PMID- 32275973
OWN - NLM
STAT- MEDLINE
DCOM- 20200803
LR  - 20210602
IS  - 1532-8600 (Electronic)
IS  - 0026-0495 (Print)
IS  - 0026-0495 (Linking)
VI  - 107
DP  - 2020 Jun
TI  - Sustained mitochondrial biogenesis is essential to maintain caloric 
      restriction-induced beige adipocytes.
PG  - 154225
LID - S0026-0495(20)30089-5 [pii]
LID - 10.1016/j.metabol.2020.154225 [doi]
AB  - BACKGROUND: Caloric restriction (CR) delays the onset of metabolic and 
      age-related disorders. Recent studies have demonstrated that formation of beige 
      adipocytes induced by CR is strongly associated with extracellular remodeling in 
      adipose tissue, decrease in adipose tissue inflammation, and improved systemic 
      metabolic homeostasis. However, beige adipocytes rapidly transition to white upon 
      CR withdrawal through unclear mechanisms. MATERIALS AND METHODS: Six-week old 
      C57BL6 mice were fed with 40% CR chow diet for 6 weeks. Subsequently, one group 
      of mice was switched back to ad libitum chow diet, which was continued for 
      additional 2 weeks. Adipose tissues were assessed histologically and 
      biochemically for beige adipocytes. RESULTS: Beige adipocytes induced by CR 
      rapidly transition to white adipocytes when CR is withdrawn independent of 
      parkin-mediated mitophagy. We demonstrate that the involution of mitochondria 
      during CR withdrawal is strongly linked with a decrease in mitochondrial 
      biogenesis. We further demonstrate that beige-to-white fat transition upon beta3-AR 
      agonist-withdrawal could be attenuated by CR, partly via maintenance of 
      mitochondrial biogenesis. CONCLUSION: In the model of CR, our study highlights 
      the dominant role of mitochondrial biogenesis in the maintenance of beige 
      adipocytes. We propose that loss of beige adipocytes upon beta3-AR agonist 
      withdrawal could be attenuated by CR.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Mooli, Raja Gopal Reddy
AU  - Mooli RGR
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Mukhi, Dhanunjay
AU  - Mukhi D
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Watt, Mikayla
AU  - Watt M
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Edmunds, Lia
AU  - Edmunds L
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Xie, Bingxian
AU  - Xie B
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Capooci, Joseph
AU  - Capooci J
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Reslink, Matthew
AU  - Reslink M
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Eze, Chetachukwu
AU  - Eze C
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Mills, Amanda
AU  - Mills A
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Stolz, Donna B
AU  - Stolz DB
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Jurczak, Michael
AU  - Jurczak M
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America.
FAU - Ramakrishnan, Sadeesh K
AU  - Ramakrishnan SK
AD  - Division of Endocrinology and Metabolism, Department of Medicine, University of 
      Pittsburgh, Pittsburgh, PA 15262., United States of America. Electronic address: 
      ramaks@pitt.edu.
LA  - eng
GR  - K99 DK110537/DK/NIDDK NIH HHS/United States
GR  - R00 DK110537/DK/NIDDK NIH HHS/United States
GR  - P30 DK120531/DK/NIDDK NIH HHS/United States
GR  - S10 RR019003/RR/NCRR NIH HHS/United States
GR  - R01 DK119627/DK/NIDDK NIH HHS/United States
GR  - R01 DK114012/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20200407
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (Adrenergic beta-3 Receptor Agonists)
RN  - 0 (Insulin)
SB  - IM
MH  - Adipocytes, Beige/*physiology
MH  - Adipocytes, White/physiology
MH  - Adipose Tissue/cytology
MH  - Adrenergic beta-3 Receptor Agonists/pharmacology
MH  - Animals
MH  - Body Composition
MH  - *Caloric Restriction
MH  - Cell Fusion
MH  - Diet
MH  - Insulin/blood
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitophagy
MH  - *Organelle Biogenesis
MH  - Oxygen Consumption
MH  - Primary Cell Culture
PMC - PMC7284285
MID - NIHMS1582607
OTO - NOTNLM
OT  - Beige adipocytes
OT  - Caloric restriction
OT  - Fission
OT  - Fusion
OT  - Mitochondrial biogenesis
OT  - Mitochondrial dynamics
OT  - Mitophagy
COIS- Declaration of competing interest The authors declare no conflict of interests.
EDAT- 2020/04/11 06:00
MHDA- 2020/08/04 06:00
PMCR- 2021/06/01
CRDT- 2020/04/11 06:00
PHST- 2020/01/29 00:00 [received]
PHST- 2020/03/27 00:00 [revised]
PHST- 2020/04/05 00:00 [accepted]
PHST- 2020/04/11 06:00 [pubmed]
PHST- 2020/08/04 06:00 [medline]
PHST- 2020/04/11 06:00 [entrez]
PHST- 2021/06/01 00:00 [pmc-release]
AID - S0026-0495(20)30089-5 [pii]
AID - 10.1016/j.metabol.2020.154225 [doi]
PST - ppublish
SO  - Metabolism. 2020 Jun;107:154225. doi: 10.1016/j.metabol.2020.154225. Epub 2020 
      Apr 7.