PMID- 32291342
OWN - NLM
STAT- MEDLINE
DCOM- 20200825
LR  - 20200825
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 117
IP  - 17
DP  - 2020 Apr 28
TI  - A common coupling mechanism for A-type heme-copper oxidases from bacteria to 
      mitochondria.
PG  - 9349-9355
LID - 10.1073/pnas.2001572117 [doi]
AB  - Mitochondria metabolize almost all the oxygen that we consume, reducing it to 
      water by cytochrome c oxidase (CcO). CcO maximizes energy capture into the 
      protonmotive force by pumping protons across the mitochondrial inner membrane. 
      Forty years after the H(+)/e(-) stoichiometry was established, a consensus has 
      yet to be reached on the route taken by pumped protons to traverse CcO's 
      hydrophobic core and on whether bacterial and mitochondrial CcOs operate via the 
      same coupling mechanism. To resolve this, we exploited the unique amenability to 
      mitochondrial DNA mutagenesis of the yeast Saccharomyces cerevisiae to introduce 
      single point mutations in the hydrophilic pathways of CcO to test function. From 
      adenosine diphosphate to oxygen ratio measurements on preparations of intact 
      mitochondria, we definitely established that the D-channel, and not the 
      H-channel, is the proton pump of the yeast mitochondrial enzyme, supporting an 
      identical coupling mechanism in all forms of the enzyme.
CI  - Copyright (c) 2020 the Author(s). Published by PNAS.
FAU - Marechal, Amandine
AU  - Marechal A
AUID- ORCID: 0000-0003-3460-3806
AD  - Department of Biological Sciences, Birkbeck College, WC1E 7HX London, United 
      Kingdom; a.marechal@ucl.ac.uk prr@ucl.ac.uk.
AD  - Department of Structural and Molecular Biology, University College London, WC1E 
      6BT London, United Kingdom.
FAU - Xu, Jing-Yang
AU  - Xu JY
AUID- ORCID: 0000-0002-5561-6385
AD  - Department of Structural and Molecular Biology, University College London, WC1E 
      6BT London, United Kingdom.
FAU - Genko, Naho
AU  - Genko N
AD  - Department of Structural and Molecular Biology, University College London, WC1E 
      6BT London, United Kingdom.
FAU - Hartley, Andrew M
AU  - Hartley AM
AUID- ORCID: 0000-0001-7986-6736
AD  - Department of Biological Sciences, Birkbeck College, WC1E 7HX London, United 
      Kingdom.
FAU - Haraux, Francis
AU  - Haraux F
AD  - Universite Paris-Saclay, Commissariat a l'energie atomique et aux energies 
      alternatives, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 
      Gif-sur-Yvette, France.
FAU - Meunier, Brigitte
AU  - Meunier B
AUID- ORCID: 0000-0002-6988-4663
AD  - Universite Paris-Saclay, Commissariat a l'energie atomique et aux energies 
      alternatives, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 
      Gif-sur-Yvette, France.
FAU - Rich, Peter R
AU  - Rich PR
AUID- ORCID: 0000-0001-7756-4718
AD  - Department of Structural and Molecular Biology, University College London, WC1E 
      6BT London, United Kingdom; a.marechal@ucl.ac.uk prr@ucl.ac.uk.
LA  - eng
GR  - MR/M00936X/1/MRC_/Medical Research Council/United Kingdom
GR  - BB/K001094/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/L020165/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200414
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Proton Pumps)
RN  - 0 (Protons)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 42VZT0U6YR (Heme)
RN  - 789U1901C5 (Copper)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.16.- (copper oxidase)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Bacteria/metabolism
MH  - Copper/chemistry/metabolism
MH  - Electron Transport Complex IV/*chemistry/genetics/metabolism
MH  - Heme/*chemistry
MH  - Ion Transport
MH  - Mitochondria/metabolism
MH  - Mitochondrial Membranes/metabolism
MH  - Oxidation-Reduction
MH  - Oxidoreductases/*chemistry/metabolism
MH  - Oxygen/metabolism
MH  - Proton Pumps/metabolism
MH  - Protons
MH  - Saccharomyces cerevisiae/metabolism
MH  - Saccharomyces cerevisiae Proteins/metabolism
PMC - PMC7196763
OTO - NOTNLM
OT  - ADP/O ratio
OT  - H/e stoichiometry
OT  - cytochrome c oxidase
OT  - mitochondria
OT  - proton pumping
COIS- The authors declare no competing interest.
EDAT- 2020/04/16 06:00
MHDA- 2020/08/26 06:00
PMCR- 2020/04/14
CRDT- 2020/04/16 06:00
PHST- 2020/04/16 06:00 [pubmed]
PHST- 2020/08/26 06:00 [medline]
PHST- 2020/04/16 06:00 [entrez]
PHST- 2020/04/14 00:00 [pmc-release]
AID - 2001572117 [pii]
AID - 202001572 [pii]
AID - 10.1073/pnas.2001572117 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9349-9355. doi: 
      10.1073/pnas.2001572117. Epub 2020 Apr 14.