PMID- 32294897
OWN - NLM
STAT- MEDLINE
DCOM- 20210112
LR  - 20210112
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 12
IP  - 4
DP  - 2020 Apr 13
TI  - First-degree Relatives of Celiac Disease Patients Have Increased Seroreactivity 
      to Serum Microbial Markers.
LID - 10.3390/nu12041073 [doi]
LID - 1073
AB  - Risk of celiac disease (CD) is increased in relatives of CD patients due to 
      genetic and possible environmental factors. We recently reported increased 
      seropositivity to anti-Saccharomyces cerevisiae (ASCA), Pseudomonas 
      fluorescens-associated sequence (anti-I2) and Bacteroides caccae TonB-linked 
      outer membrane protein (anti-OmpW) antibodies in CD. We hypothesized these 
      markers also to be overrepresented in relatives. Seropositivity and levels of 
      ASCA, anti-I2 and anti-OmpW were compared between 463 first-degree relatives, 58 
      untreated and 55 treated CD patients, and 80 controls. CD-associated human 
      leukocyte antigen (HLA)-haplotypes and transglutaminase (tTGab) and endomysium 
      (EmA) antibodies were determined. One or more of the microbial antibodies was 
      present in 75% of relatives, 97% of untreated and 87% of treated CD patients and 
      44% of the controls. The relatives had higher median ASCA IgA (9.13 vs. 4.50 
      U/mL, p < 0.001), ASCA IgG (8.91 vs. 5.75 U/mL, p < 0.001) and anti-I2 
      (absorbance 0.74 vs. 0.32, p < 0.001) levels than controls. There was a weak, 
      positive correlation between tTGab and ASCA (r = 0.31, p < 0.001). Seropositivity 
      was not significantly associated with HLA. To conclude, seropositivity to 
      microbial markers was more common and ASCA and anti-I2 levels higher in relatives 
      of CD patients than controls. These findings were not associated with HLA, 
      suggesting the role of other genetic and environmental factors.
FAU - Viitasalo, Liisa
AU  - Viitasalo L
AD  - Centre for Child Health Research, Tampere University, and Department of 
      Pediatrics, Tampere University Hospital, FI-33521 Tampere, Finland.
AD  - Laboratory of Genetics, HUS Diagnostic Center, Helsinki University Hospital, 
      FI-00029 Helsinki, Finland.
FAU - Iltanen, Sari
AU  - Iltanen S
AD  - Centre for Child Health Research, Tampere University, and Department of 
      Pediatrics, Tampere University Hospital, FI-33521 Tampere, Finland.
AD  - Lapland Central Hospital, FI-96101 Rovaniemi, Finland.
FAU - Huhtala, Heini
AU  - Huhtala H
AD  - Faculty of Social Sciences, Tampere University, FI-33520 Tampere, Finland.
FAU - Saavalainen, Paivi
AU  - Saavalainen P
AD  - Research Program Unit, Immunobiology, and Department of Medical and Clinical 
      Genetics, University of Helsinki, FI-00014 Helsinki, Finland.
FAU - Kaukinen, Katri
AU  - Kaukinen K
AD  - Celiac Disease Research Centre, Faculty of Medicine and Health Technology, 
      Tampere University, FI-33520 Tampere, Finland.
AD  - Department of Internal Medicine, Tampere University Hospital, FI-33521 Tampere, 
      Finland.
FAU - Lindfors, Katri
AU  - Lindfors K
AD  - Celiac Disease Research Centre, Faculty of Medicine and Health Technology, 
      Tampere University, FI-33520 Tampere, Finland.
FAU - Kurppa, Kalle
AU  - Kurppa K
AD  - Centre for Child Health Research, Tampere University, and Department of 
      Pediatrics, Tampere University Hospital, FI-33521 Tampere, Finland.
AD  - Department of Pediatrics, Seinajoki University Hospital and the University 
      Consortium of Seinajoki, FI-60320 Seinajoki, Finland.
LA  - eng
GR  - NA/Academy of Finland/
GR  - NA/the Finnish Medical Foundation/
GR  - NA/Paivikki ja Sakari Sohlbergin Saatio/
GR  - NA/the Paulo Foundation/
GR  - NA/Sigrid Juseliuksen Saatio/
GR  - NA/the Foundation for Pediatric Research/
GR  - NA/the Competitive State Research Financing of the Expert Area of Tampere 
      University Hospital/
PT  - Journal Article
DEP - 20200413
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antibodies, Fungal)
RN  - 0 (HLA Antigens)
RN  - 0 (Immunoglobulin A)
RN  - EC 2.3.2.13 (Transglutaminases)
SB  - IM
MH  - Adult
MH  - Antibodies, Bacterial/*blood
MH  - Antibodies, Fungal/*blood
MH  - Celiac Disease/*genetics/*immunology
MH  - *Family
MH  - Female
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Immunoglobulin A/blood
MH  - Male
MH  - Middle Aged
MH  - Pseudomonas fluorescens/immunology
MH  - Saccharomyces cerevisiae/immunology
MH  - Transglutaminases/immunology
PMC - PMC7230150
OTO - NOTNLM
OT  - Bacteroides caccae
OT  - Pseudomonas fluorescens
OT  - Saccharomyces cerevisiae
OT  - celiac disease
OT  - microbiota
OT  - relatives
COIS- The authors declare no conflicts of interest.
EDAT- 2020/04/17 06:00
MHDA- 2021/01/13 06:00
PMCR- 2020/04/01
CRDT- 2020/04/17 06:00
PHST- 2020/03/23 00:00 [received]
PHST- 2020/04/02 00:00 [revised]
PHST- 2020/04/08 00:00 [accepted]
PHST- 2020/04/17 06:00 [entrez]
PHST- 2020/04/17 06:00 [pubmed]
PHST- 2021/01/13 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - nu12041073 [pii]
AID - nutrients-12-01073 [pii]
AID - 10.3390/nu12041073 [doi]
PST - epublish
SO  - Nutrients. 2020 Apr 13;12(4):1073. doi: 10.3390/nu12041073.