PMID- 32279463
OWN - NLM
STAT- MEDLINE
DCOM- 20200921
LR  - 20200921
IS  - 2523-3548 (Electronic)
IS  - 2523-3548 (Linking)
VI  - 40
IP  - 4
DP  - 2020 Apr
TI  - Association of chromosome 7 aneuploidy measured by fluorescence in situ 
      hybridization assay with muscular invasion in bladder cancer.
PG  - 167-180
LID - 10.1002/cac2.12017 [doi]
AB  - BACKGROUND: The preoperative prediction of muscular invasion status is important 
      for adequately treating bladder cancer (BC) but nevertheless, there are some 
      existing dilemmas in the current preoperative diagnostic accuracy of BC with 
      muscular invasion. Here, we investigated the potential association between the 
      fluorescence in situ hybridization (FISH) assay and muscular invasion among 
      patients with BC. A cytogenetic-clinical nomogram for the individualized 
      preoperative differentiation of muscle-invasive BC (MIBC) from 
      non-muscle-invasive BC (NMIBC) is also proposed. METHODS: All eligible BC 
      patients were preoperatively tested using a FISH assay, which included 4 sites 
      (chromosome-specific centromeric probe [CSP] 3, 7, and 17, and gene 
      locus-specific probe [GLP]-p16 locus). The correlation between the FISH assay and 
      BC muscular invasion was evaluated using the Chi-square tests. In the training 
      set, univariate and multivariate logistic regression analyses were used to 
      develop a cytogenetic-clinical nomogram for preoperative muscular invasion 
      prediction. Then, we assessed the performance of the nomogram in the training set 
      with respect to its discriminatory accuracy and calibration for predicting 
      muscular invasion, and clinical usefulness, which were then validated in the 
      validation set. Moreover, model comparison was set to evaluate the discrimination 
      and clinical usefulness between the nomogram and the individual variables 
      incorporated in the nomogram. RESULTS: Muscular invasion was more prevalent in BC 
      patients with positive CSP3, CSP7 and CSP17 status (OR [95% CI], 2.724 [1.555 to 
      4.774], P < 0.001; 3.406 [1.912 to 6.068], P < 0.001 and 2.483 [1.436 to 4.292], 
      P = 0.001, respectively). Radiology-determined tumor size, radiology-determined 
      clinical tumor stage and CSP7 status were identified as independent risk factors 
      of BC muscular invasion by the multivariate regression analysis in the training 
      set. Then, a cytogenetic-clinical nomogram incorporating these three independent 
      risk factors was constructed and was observed to have satisfactory discrimination 
      in the training (AUC 0.784; 95% CI: 0.715 to 0.853) and validation (AUC 0.743; 
      95% CI: 0.635 to 0.850) set. The decision curve analysis (DCA) indicated the 
      clinical usefulness of our nomogram. In models comparison, using the receiver 
      operator characteristic (ROC) analyses, the nomogram showed higher discriminatory 
      accuracy than any variables incorporated in the nomogram alone and the DCAs also 
      identified the nomogram as possessing the highest net benefits at wide range of 
      threshold probabilities. CONCLUSION: CSP7 status was identified as an independent 
      factor for predicting muscular invasion in BC patients and was successfully 
      incorporated in a clinical nomogram combining the results of the FISH assay with 
      clinical risk factors.
CI  - (c) 2020 The Authors. Cancer Communications published by John Wiley & Sons 
      Australia, Ltd. on behalf of Sun Yat-sen University Cancer Center.
FAU - Diao, Xiayao
AU  - Diao X
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Cai, Jinhua
AU  - Cai J
AD  - Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
FAU - Zheng, Junjiong
AU  - Zheng J
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Kong, Jianqiu
AU  - Kong J
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Wu, Shaoxu
AU  - Wu S
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Yu, Hao
AU  - Yu H
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Huang, Hao
AU  - Huang H
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Xie, Weibin
AU  - Xie W
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Chen, Xiong
AU  - Chen X
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Huang, Chengran
AU  - Huang C
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
FAU - Huang, Lifang
AU  - Huang L
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
AD  - State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510120, P. 
      R. China.
FAU - Qin, Haide
AU  - Qin H
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
AD  - State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510120, P. 
      R. China.
FAU - Huang, Jian
AU  - Huang J
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
AD  - State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510120, P. 
      R. China.
FAU - Lin, Tianxin
AU  - Lin T
AUID- ORCID: 0000-0003-3180-8697
AD  - Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong, 510120, P. R. China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      Guangdong, 510120, P. R. China.
AD  - State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, 510120, P. 
      R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200412
PL  - United States
TA  - Cancer Commun (Lond)
JT  - Cancer communications (London, England)
JID - 101723675
SB  - IM
MH  - Aged
MH  - Aneuploidy
MH  - Chromosomes, Human, Pair 7/*metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Urinary Bladder Neoplasms/*genetics/pathology
PMC - PMC7170658
OTO - NOTNLM
OT  - chromosome aneuploidy
OT  - fluorescence in situ hybridization
OT  - muscular invasion
OT  - nomogram
OT  - preoperative prediction
OT  - urinary bladder neoplasms
COIS- The authors declare that they have no competing interests.
EDAT- 2020/04/13 06:00
MHDA- 2020/09/22 06:00
PMCR- 2020/04/12
CRDT- 2020/04/13 06:00
PHST- 2019/12/22 00:00 [received]
PHST- 2020/02/27 00:00 [revised]
PHST- 2020/03/02 00:00 [accepted]
PHST- 2020/04/13 06:00 [pubmed]
PHST- 2020/09/22 06:00 [medline]
PHST- 2020/04/13 06:00 [entrez]
PHST- 2020/04/12 00:00 [pmc-release]
AID - CAC212017 [pii]
AID - 10.1002/cac2.12017 [doi]
PST - ppublish
SO  - Cancer Commun (Lond). 2020 Apr;40(4):167-180. doi: 10.1002/cac2.12017. Epub 2020 
      Apr 12.