PMID- 32293137
OWN - NLM
STAT- MEDLINE
DCOM- 20210122
LR  - 20210122
IS  - 2366-7478 (Print)
IS  - 2366-7478 (Linking)
VI  - 4
IP  - 2
DP  - 2020 Feb
TI  - Evaluation of Bystander Infection of Oncolytic Virus using a Medium Flow 
      Integrated 3D In Vitro Microphysiological System.
PG  - e1900143
LID - 10.1002/adbi.201900143 [doi]
AB  - Replicable oncolytic viruses (OVs) induce tumor cell lysis and release viral 
      progeny. The released progeny virions and cell debris can spread within 
      surrounding tumor cells or blood vessels. These released molecules may also 
      induce bystander damage in additional tumor cells through spreading within 
      surrounding tumor cells or blood vessels. However, this effect has not been 
      clearly demonstrated due to the difficulty of direct observation. Here, the 
      bystander infection of OVs by vessel delivery and selective infection in 3D 
      multicellular tumoroids (MCTs) in an in vitro microphysiological system (MPS) 
      with integrated medium flow is demonstrated. This study uses replicable vesicular 
      stomatitis virus (VSV)-green fluorescence protein (GFP) to identify the location 
      of infection in 3D MCTs. Using this MPS, the oncoselective infection by VSV-GFP 
      and the spreading by delivery of OVs through flow via block-to-block linkage of 
      the primary infected MPS with uninfected 3D MCTs in an integrated MPS is 
      observed. This MPS enables real-time monitoring and various analysis for the 
      bystander infection of OVs. It is expected that the 3D in vitro MPS can be 
      suitable to investigate the oncoselective spreading and bystander infection of 
      OVs.
CI  - (c) 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Lee, Sang Woo
AU  - Lee SW
AUID- ORCID: 0000-0001-9913-0638
AD  - Biomedical Engineering Research Center, Asan Institute for Life Science, Asan 
      Medical Center, Seoul, 05505, Korea.
FAU - Lee, Kyoung Jin
AU  - Lee KJ
AD  - Department of Microbiology, University of Ulsan College of Medicine, Seoul, 
      05505, Korea.
AD  - Bio-Medical Institute of Technology, Asan Medical Center, Seoul, 05505, Korea.
FAU - Jeong, Soo Yeon
AU  - Jeong SY
AD  - Biomedical Engineering Research Center, Asan Institute for Life Science, Asan 
      Medical Center, Seoul, 05505, Korea.
FAU - Joo, Chul Hyun
AU  - Joo CH
AD  - Department of Microbiology, University of Ulsan College of Medicine, Seoul, 
      05505, Korea.
AD  - Bio-Medical Institute of Technology, Asan Medical Center, Seoul, 05505, Korea.
FAU - Lee, Heuiran
AU  - Lee H
AD  - Department of Microbiology, University of Ulsan College of Medicine, Seoul, 
      05505, Korea.
AD  - Bio-Medical Institute of Technology, Asan Medical Center, Seoul, 05505, Korea.
FAU - Jeong, Gi Seok
AU  - Jeong GS
AUID- ORCID: 0000-0002-2584-9129
AD  - Biomedical Engineering Research Center, Asan Institute for Life Science, Asan 
      Medical Center, Seoul, 05505, Korea.
AD  - Department of Convergence Medicine, University of Ulsan College of Medicine, 
      Seoul, 05505, Korea.
LA  - eng
GR  - NRF-2019R1A2C2005244/National Research Foundation of Korea/International
GR  - NRF-2019R1C1C1007468/National Research Foundation of Korea/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191211
PL  - Germany
TA  - Adv Biosyst
JT  - Advanced biosystems
JID - 101711718
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
MH  - A549 Cells
MH  - Cells, Cultured
MH  - *Cytological Techniques/instrumentation/methods
MH  - Equipment Design
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - *Models, Biological
MH  - Oncolytic Virotherapy/*adverse effects
MH  - *Oncolytic Viruses/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Rhabdoviridae Infections/virology
MH  - Spheroids, Cellular/virology
MH  - Tumor Cells, Cultured/virology
MH  - Vesiculovirus/genetics
OTO - NOTNLM
OT  - drug discovery
OT  - microfluidic platform
OT  - microphysiological system
OT  - oncolytic virus
OT  - preclinical study
EDAT- 2020/04/16 06:00
MHDA- 2021/01/23 06:00
CRDT- 2020/04/16 06:00
PHST- 2019/07/08 00:00 [received]
PHST- 2019/10/04 00:00 [revised]
PHST- 2020/04/16 06:00 [entrez]
PHST- 2020/04/16 06:00 [pubmed]
PHST- 2021/01/23 06:00 [medline]
AID - 10.1002/adbi.201900143 [doi]
PST - ppublish
SO  - Adv Biosyst. 2020 Feb;4(2):e1900143. doi: 10.1002/adbi.201900143. Epub 2019 Dec 
      11.