PMID- 32293200
OWN - NLM
STAT- MEDLINE
DCOM- 20201102
LR  - 20210105
IS  - 1552-9924 (Electronic)
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 128
IP  - 4
DP  - 2020 Apr
TI  - Neuroinflammatory and Behavioral Outcomes Measured in Adult Offspring of Mice 
      Exposed Prenatally to E-Cigarette Aerosols.
PG  - 47006
LID - 10.1289/EHP6067 [doi]
LID - 047006
AB  - BACKGROUND: In an effort to decrease the rates of smoking conventional tobacco 
      cigarettes, electronic cigarettes (e-cigarettes) have been proposed as an 
      effective smoking cessation tool. However, little is known about their 
      toxicological impacts. This is concerning given that e-cigarette use is perceived 
      as less harmful than conventional tobacco cigarettes during pregnancy for both 
      the mother and fetus. OBJECTIVE: The goal of this study was to test the 
      neurodevelopmental consequences of maternal e-cigarette use on adult offspring 
      behavior and neuroimmune outcomes. METHODS: Pregnant female CD-1 mice were 
      randomly assigned to one of three treatment groups (n = 8 - 10 per group) and 
      exposed daily to either filtered air, propylene glycol and vegetable glycerol 
      (50:50 PG/VG vehicle), or to PG/VG with 16 mg/mL nicotine ( + Nic). Whole-body 
      exposures were carried out for 3 h/d, 7 d/week, from gestational day (GD)0.5 
      until GD17.5. Adult male and female offspring (8 weeks old) were assessed across 
      a battery of behavioral assessments followed by region-specific quantification of 
      brain cytokines using multiplex immunoassays. RESULTS: Adult offspring of both 
      sexes exposed to + Nic exhibited elevated locomotor activity in the elevated plus 
      maze and altered stress-coping strategies in the forced swim task. Moreover, male 
      and female offspring exposed to PG/VG with and without nicotine had a 5.2% lower 
      object discrimination score in the novel object recognition task. In addition to 
      differences in offspring behavior, maternal e-cigarette exposure with nicotine 
      led to a reduction in interleukin (IL)-4 and interferon-gamma (IFNgamma) in the 
      diencephalon, as well as lower levels of hippocampal IFNgamma (females only). 
      E-cigarette exposure without nicotine resulted in a 2-fold increase of IL-6 in 
      the cerebellum. DISCUSSION: These findings support previous adverse findings of 
      e-cigarette exposure on neurodevelopment in a mouse model and provide substantial 
      evidence of persistent adverse behavioral and neuroimmunological consequences to 
      adult offspring following maternal e-cigarette exposure during pregnancy. 
      https://doi.org/10.1289/EHP6067.
FAU - Church, Jamie S
AU  - Church JS
AD  - Program in Neuroscience and Behavior, Department of Psychology and Education, 
      Mount Holyoke College, South Hadley, Massachusetts, USA.
FAU - Chace-Donahue, Fiona
AU  - Chace-Donahue F
AD  - Program in Neuroscience and Behavior, Department of Psychology and Education, 
      Mount Holyoke College, South Hadley, Massachusetts, USA.
FAU - Blum, Jason L
AU  - Blum JL
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, New York, USA.
FAU - Ratner, Jill R
AU  - Ratner JR
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, New York, USA.
FAU - Zelikoff, Judith T
AU  - Zelikoff JT
AD  - Department of Environmental Medicine, New York University School of Medicine, New 
      York, New York, USA.
FAU - Schwartzer, Jared J
AU  - Schwartzer JJ
AD  - Program in Neuroscience and Behavior, Department of Psychology and Education, 
      Mount Holyoke College, South Hadley, Massachusetts, USA.
LA  - eng
GR  - P30 ES000260/ES/NIEHS NIH HHS/United States
GR  - R15 HD082638/HD/NICHD NIH HHS/United States
GR  - R15 MH119500/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20200415
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Aerosols)
RN  - 6DC9Q167V3 (Propylene Glycol)
RN  - 6M3C89ZY6R (Nicotine)
RN  - PDC6A3C0OX (Glycerol)
SB  - IM
CIN - Environ Health Perspect. 2020 Oct;128(10):104005. PMID: 33104447
MH  - Aerosols/analysis
MH  - Animals
MH  - Disease Models, Animal
MH  - *Electronic Nicotine Delivery Systems
MH  - Female
MH  - Glycerol/adverse effects
MH  - Inflammation/chemically induced/*immunology
MH  - Locomotion/*drug effects
MH  - Mice
MH  - Nicotine/adverse effects
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/chemically induced/*immunology/*psychology
MH  - Propylene Glycol/adverse effects
MH  - Random Allocation
MH  - Stress, Psychological/chemically induced/*psychology
PMC - PMC7228099
EDAT- 2020/04/16 06:00
MHDA- 2020/11/03 06:00
PMCR- 2020/04/15
CRDT- 2020/04/16 06:00
PHST- 2020/04/16 06:00 [entrez]
PHST- 2020/04/16 06:00 [pubmed]
PHST- 2020/11/03 06:00 [medline]
PHST- 2020/04/15 00:00 [pmc-release]
AID - EHP6067 [pii]
AID - 10.1289/EHP6067 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2020 Apr;128(4):47006. doi: 10.1289/EHP6067. Epub 2020 
      Apr 15.