PMID- 32277655
OWN - NLM
STAT- MEDLINE
DCOM- 20201209
LR  - 20201214
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 1
DP  - 2020 Jan-Feb
TI  - Novel insights into laryngeal squamous cell carcinoma from association study of 
      aberrantly expressed miRNAs, lncRNAs and clinical features in Bulgarian patients.
PG  - 357-366
AB  - PURPOSE: Laryngeal cancer is one of most common and aggressive head and neck 
      cancers with poor prognosis and great necessity for improvement of treatment 
      modalities. MicroRNAs (miRs) are among the most investigated molecules recently 
      due to their potential as diagnostic and prognostic biomarkers in cancer. The 
      purpose of our study was to explore the association of certain 
      clinicopathological features with the expression levels of some known cancer 
      associated non-coding (nc) RNAs: miR-21 and miR-31 in both of their isoforms, 
      miR-145-5p, miR-55-5p, miR-196a-5p, miR-210-3p, miR-221-3p, miR-222-3p, 
      miR-424-5p, lncRNA MALAT1 and lncRNA HOTAIR. METHODS: Expression levels of the 
      chosen markers were investigated in laryngeal squamous cell carcinoma (LSCC) and 
      normal samples in 82 Bulgarian patients via RT-qPCR, and the results were 
      analyzed with SPSS v23.0 statistical software. RESULTS: All of the explored 
      ncRNAs were significantly deregulated in LSCC samples, suggesting their 
      involvement in laryngeal carcinogenesis. New significant association were found 
      between the expression levels of miR-21-5p, miR-222-3p, HOTAIR and family 
      history. Moreover, miR-424-5p showed potential as marker for subglottic LSCC 
      location, and "passenger" miR-31-3p was significantly upregulated in well and 
      moderately differentiated LSCC. CONCLUSION: Our results enrich the knowledge 
      about ncRNA involvement in LSCC tumorigenesis. Further studies are needed to 
      evaluate the clinical utility of the differently expressed ncRNAs as potential 
      diagnostic and prognostic biomarkers in LSCC.
FAU - Garo Kyurkchiyan, Silva
AU  - Garo Kyurkchiyan S
AD  - Molecular Medicine Center, Department of Medical Chemistry and Biochemistry, 
      Medical University, Sofia, Bulgaria.
FAU - Miroslavov Popov, Todor
AU  - Miroslavov Popov T
FAU - Stancheva, Gergana
AU  - Stancheva G
FAU - Rangachev, Julian
AU  - Rangachev J
FAU - Ivanov Mitev, Vanyo
AU  - Ivanov Mitev V
FAU - Petrova Popova, Diana
AU  - Petrova Popova D
FAU - Petrova Kaneva, Radka
AU  - Petrova Kaneva R
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bulgaria
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - RNA, Long Noncoding/*metabolism
EDAT- 2020/04/12 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/04/12 06:00
PHST- 2020/04/12 06:00 [entrez]
PHST- 2020/04/12 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Jan-Feb;25(1):357-366.