PMID- 32278689
OWN - NLM
STAT- MEDLINE
DCOM- 20201231
LR  - 20201231
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 392
IP  - 2
DP  - 2020 Jul 15
TI  - Twist1 promotes dendritic cell-mediated antitumor immunity.
PG  - 112003
LID - S0014-4827(20)30225-1 [pii]
LID - 10.1016/j.yexcr.2020.112003 [doi]
AB  - Dendritic cells (DCs) play a central role in autoimmunity, immune homeostasis, 
      and presentation of tumor antigens to T cells in order to prime antitumor 
      responses. The number of tumor-infiltrating DCs is associated with survival and 
      prognosis in cancer. Twist1 is a well-known regulator of tumor initiation and 
      promotion, but whether and how DC-derived Twist1 regulates antitumor responses 
      remains poorly understood. Here, we generated a mouse line with Twist1 
      conditionally depleted in DCs and found that Twist1-deficiency in DCs did not 
      affect the DCs and T cell homeostasis under steady-state conditions; however, in 
      melanoma models, the proportion of conventional DCs (cDCs) in draining lymph 
      nodes (DLNs) was significantly decreased. Accordingly, a decreased ratio and 
      number of tumor-infiltrating cDCs were observed, which reduced the recruitment of 
      tumor-infiltrating T cells. Furthermore, production of IFN-gamma, a crucial antitumor 
      factor, by T cells, was dramatically decreased, which can further dampen the T 
      cell antitumor functions. Collectively, our data indicate that Twist1 in DCs 
      regulates antitumor functions by maintain the number of tumor-infiltrating DCs 
      and T cells, and their antitumor activity.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Luo, Yuechen
AU  - Luo Y
AD  - State Key Laboratory of Experimental Hematology, National Clinical Research 
      Center for Blood Disease, Institute of Hematology & Hospital of Blood Disease, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 
      300020, China.
FAU - Chen, Jiadi
AU  - Chen J
AD  - Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, 
      Fujian Medical University Union Hospital, Fuzhou, China.
FAU - Liu, Maolan
AU  - Liu M
AD  - Department of Hematology, The First Affiliated Hospital of Kunming Medical 
      University, Hematology Research Center of Yunnan Province, Kunming, 650032, 
      China.
FAU - Chen, Song
AU  - Chen S
AD  - State Key Laboratory of Experimental Hematology, National Clinical Research 
      Center for Blood Disease, Institute of Hematology & Hospital of Blood Disease, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 
      300020, China.
FAU - Su, Xiuhua
AU  - Su X
AD  - State Key Laboratory of Experimental Hematology, National Clinical Research 
      Center for Blood Disease, Institute of Hematology & Hospital of Blood Disease, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 
      300020, China.
FAU - Su, Jing
AU  - Su J
AD  - Department of Hematology, The First Affiliated Hospital of Kunming Medical 
      University, Hematology Research Center of Yunnan Province, Kunming, 650032, 
      China.
FAU - Zhao, Chunxiao
AU  - Zhao C
AD  - State Key Laboratory of Experimental Hematology, National Clinical Research 
      Center for Blood Disease, Institute of Hematology & Hospital of Blood Disease, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 
      300020, China.
FAU - Han, Zhongchao
AU  - Han Z
AD  - State Key Laboratory of Experimental Hematology, National Clinical Research 
      Center for Blood Disease, Institute of Hematology & Hospital of Blood Disease, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 
      300020, China.
FAU - Shi, Mingxia
AU  - Shi M
AD  - Department of Hematology, The First Affiliated Hospital of Kunming Medical 
      University, Hematology Research Center of Yunnan Province, Kunming, 650032, 
      China. Electronic address: shmxia2002@sina.com.
FAU - Ma, Xiaotong
AU  - Ma X
AD  - State Key Laboratory of Experimental Hematology, National Clinical Research 
      Center for Blood Disease, Institute of Hematology & Hospital of Blood Disease, 
      Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 
      300020, China. Electronic address: maxt@ihcams.ac.cn.
FAU - Huang, Huifang
AU  - Huang H
AD  - Central Laboratory, Fujian Medical University Union Hospital, Fuzhou, China. 
      Electronic address: huanghuif@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200410
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Twist-Related Protein 1)
RN  - 136253-27-5 (Twist1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/immunology
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Dendritic Cells/*immunology/metabolism/pathology
MH  - Immunity, Cellular/*immunology
MH  - Lymphocytes, Tumor-Infiltrating/*immunology
MH  - Melanoma, Experimental/*immunology/metabolism/pathology
MH  - Mice
MH  - Mice, Knockout
MH  - Twist-Related Protein 1/*physiology
OTO - NOTNLM
OT  - Antitumor
OT  - Dendritic cells
OT  - IFN-gamma
OT  - Twist1
COIS- Declaration of competing interest All authors have declared no conflicts of 
      interest.
EDAT- 2020/04/13 06:00
MHDA- 2021/01/01 06:00
CRDT- 2020/04/13 06:00
PHST- 2019/11/25 00:00 [received]
PHST- 2020/04/03 00:00 [revised]
PHST- 2020/04/05 00:00 [accepted]
PHST- 2020/04/13 06:00 [pubmed]
PHST- 2021/01/01 06:00 [medline]
PHST- 2020/04/13 06:00 [entrez]
AID - S0014-4827(20)30225-1 [pii]
AID - 10.1016/j.yexcr.2020.112003 [doi]
PST - ppublish
SO  - Exp Cell Res. 2020 Jul 15;392(2):112003. doi: 10.1016/j.yexcr.2020.112003. Epub 
      2020 Apr 10.