PMID- 32281654
OWN - NLM
STAT- MEDLINE
DCOM- 20210218
LR  - 20210218
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 91
IP  - 12
DP  - 2020 Dec
TI  - Inhibition of GDF11 could promote bone healing in the tooth extraction socket and 
      facilitate mesenchymal stem cell osteogenic differentiation in T2DM pigs.
PG  - 1645-1652
LID - 10.1002/JPER.20-0011 [doi]
AB  - BACKGROUND: Growth differentiation factor 11 (GDF11) might be a key factor 
      responsible for the weakening of mesenchymal stem cell (MSC) osteogenic 
      differentiation in tooth extraction sockets in patients with type 2 diabetes 
      mellitus (T2DM). This study aimed to confirm that inhibition of GDF11 could 
      promote bone healing in tooth extraction sockets and facilitate MSC osteogenic 
      differentiation under T2DM conditions. METHODS: Three streptozotocin-induced T2DM 
      pig models and two control pig models were established. The T2DM pigs were 
      treated with an intrasocket injection of GDF11 inhibitor in the left mandible, 
      whereas the right side was maintained for natural healing. The postextraction 
      socket healing of the T2DM pigs was compared with that of nondiabetic controls. 
      Healing was quantitatively verified by microcomputed tomography, and the GDF11 
      expression level was detected. MSCs from T2DM pig sockets were cultured and 
      treated with a GDF11 inhibitor. The osteogenic differentiation ability of MSCs 
      was also compared among groups. RESULTS: The expression of GDF11 in the tooth 
      extraction sockets from T2DM pigs increased significantly post extraction. Bone 
      healing was promoted by periodic injection of the GDF11 inhibitor into the 
      extraction sockets of T2DM pigs. Furthermore, the osteogenic differentiation 
      ability of T2DM-MSCs was improved in pigs treated with the GDF11 inhibitor. 
      CONCLUSIONS: GDF11 inhibition could promote bone healing in the tooth extraction 
      socket and facilitate MSC osteogenic differentiation under T2DM conditions. GDF11 
      could be a potential therapeutic target for undesirable alveolar bone healing in 
      T2DM patients.
CI  - (c) 2020 American Academy of Periodontology.
FAU - Wang, Shuyan
AU  - Wang S
AD  - Department of Oral Disease, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Implant Dentistry, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
FAU - Shi, Shaojie
AU  - Shi S
AD  - Department of Implant Dentistry, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
FAU - Wang, Xingxing
AU  - Wang X
AD  - Department of Implant Dentistry, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
FAU - He, Chunxia
AU  - He C
AD  - Institute of Basic Translational Medicine, Xi'an Medical University, Xi'an, 
      Shaanxi, China.
FAU - Yuan, Lijuan
AU  - Yuan L
AD  - Department of General Surgery, Tangdu Hospital, Air Force Medical University, 
      Xi'an, China.
FAU - Ding, Feng
AU  - Ding F
AD  - Department of Implant Dentistry, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
FAU - Song, Yingliang
AU  - Song Y
AD  - Department of Implant Dentistry, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
FAU - Zhang, Sijia
AU  - Zhang S
AUID- ORCID: 0000-0002-7549-782X
AD  - Department of Implant Dentistry, School of Stomatology, The Air Force Medical 
      University, Xi'an, China.
AD  - Department of Biochemistry and Molecular Biology, Center for DNA Typing, Air 
      Force Medical University, Xi'an, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200508
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (GDF11 protein, human)
RN  - 0 (Growth Differentiation Factors)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Proteins
MH  - Cell Differentiation
MH  - *Diabetes Mellitus, Type 2
MH  - Growth Differentiation Factors
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Osteogenesis
MH  - Swine
MH  - Tooth Extraction
MH  - Tooth Socket
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - bone regeneration
OT  - growth factors
OT  - mesenchymal stem cells
OT  - tooth socket
OT  - type 2 diabetes mellitus
EDAT- 2020/04/14 06:00
MHDA- 2021/02/20 06:00
CRDT- 2020/04/14 06:00
PHST- 2020/01/09 00:00 [received]
PHST- 2020/02/29 00:00 [revised]
PHST- 2020/03/18 00:00 [accepted]
PHST- 2020/04/14 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/04/14 06:00 [entrez]
AID - 10.1002/JPER.20-0011 [doi]
PST - ppublish
SO  - J Periodontol. 2020 Dec;91(12):1645-1652. doi: 10.1002/JPER.20-0011. Epub 2020 
      May 8.