PMID- 32309446
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20220414
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2020
DP  - 2020
TI  - Roux-en-Y Gastric Bypass in Obese Diabetic Rats Promotes Autophagy to Improve 
      Lipid Metabolism through mTOR/p70S6K Signaling Pathway.
PG  - 4326549
LID - 10.1155/2020/4326549 [doi]
LID - 4326549
AB  - PURPOSE: To investigate the effects of Roux-en-Y gastric bypass (RYGB) surgery on 
      markers of liver mitochondrial dynamics and find new therapeutic basis on obese 
      type 2 diabetes mellitus (T2DM) patients. Materials and Methods. Thirty-two rats 
      were divided into nondiabetic group, diabetic group, sham group, and RYGB group. 
      The Dual-energy X-ray absorptiometry (DEXA) was used to detect short-term 
      curriculum vitae for rat body component and fat and lean mass. Hepatic lipid 
      content and triglyceride levels were detected by Oil Red O staining. Western 
      blotting was used to examine autophagy and mammalian target of rapamycin/P70S6 
      kinase (mTOR/p70S6K) pathway-related proteins. The carbon dioxide production from 
      the oxidation of [(14)C] oleate was measured. Plasma glucose was measured by 
      glucose oxidase assay. The insulin and C-peptide were detected. Triacylglyceride 
      (TG) and free fat acid (FFA) in plasma were determined by enzymatic colorimetric 
      assays. RESULTS: RYGB improved metabolic parameters and enhanced plasma GLP-1 
      level, ameliorated the lipopexia, and increased insulin sensitivity in the liver; 
      RYGB promoted the hepatic autophagy and inhibited the mTOR/p70S6K signaling 
      pathway. GLP-1 reduced fat load and increased fatty acid beta-oxidation by activated 
      autophagy to regulate the hepatic lipid pathway through mTOR/p70S6K signaling 
      pathway. CONCLUSIONS: RYGB may reduce liver lipid toxicity and improve insulin 
      sensitivity through activating the hepatic fat hydrolysis pathway and inhibiting 
      the liver fat synthesis pathway. However, the transport pathway of liver fat does 
      not play a key role.
CI  - Copyright (c) 2020 Nanxi Ma et al.
FAU - Ma, Nanxi
AU  - Ma N
AD  - Department of Endocrinology, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, China.
FAU - Ma, Rui
AU  - Ma R
AD  - Department of Endocrinology, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, China.
FAU - Tang, Kaixin
AU  - Tang K
AD  - Department of Endocrinology, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, China.
FAU - Li, Xuesong
AU  - Li X
AD  - Department of Endocrinology, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, China.
FAU - He, Bing
AU  - He B
AUID- ORCID: 0000-0002-9694-6591
AD  - Department of Endocrinology, Shengjing Hospital of China Medical University, 
      Shenyang, Liaoning 110004, China.
LA  - eng
PT  - Journal Article
DEP - 20200326
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (Blood Glucose)
RN  - 89750-14-1 (Glucagon-Like Peptide 1)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Autophagy/*physiology
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Experimental/metabolism/*surgery
MH  - *Gastric Bypass
MH  - Glucagon-Like Peptide 1/blood
MH  - Insulin Resistance/physiology
MH  - Lipid Metabolism/*physiology
MH  - Male
MH  - Obesity/metabolism/*surgery
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - Signal Transduction/*physiology
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC7136782
COIS- The authors declare that they have no competing interests.
EDAT- 2020/04/21 06:00
MHDA- 2021/02/17 06:00
PMCR- 2020/03/26
CRDT- 2020/04/21 06:00
PHST- 2019/10/10 00:00 [received]
PHST- 2020/01/28 00:00 [revised]
PHST- 2020/02/12 00:00 [accepted]
PHST- 2020/04/21 06:00 [entrez]
PHST- 2020/04/21 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/03/26 00:00 [pmc-release]
AID - 10.1155/2020/4326549 [doi]
PST - epublish
SO  - J Diabetes Res. 2020 Mar 26;2020:4326549. doi: 10.1155/2020/4326549. eCollection 
      2020.