PMID- 32302971
OWN - NLM
STAT- MEDLINE
DCOM- 20210125
LR  - 20210125
IS  - 1479-6805 (Electronic)
IS  - 0022-0795 (Linking)
VI  - 246
IP  - 1
DP  - 2020 Jul
TI  - TGFbeta1 regulates prolactin secretion during postnatal development: gender 
      differences.
PG  - 29-39
LID - 10.1530/JOE-20-0041 [doi]
AB  - Serum prolactin levels gradually increase from birth to puberty in both male and 
      female rats, with higher levels observed in female since the first days of life. 
      The increase in lactotroph secretion was attributed to the maturation of 
      prolactin-inhibiting and prolactin-releasing factors; however, those mechanisms 
      could not fully explain the gender differences observed. Prolactin secretion from 
      isolated lactotrophs, in the absence of hypothalamic control, also increases 
      during the first weeks of life, suggesting the involvement of intra-pituitary 
      factors. We postulate that pituitary transforming growth factor beta 1 (TGFbeta1) is 
      involved in the regulation of prolactin secretion as well as in the gender 
      differences observed at early postnatal age. Several components of the local 
      TGFbeta1 system were evaluated during postnatal development (11, 23, and 45 days) in 
      female and male Sprague-Dawley rats. In vivo assays were performed to study local 
      TGFbeta1 activation and its impact on prolactin secretion. At day 11, female 
      pituitaries present high levels of active TGFbeta1, concomitant with the highest 
      expression of TGFbeta1 target genes and the phospho-Smad3 immunostaining in 
      lactotrophs. The steady increase in prolactin secretion inversely correlates with 
      active TGFbeta1 levels only in females. Dopamine and estradiol induce TGFbeta1 
      activation at day 11, in both genders, but its activation induces the inhibition 
      of prolactin secretion only in females. Our findings demonstrate that: (1) TGFbeta1 
      activation is regulated by dopamine and estradiol; (2) the inhibitory regulation 
      of local TGFbeta1 on prolactin secretion is gender specific; and (3) this mechanism 
      is responsible, at least partially, for the gender differences observed being 
      relevant during postnatal development.
FAU - Abeledo-Machado, Alejandra
AU  - Abeledo-Machado A
AD  - Instituto de Biologia y Medicina Experimental (IBYME), CONICET, Buenos Aires, 
      Argentina.
FAU - Perez, Pablo Anibal
AU  - Perez PA
AD  - Centro de Microscopia Electronica, Instituto de Investigaciones en Ciencias de la 
      Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de 
      Cordoba, Cordoba, Argentina.
FAU - Camilletti, Maria Andrea
AU  - Camilletti MA
AD  - Instituto de Biologia y Medicina Experimental (IBYME), CONICET, Buenos Aires, 
      Argentina.
FAU - Faraoni, Erika Yanil
AU  - Faraoni EY
AD  - Instituto de Biologia y Medicina Experimental (IBYME), CONICET, Buenos Aires, 
      Argentina.
FAU - Picech, Florencia
AU  - Picech F
AD  - Centro de Microscopia Electronica, Instituto de Investigaciones en Ciencias de la 
      Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de 
      Cordoba, Cordoba, Argentina.
FAU - Petiti, Juan Pablo
AU  - Petiti JP
AD  - Centro de Microscopia Electronica, Instituto de Investigaciones en Ciencias de la 
      Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de 
      Cordoba, Cordoba, Argentina.
FAU - Gutierrez, Silvina
AU  - Gutierrez S
AD  - Centro de Microscopia Electronica, Instituto de Investigaciones en Ciencias de la 
      Salud (INICSA-CONICET), Facultad de Ciencias Medicas, Universidad Nacional de 
      Cordoba, Cordoba, Argentina.
FAU - Diaz-Torga, Graciela
AU  - Diaz-Torga G
AD  - Instituto de Biologia y Medicina Experimental (IBYME), CONICET, Buenos Aires, 
      Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Smad3 Protein)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-62-4 (Prolactin)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Dopamine/pharmacology
MH  - Estradiol/pharmacology
MH  - Female
MH  - Lactotrophs/drug effects/metabolism
MH  - Male
MH  - Prolactin/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sex Characteristics
MH  - Smad3 Protein/metabolism
MH  - Transforming Growth Factor beta1/*metabolism
OTO - NOTNLM
OT  - TGFbeta1
OT  - gender differences
OT  - lactotrophs
OT  - postnatal development
OT  - prolactin
EDAT- 2020/04/18 06:00
MHDA- 2021/01/26 06:00
CRDT- 2020/04/18 06:00
PHST- 2020/03/06 00:00 [received]
PHST- 2020/04/09 00:00 [accepted]
PHST- 2020/04/18 06:00 [pubmed]
PHST- 2021/01/26 06:00 [medline]
PHST- 2020/04/18 06:00 [entrez]
AID - JOE-20-0041.R1 [pii]
AID - 10.1530/JOE-20-0041 [doi]
PST - ppublish
SO  - J Endocrinol. 2020 Jul;246(1):29-39. doi: 10.1530/JOE-20-0041.