PMID- 32302339
OWN - NLM
STAT- MEDLINE
DCOM- 20200708
LR  - 20200708
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 15
IP  - 4
DP  - 2020
TI  - Streptococcal H2O2 inhibits IgE-triggered degranulation of RBL-2H3 mast 
      cell/basophil cell line by inducing cell death.
PG  - e0231101
LID - 10.1371/journal.pone.0231101 [doi]
LID - e0231101
AB  - Mast cells and basophils are central players in allergic reactions triggered by 
      immunoglobulin E (IgE). They have intracellular granules containing allergic 
      mediators (e.g., histamine, serotonin, inflammatory cytokines, proteases and 
      beta-hexosaminidase), and stimulation by IgE-allergen complex leads to the release 
      of such allergic mediators from the granules, that is, degranulation. Mast cells 
      are residents of mucosal surfaces, including those of nasal and oral cavities, 
      and play an important role in the innate defense system. Members of the mitis 
      group streptococci such as Streptococcus oralis, are primary colonizers of the 
      human oral cavity. They produce hydrogen peroxide (H2O2) as a by-product of sugar 
      metabolism. In this study, we investigated the effects of streptococcal infection 
      on RBL-2H3 mast cell/basophil cell line. Infection by oral streptococci did not 
      induce degranulation of the cells. Stimulation of the RBL-2H3 cells with 
      anti-dinitrophenol (DNP) IgE and DNP-conjugated human serum albumin triggers 
      degranulation with the release of beta-hexosaminidase. We found that S. oralis and 
      other mitis group streptococci inhibited the IgE-triggered degranulation of 
      RBL-2H3 cells. Since mitis group streptococci produce H2O2, we examined the 
      effect of S. oralis mutant strain deficient in producing H2O2, and found that 
      they lost the ability to suppress the degranulation. Moreover, H2O2 alone 
      inhibited the IgE-induced degranulation. Subsequent analysis suggested that the 
      inhibition of degranulation was related to the cytotoxicity of streptococcal 
      H2O2. Activated RBL-2H3 cells produce interleukin-4 (IL-4); however, IL-4 
      production was not induced by streptococcal H2O2. Furthermore, an in vivo study 
      using the murine pollen-induced allergic rhinitis model suggested that the 
      streptococcal H2O2 reduces nasal allergic reaction. These findings reveal that 
      H2O2 produced by oral mitis group streptococci inhibits IgE-stimulated 
      degranulation by inducing cell death. Consequently, streptococcal H2O2 can be 
      considered to modulate the allergic reaction in mucosal surfaces.
FAU - Okahashi, Nobuo
AU  - Okahashi N
AUID- ORCID: 0000-0002-8045-7021
AD  - Center for Frontier Oral Science, Osaka University Graduate School of Dentistry, 
      Suita, Osaka, Japan.
FAU - Nakata, Masanobu
AU  - Nakata M
AD  - Department of Oral and Molecular Microbiology, Osaka University Graduate School 
      of Dentistry, Suita, Osaka, Japan.
FAU - Hirose, Yujiro
AU  - Hirose Y
AD  - Department of Oral and Molecular Microbiology, Osaka University Graduate School 
      of Dentistry, Suita, Osaka, Japan.
FAU - Morisaki, Hirobumi
AU  - Morisaki H
AD  - Department of Oral Microbiology and Immunology, School of Dentistry, Showa 
      University, Shinagawa, Tokyo, Japan.
FAU - Kataoka, Hideo
AU  - Kataoka H
AD  - Department of Oral Microbiology, Asahi University School of Dentistry, Mizuho, 
      Gifu, Japan.
FAU - Kuwata, Hirotaka
AU  - Kuwata H
AD  - Department of Oral Microbiology and Immunology, School of Dentistry, Showa 
      University, Shinagawa, Tokyo, Japan.
FAU - Kawabata, Shigetada
AU  - Kawabata S
AD  - Department of Oral and Molecular Microbiology, Osaka University Graduate School 
      of Dentistry, Suita, Osaka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200417
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Allergens)
RN  - 0 (Dinitrophenols)
RN  - 0 (IL4 protein, human)
RN  - 0 (Plant Extracts)
RN  - 0 (Sugars)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Allergens/immunology/*metabolism
MH  - Animals
MH  - Basophils/immunology/microbiology/pathology
MH  - Cell Degranulation/immunology
MH  - Cell Survival/immunology
MH  - Dinitrophenols/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Hypersensitivity/drug therapy/*immunology/pathology
MH  - Immunoglobulin E/*immunology/metabolism
MH  - Interleukin-4/genetics/metabolism
MH  - Mast Cells/immunology/microbiology/pathology
MH  - Mice
MH  - Plant Extracts/chemistry/pharmacology
MH  - Serum Albumin, Human/immunology/metabolism
MH  - Streptococcal Infections/*drug therapy/immunology
MH  - Streptococcus oralis/immunology/pathogenicity
MH  - Sugars/metabolism
PMC - PMC7164662
COIS- The authors have declared that no competing interests exist.
EDAT- 2020/04/18 06:00
MHDA- 2020/07/09 06:00
PMCR- 2020/04/17
CRDT- 2020/04/18 06:00
PHST- 2020/01/10 00:00 [received]
PHST- 2020/03/16 00:00 [accepted]
PHST- 2020/04/18 06:00 [entrez]
PHST- 2020/04/18 06:00 [pubmed]
PHST- 2020/07/09 06:00 [medline]
PHST- 2020/04/17 00:00 [pmc-release]
AID - PONE-D-20-00823 [pii]
AID - 10.1371/journal.pone.0231101 [doi]
PST - epublish
SO  - PLoS One. 2020 Apr 17;15(4):e0231101. doi: 10.1371/journal.pone.0231101. 
      eCollection 2020.