PMID- 32283616
OWN - NLM
STAT- MEDLINE
DCOM- 20210106
LR  - 20210106
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 7
DP  - 2020 Apr 9
TI  - Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium 
      (Crustacea: Decapoda: Palaemonidae).
LID - 10.3390/ijms21072599 [doi]
LID - 2599
AB  - Freshwater prawns of the genus Macrobrachium are one of the important components 
      of circumtropical marine, estuarine, and freshwater environments. They have been 
      extensively exploited for human consumption for many years. More than 250 species 
      reflect the evolutionary success of this highly diversified group, with a complex 
      and challenging taxonomy due to morphological variations and vast geographical 
      distribution. Although genetic approaches have been used to clarify phylogenetic 
      and taxonomic aspects of Macrobrachium species, cytogenetic information is still 
      very scarce and mostly focused on chromosome number and morphology. Here, we 
      present chromosome data for three species from the Neotropical region, M. 
      carcinus, M. acanthurus, and M. amazonicum, and one species from the Oriental 
      region, M. rosenbergii. Using conventional cytogenetic approaches and chromosome 
      mapping of repetitive DNAs by fluorescence in situ hybridization (FISH), we 
      identified numerical diversification of the diploid set, within and between both 
      zoogeographic regions. These included M. acanthurus and M. amazonicum sharing 
      diploid chromosomes of 98, while M. carcinus has 94, and M. rosenbergii has 118 
      chromosomes. Argentophilic sites are also variable in number, but they occur in a 
      much higher number than 18S rDNA, representing two to 10 sites within the study 
      species. Microsatellites repeat motifs are also abundant in the chromosomes, with 
      a co-localization and uniform distribution along the chromosome arms, but 
      completely absent in the AT-rich centromeric regions. As a whole, our study 
      suggests that the 2n divergence was followed by a considerable rDNA 
      diversification. The abundance of the exceptional amount of microsatellite 
      sequences in the chromosomes also suggests that they are essential components of 
      the Macrobrachium genome and, therefore, maintained as a shared feature by the 
      species, the reason for which is yet unknown.
FAU - Molina, Wagner F
AU  - Molina WF
AUID- ORCID: 0000-0002-6695-0952
AD  - Departamento de Biologia Celular e Genetica, Centro de Biociencias, Universidade 
      Federal do Rio Grande do Norte, Natal, RN 59078970, Brazil.
FAU - Costa, Gideao W W F
AU  - Costa GWWF
AD  - Departamento de Biologia Celular e Genetica, Centro de Biociencias, Universidade 
      Federal do Rio Grande do Norte, Natal, RN 59078970, Brazil.
FAU - Cunha, Inailson M C
AU  - Cunha IMC
AD  - Departamento de Biologia Celular e Genetica, Centro de Biociencias, Universidade 
      Federal do Rio Grande do Norte, Natal, RN 59078970, Brazil.
FAU - Bertollo, Luiz A C
AU  - Bertollo LAC
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, SP C.P. 676, Brazil.
FAU - Ezaz, Tariq
AU  - Ezaz T
AUID- ORCID: 0000-0003-4763-1347
AD  - Institute for Applied Ecology, University of Canberra, Canberra, ACT 2617, 
      Australia.
FAU - Liehr, Thomas
AU  - Liehr T
AUID- ORCID: 0000-0003-1672-3054
AD  - Institute of Human Genetics, University Hospital Jena, 7747 Jena, Germany.
FAU - Cioffi, Marcelo B
AU  - Cioffi MB
AUID- ORCID: 0000-0003-4340-1464
AD  - Laboratorio de Citogenetica de Peixes, Departamento de Genetica e Evolucao, 
      Universidade Federal de Sao Carlos, Sao Carlos, SP C.P. 676, Brazil.
LA  - eng
GR  - 442664/2015-0/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 442626/2019-3/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 301458/2019-7/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 302449/2018-3/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
GR  - 401962/2016-4/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/
PT  - Journal Article
DEP - 20200409
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - *Cytogenetic Analysis
MH  - DNA, Ribosomal/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Microsatellite Repeats
MH  - Palaemonidae/classification/*genetics
PMC - PMC7178241
OTO - NOTNLM
OT  - SSRs
OT  - crustacea cytogenetics
OT  - diploid variation
OT  - karyotype evolution
OT  - rDNA
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/04/15 06:00
MHDA- 2021/01/07 06:00
PMCR- 2020/04/01
CRDT- 2020/04/15 06:00
PHST- 2020/03/21 00:00 [received]
PHST- 2020/04/07 00:00 [revised]
PHST- 2020/04/07 00:00 [accepted]
PHST- 2020/04/15 06:00 [entrez]
PHST- 2020/04/15 06:00 [pubmed]
PHST- 2021/01/07 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - ijms21072599 [pii]
AID - ijms-21-02599 [pii]
AID - 10.3390/ijms21072599 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Apr 9;21(7):2599. doi: 10.3390/ijms21072599.