PMID- 32277664
OWN - NLM
STAT- MEDLINE
DCOM- 20201209
LR  - 20210908
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 1
DP  - 2020 Jan-Feb
TI  - The anticancer effects of 7-Methoxyheptaphylline against the human retinoblastoma 
      cells are facilitated via S-phase cell cycle arrest, mitochondrial apoptosis and 
      inhibition of Wnt/beta-catenin signalling pathway.
PG  - 421-426
AB  - PURPOSE: The main purpose of the current study was to evaluate the anticancer 
      action of 7-Methoxyheptaphylline in Y-79 human retinoblastoma cells along with 
      evaluating its effects on cellular apoptosis, cell cycle phase distribution and 
      Wnt/beta-catenin signalling pathway. METHODS: The retinoblastoma cell line RbY-79 
      was used in this study. Cell viability was assessed by WTS-1 assay while 
      apoptotic studies were carried out by DAPI, acridine orange (AO)/ethidium bromide 
      (EB), annexin V + propidium iodide (PI) staining using fluorescence microscopy 
      and flow cytometry. Effects on cell cycle progression were studied using Annexin 
      V/PI staining in combination with flow cytometry. Western blot assay was used to 
      examine the effects on Bax, Bcl-2 and proteins associated with Wnt/beta-catenin 
      signalling pathway. RESULTS: The results indicated that 7-Methoxyheptaphylline 
      suppressed the viability of the Y-79 cells concentration-dependently with IC50 
      value of 15.5 muM. The percentage of the DAPI-positive cells showed a significant 
      upsurge reminiscent of the apoptosis in the Y-79 retinoblastoma cells. 
      7-Methoxyheptaphylline also caused considerable nuclear fragmentation of the Y-79 
      retinoblastoma cells, representative of apoptosis. The apoptosis percentage 
      increased significantly as the dose of the 7-Methoxyheptaphylline increased from 
      0 to 12, 24 and 48 microM. The molecule caused upregulation of Bax and downregulation 
      of Bcl-2 in Y-79 retinoblastoma cells. 7-Methoxyheptaphylline also caused S-phase 
      cell cycle arrest with concomitant concentration-dependent decline in the 
      expression levels of cyclin A, E and D1. It was also seen that increasing doses 
      of 7-Methoxyheptaphylline led to a dose-dependent decline in the expression 
      levels of wnt-13a and beta-catenin. CONCLUSIONS: In conclusion, it is believed that 
      the molecule may prove to be a promising anticancer agent for the treatment of 
      retinoblastoma.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, Hubei, 
      China, 430060.
FAU - He, Tao
AU  - He T
FAU - Wu, Li
AU  - Wu L
FAU - Cao, Ting
AU  - Cao T
FAU - Zheng, Hongmei
AU  - Zheng H
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (7-methoxyheptaphylline)
RN  - 0 (Carbazoles)
SB  - IM
ECI - J BUON. 2021 May-Jun;26(3):1182. PMID: 34268999
MH  - Apoptosis/*drug effects
MH  - Carbazoles/pharmacology/*therapeutic use
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Humans
MH  - Mitochondria/*drug effects
MH  - Retinoblastoma/*drug therapy/pathology
MH  - S Phase/*drug effects
MH  - Wnt Signaling Pathway/*drug effects
EDAT- 2020/04/12 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/04/12 06:00
PHST- 2020/04/12 06:00 [entrez]
PHST- 2020/04/12 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Jan-Feb;25(1):421-426.