PMID- 32291289
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 2160-1836 (Electronic)
IS  - 2160-1836 (Linking)
VI  - 10
IP  - 6
DP  - 2020 Jun 1
TI  - An NCBP3-Domain Protein Mediates Meiotic Silencing by Unpaired DNA.
PG  - 1919-1927
LID - 10.1534/g3.120.401236 [doi]
AB  - In the filamentous fungus Neurospora crassa, genes unpaired during meiosis are 
      silenced by a process known as meiotic silencing by unpaired DNA (MSUD). MSUD 
      utilizes common RNA interference (RNAi) proteins, such as Dicer and Argonaute, to 
      target homologous mRNAs for silencing. Previously, we demonstrated that nuclear 
      cap-binding proteins NCBP1 and NCBP2 are involved in MSUD. We report here that 
      SAD-8, a protein similar to human NCBP3, also mediates silencing. Although SAD-8 
      is not essential for either vegetative or sexual development, it is required for 
      MSUD. SAD-8 localizes predominantly in the nucleus and interacts with both NCBP1 
      and NCBP2. Similar to NCBP1 and NCBP2, SAD-8 interacts with a component 
      (Argonaute) of the perinuclear meiotic silencing complex (MSC), further 
      implicating the involvement of cap-binding proteins in silencing.
CI  - Copyright (c) 2020 Boone et al.
FAU - Boone, Erin C
AU  - Boone EC
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Xiao, Hua
AU  - Xiao H
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Vierling, Michael M
AU  - Vierling MM
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Decker, Logan M
AU  - Decker LM
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Sy, Victor T
AU  - Sy VT
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Kennedy, Rana F
AU  - Kennedy RF
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Bonham, Marilyn A
AU  - Bonham MA
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Schmitz, Shannon F
AU  - Schmitz SF
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - John, Annie M
AU  - John AM
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and.
FAU - Hammond, Thomas M
AU  - Hammond TM
AD  - School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
FAU - Shiu, Patrick K T
AU  - Shiu PKT
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 
      and shiup@missouri.edu.
LA  - eng
GR  - P01 GM068087/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200601
PL  - England
TA  - G3 (Bethesda)
JT  - G3 (Bethesda, Md.)
JID - 101566598
RN  - 0 (DNA, Fungal)
RN  - 0 (Fungal Proteins)
SB  - IM
MH  - DNA, Fungal
MH  - Fungal Proteins/genetics
MH  - *Gene Silencing
MH  - Humans
MH  - Meiosis
MH  - *Neurospora crassa/genetics/metabolism
PMC - PMC7263685
OTO - NOTNLM
OT  - Neurospora crassa
OT  - RNA interference (RNAi)
OT  - cap-binding proteins (CBPs)
OT  - meiosis
OT  - meiotic silencing by unpaired DNA (MSUD)
EDAT- 2020/04/16 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/04/14
CRDT- 2020/04/16 06:00
PHST- 2020/04/16 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/04/16 06:00 [entrez]
PHST- 2020/04/14 00:00 [pmc-release]
AID - g3.120.401236 [pii]
AID - GGG_401236 [pii]
AID - 10.1534/g3.120.401236 [doi]
PST - epublish
SO  - G3 (Bethesda). 2020 Jun 1;10(6):1919-1927. doi: 10.1534/g3.120.401236.