PMID- 32299765
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1873-4898 (Electronic)
IS  - 1477-5131 (Linking)
VI  - 16
IP  - 3
DP  - 2020 Jun
TI  - Relationship of tumour-associated macrophages with poor prognosis in Wilms' 
      tumour.
PG  - 376.e1-376.e8
LID - S1477-5131(20)30070-X [pii]
LID - 10.1016/j.jpurol.2020.03.016 [doi]
AB  - BACKGROUND: Wilms' tumour (WT) is the most common childhood renal tumour. 
      Tumour-associated macrophages (TAMs) are a critical component of tumour 
      microenvironments and contain two main subtypes, classically (M1) or 
      alternatively (M2) activated macrophages. Evidence has revealed TAMs in 
      predicting poor prognosis in some malignant tumours. However, the role of TAMs in 
      WT is still unclear, and the relationship of different types of TAMs with 
      prognosis has not been elucidated. OBJECTIVE: The aim of the study was to explore 
      the presence of two types of TAMs in WT and analyse the relationship of TAMs with 
      prognosis. STUDY DESIGN: Overall, 61 paediatric patients with WT underwent 
      nephrectomy before any chemotherapy from April 2006 to March 2014. The tumour 
      tissues were analysed by Western blot, immunohistochemistry, and 
      immunofluorescence to explore the distribution of M1 and M2 macrophages in 
      different stages. Kaplan-Meier analysis with regard to the relationship between 
      the presence of TAMs and follow-up information was performed. RESULTS: In the 61 
      patients (44 males and 17 females), there was a median age of 19 months (IQR 
      13-35.5); 47 patients are still alive, 11 died, 3 were lost to follow-up. 
      According to the National Wilms Tumor Study (NWTS)-5 guidelines, the distribution 
      of tumour stages was as follows: stage I, 27 patients; stage II, 18 patients; and 
      stage III, 16 patients. The Western blot analysis showed that the density of M1 
      and M2 macrophages in tumour tissues were significantly greater than that in 
      adjacent normal tissues. Immunohistochemistry showed the proportion of patients 
      with positive M1-type macrophages across different stages: stage I, 66.7% 
      (18/27); stage II, 44.4% (8/18); and stage III, 25% (4/16) (p = 0.027). The 
      proportion of patients with positive M2-type macrophages across different stages: 
      stage I, 25.9% (7/27); stage II, 55.6% (10/18); and stage III, 81.3% (13/16) 
      (p = 0.002). Kaplan-Meier analysis suggested that patients with high densities of 
      M2-type macrophages had shorter overall survival time than those with low 
      densities (log-rank test, p = 0.011). DISCUSSION: TAMs play a pivotal comments in 
      the tumour microenvironment and tumorigenesis. With the progression of clinical 
      stage, M2 macrophage densities increased greatly, and M1 macrophage density 
      decreased. M2 macrophages represent a poor prognosis and can be utilized as a new 
      indicator in pathological examination. CONCLUSION: There is a high density of 
      TAMs in WT, and M2-type macrophage density increases with tumour progression and 
      implies a poor prognosis.
CI  - Copyright (c) 2020 Journal of Pediatric Urology Company. Published by Elsevier Ltd. 
      All rights reserved.
FAU - Tian, Kaixuan
AU  - Tian K
AD  - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong, 250021, PR China.
FAU - Wang, Xiaoqing
AU  - Wang X
AD  - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong, 250021, PR China.
FAU - Wu, Yidi
AU  - Wu Y
AD  - School of Medicine, Shandong University, Jinan, Shandong, 250021, PR China.
FAU - Wu, Xiangyu
AU  - Wu X
AD  - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong, 250021, PR China.
FAU - Du, Guoqiang
AU  - Du G
AD  - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong, 250021, PR China.
FAU - Liu, Wei
AU  - Liu W
AD  - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong, 250021, PR China. Electronic address: 
      lemontree1119@126.com.
FAU - Wu, Rongde
AU  - Wu R
AD  - Department of Pediatric Surgery, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, Shandong, 250021, PR China. Electronic address: 
      shandawrd@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200329
PL  - England
TA  - J Pediatr Urol
JT  - Journal of pediatric urology
JID - 101233150
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Infant
MH  - Male
MH  - Neoplasm Staging
MH  - Prognosis
MH  - Tumor Microenvironment
MH  - *Tumor-Associated Macrophages
MH  - *Wilms Tumor/pathology/therapy
OTO - NOTNLM
OT  - Paediatric
OT  - Prognosis
OT  - Tumour-associated macrophages
OT  - Urology
OT  - Wilms' tumour
COIS- Conflicts of interest The authors declare that there are no conflicts of interest 
      related to this article.
EDAT- 2020/04/18 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/04/18 06:00
PHST- 2019/09/29 00:00 [received]
PHST- 2020/03/18 00:00 [accepted]
PHST- 2020/04/18 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/04/18 06:00 [entrez]
AID - S1477-5131(20)30070-X [pii]
AID - 10.1016/j.jpurol.2020.03.016 [doi]
PST - ppublish
SO  - J Pediatr Urol. 2020 Jun;16(3):376.e1-376.e8. doi: 10.1016/j.jpurol.2020.03.016. 
      Epub 2020 Mar 29.