PMID- 32304687
OWN - NLM
STAT- MEDLINE
DCOM- 20210901
LR  - 20210901
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1740
DP  - 2020 Aug 1
TI  - Electroacupuncture improves cognitive deficits and insulin resistance in an OLETF 
      rat model of Al/D-gal induced aging model via the PI3K/Akt signaling pathway.
PG  - 146834
LID - S0006-8993(20)30190-6 [pii]
LID - 10.1016/j.brainres.2020.146834 [doi]
AB  - To investigate the effect of electroacupuncture (EA) on cognitive function and 
      insulin resistance (IR) in an Al/D-gal-induced aging model for Alzheimer's 
      disease (AD) using Ostuka Long-Evans Tokushima Fatty (OLETF) rats. The 
      Al/D-gal-OLETF rats for AD were randomly divided into the EA and non-EA groups. 
      Cognitive function was assessed using the Morris water maze (MWM). The morphology 
      of the hippocampal neurons was observed using hematoxylin & eosin (H&E) staining. 
      Abeta and total Tau in the hippocampus and cerebrospinal fluid (CSF) were detected 
      using western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). 
      Fasting blood glucose (FPG) was determined using the glucose oxidase method. 
      Plasma fasting insulin (FINS), serum C-peptide (C-P), and CSF insulin were 
      detected using ELISA. The expression of the genes and proteins in the PI3K 
      signaling pathway was detected using quantitative real-time PCR and WB. After EA 
      intervention, the hippocampal Abeta and total Tau protein levels, body weight, FPG, 
      FINS, and C-P were significantly decreased. The MWM showed that the percentage of 
      time in the target quadrant of the EA group was elevated in the probe test. The 
      protein levels of p-IRS1, p-IRS2, IDE, and p-GSK3beta were significantly increased, 
      while p-PI3K-p85alpha and p-Akt were decreased. In conclusion, EA improves cognitive 
      function and insulin resistance in rat models of AD. The PI3K/Akt signaling 
      pathway is involved in those changes.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Huang, Xinyu
AU  - Huang X
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Huang, Kangbai
AU  - Huang K
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Li, Zhaowei
AU  - Li Z
AD  - College of Sport and Healthy, Guangzhou University of Chinese Medicine, 
      Guangzhou, Guangdong 510006, China.
FAU - Bai, Dongyan
AU  - Bai D
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Hao, Yan
AU  - Hao Y
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Wu, Qinglong
AU  - Wu Q
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Yi, Wei
AU  - Yi W
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Xu, Nenggui
AU  - Xu N
AD  - Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Pan, Youcan
AU  - Pan Y
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
FAU - Zhang, Liang
AU  - Zhang L
AD  - Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, 
      Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China. 
      Electronic address: zhangliang307@gzucm.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200415
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - X2RN3Q8DNE (Galactose)
SB  - IM
MH  - Aging/genetics/*metabolism
MH  - Alzheimer Disease/genetics/metabolism/therapy
MH  - Animals
MH  - Cognitive Dysfunction/genetics/*metabolism/therapy
MH  - Electroacupuncture/*methods
MH  - Galactose/genetics/metabolism
MH  - Insulin Resistance/*physiology
MH  - Male
MH  - Maze Learning/physiology
MH  - Phosphatidylinositol 3-Kinases/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-akt/genetics/*metabolism
MH  - Rats
MH  - Rats, Inbred OLETF
MH  - Rats, Long-Evans
MH  - Signal Transduction/physiology
OTO - NOTNLM
OT  - Acupuncture
OT  - Alzheimer's disease
OT  - Insulin resistance
OT  - Ostuka Long-Evans Tokushima Fatty rats
OT  - beta-Amyloid
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/04/19 06:00
MHDA- 2021/09/02 06:00
CRDT- 2020/04/19 06:00
PHST- 2020/01/14 00:00 [received]
PHST- 2020/03/25 00:00 [revised]
PHST- 2020/04/13 00:00 [accepted]
PHST- 2020/04/19 06:00 [pubmed]
PHST- 2021/09/02 06:00 [medline]
PHST- 2020/04/19 06:00 [entrez]
AID - S0006-8993(20)30190-6 [pii]
AID - 10.1016/j.brainres.2020.146834 [doi]
PST - ppublish
SO  - Brain Res. 2020 Aug 1;1740:146834. doi: 10.1016/j.brainres.2020.146834. Epub 2020 
      Apr 15.