysis in 30 naturally contaminated cereal 
      samples was conducted by liquid chromatography-tandem mass spectrometry 
      (LC-MS/MS), the results showed good consistency, indicating the practical 
      reliability of the established methods. The developed two smartphone-based LFIAs 
      provide a promising technique for multiplex, highly sensitive, and on-site 
      detection of mycotoxins.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Liu, Zhiwei
AU  - Liu Z
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou, 510642, China.
FAU - Hua, Qicheng
AU  - Hua Q
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou, 510642, China.
FAU - Wang, Jin
AU  - Wang J
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou, 510642, China.
FAU - Liang, Zaoqing
AU  - Liang Z
AD  - College of Mathematics and Infromatics, College of Software Engineering, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Li, Jiahao
AU  - Li J
AD  - College of Mathematics and Infromatics, College of Software Engineering, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Wu, Jinxiao
AU  - Wu J
AD  - Shanxi Institute of Feed and Veterinary Drug control, Taiyuan, 030000, China.
FAU - Shen, Xing
AU  - Shen X
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou, 510642, China.
FAU - Lei, Hongtao
AU  - Lei H
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou, 510642, China. 
      Electronic address: hongtao@scau.edu.cn.
FAU - Li, Xiangmei
AU  - Li X
AD  - Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food 
      Science, South China Agricultural University, Guangzhou, 510642, China. 
      Electronic address: lixiangmei12@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200331
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Mycotoxins)
SB  - IM
MH  - Chromatography, Liquid
MH  - *Edible Grain/chemistry
MH  - Equipment Design
MH  - Food Analysis/instrumentation/*methods
MH  - Food Contamination/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Immunoassay/instrumentation/*methods
MH  - Limit of Detection
MH  - *Mycotoxins/analysis
MH  - Reproducibility of Results
MH  - *Smartphone
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Gold nanoparticles
OT  - Lateral flow immunoassay
OT  - Mycotoxins
OT  - Smartphone
OT  - Time-resolved fluorescence microspheres
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/04/11 06:00
MHDA- 2021/01/28 06:00
CRDT- 2020/04/11 06:00
PHST- 2020/02/20 00:00 [received]
PHST- 2020/03/25 00:00 [revised]
PHST- 2020/03/26 00:00 [accepted]
PHST- 2020/04/11 06:00 [pubmed]
PHST- 2021/01/28 06:00 [medline]
PHST- 2020/04/11 06:00 [entrez]
AID - S0956-5663(20)30176-7 [pii]
AID - 10.1016/j.bios.2020.112178 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2020 Jun 15;158:112178. doi: 10.1016/j.bios.2020.112178. 
      Epub 2020 Mar 31.