PMID- 32314600
OWN - NLM
STAT- MEDLINE
DCOM- 20210316
LR  - 20210316
IS  - 1477-0903 (Electronic)
IS  - 0960-3271 (Linking)
VI  - 39
IP  - 9
DP  - 2020 Sep
TI  - Action of the insecticide cyfluthrin on Ca(2+) signal transduction and 
      cytotoxicity in human osteosarcoma cells.
PG  - 1268-1276
LID - 10.1177/0960327120918298 [doi]
AB  - Cyfluthrin is a pyrethroid insecticide and common household pesticide. The effect 
      of cyfluthrin on Ca(2+)-related physiology in human osteosarcoma is unclear. This 
      study investigated the effect of cyfluthrin on cytosolic-free Ca(2+) 
      concentrations ([Ca(2+)](i)) and viability in MG63 human osteosarcoma cells. 
      Cyfluthrin concentration-dependently induced [Ca(2+)](i) rises. 
      Cyfluthrin-induced Ca(2+) entry was confirmed by the Mn(2+)-induced quench of 
      fura-2 fluorescence. Cyfluthrin at concentrations of 10-100 muM induced 
      [Ca(2+)](i) rises. Ca(2+) removal reduced the signal by approximately 50%. 
      Cyfluthrin (100 muM) induced Mn(2+) influx suggesting Ca(2+) entry. 
      Cyfluthrin-induced Ca(2+) entry was inhibited 50% by protein kinase C (PKC) 
      activator (phorbol 12-myristate 13-acetate) and inhibitor (GF109203X) and also by 
      three inhibitors of store-operated Ca(2+) channels: nifedipine, econazole, and 
      SKF96365. In Ca(2+)-free medium, treatment with the endoplasmic reticulum Ca(2+) 
      pump inhibitor thapsigargin (TG) completely inhibited cyfluthrin-evoked 
      [Ca(2+)](i) rises. Conversely, treatment with cyfluthrin abolished TG-evoked 
      [Ca(2+)](i) rises. Inhibition of phospholipase C (PLC) with 
      1-[6-[((17beta)-3-methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dion 
      abolished cyfluthrin-induced [Ca(2+)](i) rises. Cyfluthrin at 25-65 muM decreased 
      cell viability, which was not reversed by pretreatment with the Ca(2+) chelator 
      1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester. 
      Together, in MG63 cells, cyfluthrin induced [Ca(2+)](i) rises by evoking 
      PLC-dependent Ca(2+) release from the endoplasmic reticulum and Ca(2+) entry via 
      PKC-sensitive store-operated Ca(2+) entry. Cyfluthrin also caused 
      Ca(2+)-independent cell death.
FAU - Lu, Y-C
AU  - Lu YC
AD  - Department of Orthopedics, Kaohsiung Veterans General Hospital, Kaohsiung.
FAU - Liang, W-Z
AU  - Liang WZ
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung.
AD  - Department of Pharmacy, Tajen University, Pingtung.
FAU - Kuo, C-C
AU  - Kuo CC
AD  - Department of Nursing, Tzu Hui Institute of Technology, Pingtung.
FAU - Hao, L-J
AU  - Hao LJ
AD  - Department of Endocrinology and Metabolism, Kaohsiung Veterans General Hospital 
      Tainan Branch, Tainan.
AD  - Chung Hwa University of Medical and Technology, Tainan.
FAU - Chou, C-T
AU  - Chou CT
AD  - Department of Nursing, Division of Basic Medical Sciences, Chang Gung University 
      of Science and Technology, Chiayi Campus, Puzi City, Chiayi County.
AD  - Division of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital 
      Chiayi Branch, Puzi City, Chiayi County.
FAU - Jan, C-R
AU  - Jan CR
AUID- ORCID: 0000-0003-4570-4709
AD  - Department of Medical Education and Research, Kaohsiung Veterans General 
      Hospital, Kaohsiung.
LA  - eng
PT  - Journal Article
DEP - 20200421
PL  - England
TA  - Hum Exp Toxicol
JT  - Human & experimental toxicology
JID - 9004560
RN  - 0 (Insecticides)
RN  - 0 (Nitriles)
RN  - 0 (Pyrethrins)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SCM2QLZ6S0 (cyfluthrin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/analysis
MH  - Calcium Signaling/*drug effects
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Humans
MH  - Insecticides/*toxicity
MH  - Nitriles/*toxicity
MH  - Osteosarcoma
MH  - Pyrethrins/*toxicity
MH  - Type C Phospholipases/metabolism
OTO - NOTNLM
OT  - Ca2+
OT  - cyfluthrin
OT  - endoplasmic reticulum
OT  - human osteosarcoma cells
OT  - phospholipase C
EDAT- 2020/04/22 06:00
MHDA- 2021/03/17 06:00
CRDT- 2020/04/22 06:00
PHST- 2020/04/22 06:00 [pubmed]
PHST- 2021/03/17 06:00 [medline]
PHST- 2020/04/22 06:00 [entrez]
AID - 10.1177/0960327120918298 [doi]
PST - ppublish
SO  - Hum Exp Toxicol. 2020 Sep;39(9):1268-1276. doi: 10.1177/0960327120918298. Epub 
      2020 Apr 21.