PMID- 32311846
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20201130
IS  - 1099-0496 (Electronic)
IS  - 1099-0496 (Linking)
VI  - 55
IP  - 6
DP  - 2020 Jun
TI  - Analysis of 25-hydroxy cholecalciferol, immunoglobulin E, and vitamin D receptor 
      single nucleotide polymorphisms (Apa1, Taq1, and Bsm1), among sample of Egyptian 
      children with bronchial asthma: A case-control study.
PG  - 1349-1358
LID - 10.1002/ppul.24785 [doi]
AB  - PURPOSE: The current study aimed to assess the serum levels of vitamin D and 
      immunoglobulin E (IgE) among asthmatic Egyptian children and to find out the 
      possible associations of vitamin D receptor (VDR) polymorphisms with bronchial 
      asthma development. METHODS: The study included 100 Egyptian children, 50 
      asthmatic children who were comparable with 50 age, sex, and body mass 
      index-matched, unrelated healthy controls (HCs) clinical assessments of asthmatic 
      children were done using global initiative of asthma. Pulmonary function tests 
      (forced expiratory volume in 1 second [FEV1], forced vital capacity [FVC], 
      FEV1/FVC ratio) were performed. Enzyme-linked immunosorbent assays of serum 
      vitamin D3 and total IgE were done. VDR-single nucleotide polymorphisms (SNPs) 
      (ApaI, TaqI, and BsmI) detection has performed using polymerase chain reaction 
      through restriction fragment length polymorphism technique. Data analysis was 
      performed using SPSS version 20.0. The studied SNPs were followed the Hardy 
      Weinberg equation. RESULTS: The mean serum level of 25(OH) D3 was significantly 
      lower among asthmatic children (13.46 ng/mL +/- 10.50 SD) in comparison to HCs 
      (37.53 ng/mL +/- 13. 0.40 SD), P < .05. Vitamin D deficiency was detected in 72% of 
      cases with no significant difference in its level regarding asthma control. There 
      was significantly higher IgE level among asthmatic children 
      (99.83 ku/L +/- 233.81 SD) versus HCs (7.52 ku/L +/- 3.32 SD), P < .05. Asthmatic 
      children were presented more commonly with TaqI t allele (odds ratio [OR], 2.25; 
      95% confidence interval [CI], 1.28-3.96; P < .05) and BsmI b allele (OR, 1.83; 
      95% CI, 1.05-3.21; P < .05). ApaI a allele was not significantly different among 
      patients versus controls (P > .05). TT + Tt and Bb + bb genotypes were 
      significantly higher among cases versus the controls, P < .05 for all. 
      CONCLUSIONS: TaqI and BsmI were associated with risk of bronchial asthma 
      development among Egyptian children. High IgE and Low vitamin D status were 
      frequently occurring among asthmatic children.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Ahmed, Ahmed El-Abd
AU  - Ahmed AE
AUID- ORCID: 0000-0002-1843-5411
AD  - Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, 
      Egypt.
FAU - Hassan, Mohammed H
AU  - Hassan MH
AUID- ORCID: 0000-0003-2698-9438
AD  - Department of Medical Biochemistry, Faculty of Medicine, South Valley University, 
      Qena, Egypt.
FAU - Toghan, Rana
AU  - Toghan R
AUID- ORCID: 0000-0002-7254-6104
AD  - Department of Medical Physiology, Faculty of Medicine, South Valley University, 
      Qena, Egypt.
FAU - Rashwan, Nagwan I
AU  - Rashwan NI
AUID- ORCID: 0000-0002-7497-0398
AD  - Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, 
      Egypt.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200420
PL  - United States
TA  - Pediatr Pulmonol
JT  - Pediatric pulmonology
JID - 8510590
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (VDR protein, human)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - P6YZ13C99Q (Calcifediol)
SB  - IM
MH  - Adolescent
MH  - Alleles
MH  - Asthma/*blood/*genetics
MH  - Calcifediol/*blood
MH  - Case-Control Studies
MH  - Child
MH  - Egypt
MH  - Female
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Odds Ratio
MH  - Polymorphism, Single Nucleotide
MH  - Receptors, Calcitriol/*genetics
MH  - Vitamin D Deficiency/blood/genetics
OTO - NOTNLM
OT  - IgE
OT  - bronchial asthma
OT  - egyptian children
OT  - genomic DNA
OT  - vitamin D receptor single nucleotide polymorphisms
OT  - vitamin D3
EDAT- 2020/04/21 06:00
MHDA- 2020/12/01 06:00
CRDT- 2020/04/21 06:00
PHST- 2019/12/30 00:00 [received]
PHST- 2020/04/09 00:00 [revised]
PHST- 2020/04/10 00:00 [accepted]
PHST- 2020/04/21 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/04/21 06:00 [entrez]
AID - 10.1002/ppul.24785 [doi]
PST - ppublish
SO  - Pediatr Pulmonol. 2020 Jun;55(6):1349-1358. doi: 10.1002/ppul.24785. Epub 2020 
      Apr 20.