PMID- 32283836
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 8
IP  - 4
DP  - 2020 Apr 9
TI  - Epigallocatechin-3-Gallate Induces Apoptosis as a TRAIL Sensitizer via Activation 
      of Caspase 8 and Death Receptor 5 in Human Colon Cancer Cells.
LID - 10.3390/biomedicines8040084 [doi]
LID - 84
AB  - Though epigallocatechin-3-gallate (EGCG), a major compound of green tea, has 
      anti-diabetes, anti-obesity, anti-inflammatory, and antitumor effects, the 
      underlying antitumor molecular mechanism of EGCG was not fully understood so far. 
      Here the sensitizing effect of EGCG to tumor-necrosis-factor-related 
      apoptosis-inducing ligand (TRAIL) was examined in colorectal cancers. Cotreatment 
      of EGCG and TRAIL synergistically enhanced cytotoxicity and sub G1 accumulation, 
      increased the number of terminal deoxynucleotidyl transferase-dT-mediated dUTP 
      nick end labelling (TUNEL)-positive cells in SW480 and HCT116 cells. Furthermore, 
      this cotreatment promoted the cleavages of poly (adenosine diphosphate-ribose) 
      polymerase (PARP) and induced caspase 8 activation compared to TRAIL or EGCG 
      alone in SW480 and HCT116 cells. Of note, cotreatment of EGCG and TRAIL increased 
      the expression of death receptor 5 (DR5) at protein and mRNA levels and also DR5 
      cell surface level in colon cancer cells. Conversely, depletion of DR5 reduced 
      the apoptotic activity of cotreatment of EGCG and TRAIL to increase cytotoxicity, 
      sub-G1 population and PARP cleavages in colon cancer cells. Overall, our findings 
      provide evidence that EGCG can be a sensitizer of TRAIL via DR5 and caspase 8 
      mediated apoptosis in colorectal cancer cells.
FAU - Kwon, Oh Sung
AU  - Kwon OS
AD  - College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
FAU - Jung, Ji Hoon
AU  - Jung JH
AD  - College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
FAU - Shin, Eun Ah
AU  - Shin EA
AD  - College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
FAU - Park, Ji Eon
AU  - Park JE
AD  - College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
FAU - Park, Woon Yi
AU  - Park WY
AD  - College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
FAU - Kim, Sung-Hoon
AU  - Kim SH
AD  - College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea.
LA  - eng
GR  - 2017R1A2A1A17069297/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20200409
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC7235876
OTO - NOTNLM
OT  - DR5
OT  - EGCG
OT  - TRAIL
OT  - apoptosis
OT  - caspase
OT  - colon cancer
COIS- The authors declare no conflict of interest.
EDAT- 2020/04/15 06:00
MHDA- 2020/04/15 06:01
PMCR- 2020/04/09
CRDT- 2020/04/15 06:00
PHST- 2020/03/25 00:00 [received]
PHST- 2020/04/07 00:00 [revised]
PHST- 2020/04/08 00:00 [accepted]
PHST- 2020/04/15 06:00 [entrez]
PHST- 2020/04/15 06:00 [pubmed]
PHST- 2020/04/15 06:01 [medline]
PHST- 2020/04/09 00:00 [pmc-release]
AID - biomedicines8040084 [pii]
AID - biomedicines-08-00084 [pii]
AID - 10.3390/biomedicines8040084 [doi]
PST - epublish
SO  - Biomedicines. 2020 Apr 9;8(4):84. doi: 10.3390/biomedicines8040084.