PMID- 32297689
OWN - NLM
STAT- MEDLINE
DCOM- 20210128
LR  - 20210128
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 55
IP  - 7
DP  - 2020 Jul
TI  - Identification of appropriate reference genes for qPCR analyses of porcine 
      placentae and endometria, supplying foetuses of different size and sex, at 
      multiple gestational days.
PG  - 785-794
LID - 10.1111/rda.13685 [doi]
AB  - Recent studies suggest associations exist between foetal size and sex, and gene 
      expression at the porcine feto-maternal interface. It is essential to identify 
      reference genes which have stable expression throughout gestation in 
      feto-placental units associated with foetuses of different size and sex. qPCR was 
      performed for 11 genes within porcine placentae and endometria at gestational 
      days (GD) 30, 60 and 90. Several reference genes were found to have stable 
      expression in these samples. The combination of B2m1 and Tbp1, and Hprt1 and Tbp1 
      had the most stable expression in endometria and placentae, respectively. 
      Reference genes identified as having stable expression were utilized in a larger 
      experiment with placentae and endometria associated with foetuses of different 
      size and sex at four GD. The average expression of B2m1 and Tbp1 mRNAs was 
      suitable for the normalization of temporal changes in endometria, and comparison 
      between endometria supplying foetuses of different size throughout gestation. The 
      average expression of Hprt1 and Tbp1 mRNAs was suitable for the normalization of 
      placental mRNA expression for comparison of temporal changes and sex differences 
      between placentae supplying foetuses of different sex throughout gestation. This 
      combination was suitable for the normalization of mRNA expression in placentas 
      supplying GD30, GD60 and GD90 foetuses of different size. This study has 
      identified reference genes with stable expression in placentae and endometria 
      across multiple gestational days, in tissues associated with foetuses of 
      different size and sex. The results of these experiments highlight the importance 
      of selecting appropriate reference genes for the biological comparison under 
      investigation.
CI  - (c) 2020 Blackwell Verlag GmbH.
FAU - Stenhouse, Claire
AU  - Stenhouse C
AUID- ORCID: 0000-0001-8302-7900
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Hogg, Charis O
AU  - Hogg CO
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Ashworth, Cheryl J
AU  - Ashworth CJ
AD  - Functional Genetics and Development Division, The Roslin Institute and Royal 
      (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, UK.
LA  - eng
GR  - University Of Edinburgh/
GR  - BB/J004316/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
DEP - 20200512
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Endometrium/*metabolism
MH  - Female
MH  - Fetal Weight
MH  - Gene Expression
MH  - Male
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction/veterinary
MH  - Sus scrofa/physiology
OTO - NOTNLM
OT  - endometrium
OT  - placenta
OT  - porcine
OT  - pregnancy
OT  - reference gene
EDAT- 2020/04/17 06:00
MHDA- 2021/01/29 06:00
CRDT- 2020/04/17 06:00
PHST- 2020/02/18 00:00 [received]
PHST- 2020/03/31 00:00 [revised]
PHST- 2020/04/04 00:00 [accepted]
PHST- 2020/04/17 06:00 [pubmed]
PHST- 2021/01/29 06:00 [medline]
PHST- 2020/04/17 06:00 [entrez]
AID - 10.1111/rda.13685 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2020 Jul;55(7):785-794. doi: 10.1111/rda.13685. Epub 2020 May 
      12.