PMID- 32289378
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1096-1194 (Electronic)
IS  - 0890-8508 (Linking)
VI  - 52
DP  - 2020 Aug
TI  - MicroRNA-330-3p represses the proliferation and invasion of laryngeal squamous 
      cell carcinoma through downregulation of Tra2beta-mediated Akt signaling.
PG  - 101574
LID - S0890-8508(20)30151-1 [pii]
LID - 10.1016/j.mcp.2020.101574 [doi]
AB  - MicroRNAs (miRNAs), a type of post-transcriptional regulators, exert a crucial 
      role in the malignant progression of laryngeal squamous cell carcinoma (LSCC). 
      MicroRNA-330-3p (miR-330-3p), a recently identified tumor-associated miRNA, is 
      implicated in multiple cancers. Yet, the relevance of miR-330-3p in LSCC remains 
      unexplored. The findings of our study demonstrated a lower expression of 
      miR-330-3p in LSCC. Functional assays revealed that upregulation of miR-330-3p 
      marked restricted the proliferation, colony formation and invasion of LSCC cells. 
      Transformer-2 protein homolog beta (Tra2beta) was identified as a target gene of 
      miR-330-3p. An inverse correlation between miR-330-3p and Tra2beta mRNA expression 
      was evidenced in LSCC specimens. The upregulation of miR-330-3p significantly 
      repressed Tra2beta expression and the phosphorylation of the Akt protein. In 
      addition, Tra2beta overexpression markedly abrogated the tumor suppressive role of 
      miR-330-3p in LSCC cells. Overall, our results uncovered that miR-330-3p exerted 
      a tumor-inhibition function in LSCC through targeting Tra2beta to inhibit Akt 
      activation.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Cheng, Ying
AU  - Cheng Y
AD  - The Department of Otolaryngology Head and Neck Surgery, The Second Affiliated 
      Hospital of Xi'an Jiaotong University, Xi'an, 710004, China.
FAU - Zhu, Hui
AU  - Zhu H
AD  - The Department of Computer, Xi'an University of Post and Telecommunications, 
      Xi'an, 710121, China.
FAU - Gao, Wei
AU  - Gao W
AD  - The Department of Chest Surgery, Shaanxi Provincial Tumor Hospital, Xi'an, 
      710061, China. Electronic address: gao_weigreat@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200411
PL  - England
TA  - Mol Cell Probes
JT  - Molecular and cellular probes
JID - 8709751
RN  - 0 (MIRN330 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (TRA2B protein, human)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Down-Regulation/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/*pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - Models, Biological
MH  - Neoplasm Invasiveness
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - Serine-Arginine Splicing Factors/*genetics/metabolism
MH  - *Signal Transduction/genetics
OTO - NOTNLM
OT  - Akt
OT  - LSCC
OT  - Tra2beta
OT  - miR-330-3p
COIS- Declaration of competing interest The authors declare no conflict of interest.
EDAT- 2020/04/15 06:00
MHDA- 2021/06/03 06:00
CRDT- 2020/04/15 06:00
PHST- 2020/03/08 00:00 [received]
PHST- 2020/04/09 00:00 [revised]
PHST- 2020/04/09 00:00 [accepted]
PHST- 2020/04/15 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/04/15 06:00 [entrez]
AID - S0890-8508(20)30151-1 [pii]
AID - 10.1016/j.mcp.2020.101574 [doi]
PST - ppublish
SO  - Mol Cell Probes. 2020 Aug;52:101574. doi: 10.1016/j.mcp.2020.101574. Epub 2020 
      Apr 11.