PMID- 32277671
OWN - NLM
STAT- MEDLINE
DCOM- 20201209
LR  - 20201214
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 1
DP  - 2020 Jan-Feb
TI  - MicroRNA-187 suppresses the proliferation migration and invasion of human 
      osteosarcoma cells by targeting MAPK7.
PG  - 472-478
AB  - PURPOSE: Osteosarcoma is one of the rare but fatal malignancies. The high 
      metastatic rate, late diagnosis, emergence of drug resistance against drugs such 
      as doxorubicin, and the lack of therapeutic targets obstructs the treatment of 
      osteosarcoma. This study was undertaken to investigate the role and therapeutic 
      potential of miR-187 in human osteosarcoma cells. METHODS: The WST-1 
      proliferation assay was used for investigation of cell viability. Transfections 
      were carried out by Lipofectamine 2000 reagent. The qRT-PCR was used for 
      expression analysis. DAPI, acridine orange (AO)/ethidium bromide (EB) and Annexin 
      V/propidium iodide (PI) assay were used for apoptosis. Western blot analysis was 
      used for the determination of protein expression. RESULTS: The expression of 
      miR-187 was significantly downregulated in human osteosarcoma cells. Out of all 
      osteosarcoma cell lines the SAOS-2 showed the lowest expression of miR-187 and 
      therefore this cell line was selected for further studies. Overexpression of 
      miR-187 caused significant inhibition in the proliferation of SAOS-2 osteosarcoma 
      cells. The miR-187-triggered growth inhibition was found to be mainly due to 
      induction of G2/M phase cell cycle arrest of the SAOS-2 cells. The G2/M cell 
      cycle arrest was also accompanied by depletion of Cyclin-B1 expression. 
      Additionally, miR-187 enhanced the chemosensitivity of the osteosarcoma cells to 
      doxorubicin. The wound healing and transwell assay showed that miR-187 
      overexpression resulted in the suppression of migration and invasion of the 
      SAOS-2 osteosarcoma cells. In silico analysis showed that miR-187 exerts its 
      effects by inhibiting mitogen activated protein kinase 7 (MAPK7). The expression 
      of MAPK7 was found to be significantly upregulated in osteosarcoma cells and 
      overexpression of MAPK7 could nullify the effects of miR-187 on the proliferation 
      of the osteosarcoma cells.
FAU - Liu, Min
AU  - Liu M
AD  - Department of Orthopaedics, the Second Affiliated Hospital of Soochow University, 
      Suzhou, Jiangsu 215101, China.
FAU - Wu, Lifen
AU  - Wu L
FAU - Cai, Chunyuan
AU  - Cai C
FAU - Liu, Liangle
AU  - Liu L
FAU - Xu, Youjia
AU  - Xu Y
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (MIRN187 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 80168379AG (Doxorubicin)
RN  - EC 2.7.11.24 (MAPK7 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 7)
SB  - IM
MH  - Antibiotics, Antineoplastic/pharmacology
MH  - Bone Neoplasms/drug therapy/enzymology/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - Down-Regulation
MH  - Doxorubicin/pharmacology
MH  - G2 Phase Cell Cycle Checkpoints/physiology
MH  - Humans
MH  - M Phase Cell Cycle Checkpoints/physiology
MH  - MicroRNAs/biosynthesis/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 7/*metabolism
MH  - Neoplasm Invasiveness
MH  - Osteosarcoma/drug therapy/enzymology/*genetics/pathology
EDAT- 2020/04/12 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/04/12 06:00
PHST- 2020/04/12 06:00 [entrez]
PHST- 2020/04/12 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Jan-Feb;25(1):472-478.