PMID- 32296417
OWN - NLM
STAT- MEDLINE
DCOM- 20210304
LR  - 20210304
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 11
DP  - 2020
TI  - Tissue-Specific Factors Differentially Regulate the Expression of 
      Antigen-Processing Enzymes During Dendritic Cell Ontogeny.
PG  - 453
LID - 10.3389/fimmu.2020.00453 [doi]
LID - 453
AB  - Dendritic cells (DCs) form a collection of antigen-presenting cells (APCs) that 
      are distributed throughout the body. Conventional DCs (cDCs), which include the 
      cDC1 and cDC2 subsets, and plasmacytoid DCs (pDCs) constitute the two major 
      ontogenically distinct DC populations. The pDCs complete their differentiation in 
      the bone marrow (BM), whereas the cDC subsets derive from pre-committed BM 
      precursors, the pre-cDC, that seed lymphoid and non-lymphoid tissues where they 
      further differentiate into mature cDC1 and cDC2. Within different tissues, cDCs 
      express distinct phenotype and function. Notably, cDCs in the thymus are 
      exquisitely efficient at processing and presenting antigens in the class II 
      pathway, whereas in the spleen they do so only upon maturation induced by danger 
      signals. To appraise this functional heterogeneity, we examined the regulation of 
      the expression of distinct antigen-processing enzymes during DC ontogeny. We 
      analyzed the expression of cathepsin S (CTSS), cathepsin L (CTSL), and 
      thymus-specific serine protease (TSSP), three major antigen-processing enzymes 
      regulating class II presentation in cDC, by DC BM precursors and immature and 
      mature cDCs from the spleen and thymus. We found that pre-cDCs in the BM express 
      relatively high levels of these different proteases. Then, their expression is 
      modulated in a tissue-specific and subset-specific manner with immature and 
      mature thymic cDCs expressing overall higher levels than immature splenic cDCs. 
      On the other hand, the TSSP expression level is selectively down-regulated in 
      spleen pDCs, whereas CTSS and CTSL are both increased in thymic and splenic pDCs. 
      Hence, tissue-specific factors program the expression levels of these different 
      proteases during DC differentiation, thus conferring tissue-specific function to 
      the different DC subsets.
CI  - Copyright (c) 2020 Mahiddine, Hassel, Murat, Girard and Guerder.
FAU - Mahiddine, Karim
AU  - Mahiddine K
AD  - Centre de Physiopathologie de Toulouse Purpan, Institut National de la Sante et 
      de la Recherche Medicale, Centre National de la Recherche Scientifique, 
      Universite Paul Sabatier Toulouse III, Toulouse, France.
FAU - Hassel, Chervin
AU  - Hassel C
AD  - Centre de Physiopathologie de Toulouse Purpan, Institut National de la Sante et 
      de la Recherche Medicale, Centre National de la Recherche Scientifique, 
      Universite Paul Sabatier Toulouse III, Toulouse, France.
FAU - Murat, Claire
AU  - Murat C
AD  - Centre de Physiopathologie de Toulouse Purpan, Institut National de la Sante et 
      de la Recherche Medicale, Centre National de la Recherche Scientifique, 
      Universite Paul Sabatier Toulouse III, Toulouse, France.
FAU - Girard, Maeva
AU  - Girard M
AD  - Centre de Physiopathologie de Toulouse Purpan, Institut National de la Sante et 
      de la Recherche Medicale, Centre National de la Recherche Scientifique, 
      Universite Paul Sabatier Toulouse III, Toulouse, France.
FAU - Guerder, Sylvie
AU  - Guerder S
AD  - Centre de Physiopathologie de Toulouse Purpan, Institut National de la Sante et 
      de la Recherche Medicale, Centre National de la Recherche Scientifique, 
      Universite Paul Sabatier Toulouse III, Toulouse, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200331
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Cytokines)
RN  - EC 3.4.- (Cathepsins)
RN  - EC 3.4.- (Serine Proteases)
RN  - EC 3.4.22.15 (Cathepsin L)
RN  - EC 3.4.22.27 (cathepsin S)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Cathepsin L/genetics/*metabolism
MH  - Cathepsins/genetics/*metabolism
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*physiology
MH  - Enzyme Activation
MH  - Gene Expression Regulation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred NOD
MH  - Organ Specificity
MH  - Serine Proteases/genetics/*metabolism
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
MH  - Thymus Gland/*metabolism
PMC - PMC7136460
OTO - NOTNLM
OT  - cathepsin L
OT  - cathepsin S
OT  - dendritic cell ontogeny
OT  - splenic dendritic cell
OT  - thymic dendritic cell
OT  - thymus-specific serine protease
EDAT- 2020/04/17 06:00
MHDA- 2021/03/05 06:00
PMCR- 2020/01/01
CRDT- 2020/04/17 06:00
PHST- 2019/11/02 00:00 [received]
PHST- 2020/02/27 00:00 [accepted]
PHST- 2020/04/17 06:00 [entrez]
PHST- 2020/04/17 06:00 [pubmed]
PHST- 2021/03/05 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2020.00453 [doi]
PST - epublish
SO  - Front Immunol. 2020 Mar 31;11:453. doi: 10.3389/fimmu.2020.00453. eCollection 
      2020.