PMID- 32296115
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20210415
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Apr 15
TI  - Preserving Airway Smooth Muscle Contraction in Precision-Cut Lung Slices.
PG  - 6480
LID - 10.1038/s41598-020-63225-y [doi]
LID - 6480
AB  - Precision-cut lung slices (PCLS) are ideal for measuring small airway 
      contraction. However, these measurements are currently limited to acute exposure 
      scenarios that typically last a few minutes to a few hours. Using an 
      insulin-supplemented culture medium, we prolong the small airway contractility in 
      mouse PCLS for up to two weeks. Compared to conventional culture medium, 
      insulin-supplemented culture medium provides no additional benefit in preserving 
      cellular viability or airway structure. However, it protects the airway smooth 
      muscle (ASM) against a loss of smooth muscle myosin heavy chain (SMMHC) 
      expression. We elucidate the significance of this new culture medium for chronic 
      disease modeling of IL-13-induced airway hyper-responsiveness.
FAU - Li, Guang
AU  - Li G
AD  - Department of Critical Care Medicine, Renmin Hospital and Wuhan University, Wu 
      Han, 430071, China.
FAU - Cohen, Jonathan A
AU  - Cohen JA
AD  - Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard 
      Medical School, Boston, MA, 02115, USA.
FAU - Martines, Carolina
AU  - Martines C
AD  - Molecular and Integrative Physiological Sciences Program, Department of 
      Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, 
      02115, USA.
FAU - Ram-Mohan, Sumati
AU  - Ram-Mohan S
AD  - Center for Vascular Biology Research, Department of Emergency Medicine, Beth 
      Israel Deaconess Medical Center, Boston, MA, 02215, USA.
FAU - Brain, Joseph D
AU  - Brain JD
AD  - Molecular and Integrative Physiological Sciences Program, Department of 
      Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, 
      02115, USA.
FAU - Krishnan, Ramaswamy
AU  - Krishnan R
AD  - Center for Vascular Biology Research, Department of Emergency Medicine, Beth 
      Israel Deaconess Medical Center, Boston, MA, 02215, USA.
FAU - Ai, Xingbin
AU  - Ai X
AD  - Department of Pediatrics, Massachusetts General Hospital and Harvard Medical 
      School, Boston, Boston, MA, 02114, USA.
FAU - Bai, Yan
AU  - Bai Y
AD  - Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard 
      Medical School, Boston, MA, 02115, USA. ybai4@bwh.harvard.edu.
LA  - eng
GR  - K08 HL135443/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20200415
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Culture Media)
RN  - 0 (Insulin)
RN  - 0 (Interleukin-13)
RN  - EC 3.6.4.1 (Myosin Heavy Chains)
SB  - IM
MH  - Animals
MH  - Culture Media/metabolism
MH  - Female
MH  - Humans
MH  - Insulin/metabolism
MH  - Interleukin-13/immunology/metabolism
MH  - Lung/immunology/*physiopathology
MH  - Male
MH  - Mice
MH  - Muscle Contraction/*physiology
MH  - Muscle, Smooth/cytology/*physiology
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Myosin Heavy Chains/metabolism
MH  - Respiratory Hypersensitivity/*immunology/physiopathology
MH  - Tissue Culture Techniques/*methods
PMC - PMC7160136
COIS- The authors declare no competing interests.
EDAT- 2020/04/17 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/04/15
CRDT- 2020/04/17 06:00
PHST- 2019/12/23 00:00 [received]
PHST- 2020/03/21 00:00 [accepted]
PHST- 2020/04/17 06:00 [entrez]
PHST- 2020/04/17 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/04/15 00:00 [pmc-release]
AID - 10.1038/s41598-020-63225-y [pii]
AID - 63225 [pii]
AID - 10.1038/s41598-020-63225-y [doi]
PST - epublish
SO  - Sci Rep. 2020 Apr 15;10(1):6480. doi: 10.1038/s41598-020-63225-y.