PMID- 32295270
OWN - NLM
STAT- MEDLINE
DCOM- 20201216
LR  - 20201216
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 10
IP  - 4
DP  - 2020 Apr 14
TI  - Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled 
      Monolayers Modified ITO Electrode.
LID - 10.3390/bios10040038 [doi]
LID - 38
AB  - The immunoglobulin E (IgE) level in serum is an important factor in the 
      examination of allergy. Ferrocene (Fc)-modified self-assembled monolayers (SAMs) 
      were placed on an indium tin oxide (ITO) electrode as a sensing layer for the 
      detection of human IgE. The Fc moiety in the SAMs facilitated the electron 
      transfer through the organic SAMs layer and electrocatalytic signal 
      amplification. The electrochemical measurement was accomplished after the 
      sandwich type immobilization of the receptor antibody, target human IgE, and 
      enzyme conjugated secondary antibody. The enzyme product, p-aminophenol, was 
      quantitatively analyzed by redox cycling via Fc. In addition, the electrochemical 
      impedance spectroscopy (EIS) was investigated for the detection of IgE. The limit 
      of detection (LOD), limit of quantification (LOQ), and dynamic range of the 
      electrochemical sensor were 3 IU/mL, 10 IU/mL, and from 10 IU/mL to 100 IU/mL, 
      respectively.
FAU - Park, Myungsang
AU  - Park M
AD  - Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 
      05029, Korea.
FAU - Song, Yesol
AU  - Song Y
AD  - Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 
      05029, Korea.
FAU - Kim, Ki Jun
AU  - Kim KJ
AD  - Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 
      05029, Korea.
FAU - Oh, Seung Jun
AU  - Oh SJ
AD  - Pioneering Research Center, LG Chem. Ltd., 30 Magokjungang 10-ro, Gangseo-gu, 
      Seoul 07796, Korea.
FAU - Ahn, Jun Ki
AU  - Ahn JK
AD  - Pioneering Research Center, LG Chem. Ltd., 30 Magokjungang 10-ro, Gangseo-gu, 
      Seoul 07796, Korea.
AD  - Human Convergence Technology Group, Korea Institute of Industrial Technology 
      (KITECH), 143 Hanggaul-ro, Sangnok-gu, Ansan 15588, Korea.
FAU - Park, Hun
AU  - Park H
AD  - Pioneering Research Center, LG Chem. Ltd., 30 Magokjungang 10-ro, Gangseo-gu, 
      Seoul 07796, Korea.
FAU - Shin, Hang-Beum
AU  - Shin HB
AUID- ORCID: 0000-0002-2212-4122
AD  - Pioneering Research Center, LG Chem. Ltd., 30 Magokjungang 10-ro, Gangseo-gu, 
      Seoul 07796, Korea.
FAU - Kwon, Seong Jung
AU  - Kwon SJ
AD  - Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 
      05029, Korea.
LA  - eng
GR  - 2018R1A2B6009591/National Research Foundation of Korea/
GR  - 2018/Konkuk University/
PT  - Journal Article
DEP - 20200414
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - 0 (Ferrous Compounds)
RN  - 0 (Metallocenes)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - U96PKG90JQ (ferrocene)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Electrochemistry/*methods
MH  - Electrodes
MH  - Ferrous Compounds/*chemistry
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Metallocenes/*chemistry
PMC - PMC7235775
OTO - NOTNLM
OT  - electrocatalytic reaction
OT  - electrochemical impedance spectroscopy
OT  - ferrocene
OT  - human immunoglobulin E
OT  - indium tin oxide (ITO)
COIS- The authors declare no conflict of interest.
EDAT- 2020/04/17 06:00
MHDA- 2020/12/17 06:00
PMCR- 2020/04/01
CRDT- 2020/04/17 06:00
PHST- 2020/03/12 00:00 [received]
PHST- 2020/04/10 00:00 [revised]
PHST- 2020/04/12 00:00 [accepted]
PHST- 2020/04/17 06:00 [entrez]
PHST- 2020/04/17 06:00 [pubmed]
PHST- 2020/12/17 06:00 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - bios10040038 [pii]
AID - biosensors-10-00038 [pii]
AID - 10.3390/bios10040038 [doi]
PST - epublish
SO  - Biosensors (Basel). 2020 Apr 14;10(4):38. doi: 10.3390/bios10040038.